Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil

PURPOSE: The aim of the present study was to detect and genotype T. gondii in free-range chickens destined to human consumption in Alagoas state, Brazil. METHODS: Two hundred blood samples were collected from free-range chickens and submitted to indirect immunofluorescence antibody test (IFAT). Brai...

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Autores principales: dos Santos Silva, Ana Clécia, de Barros, Luiz Daniel, Barros, Viviane Melo Coelho, de Alcântara, Adrianne Mota, Andrade, Müller Ribeiro, Garcia, João Luis, Mota, Rinaldo Aparecido, Porto, Wagnner José Nascimento
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2020
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Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223798/
https://www.ncbi.nlm.nih.gov/pubmed/32141021
http://dx.doi.org/10.2478/s11686-020-00194-2
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author dos Santos Silva, Ana Clécia
de Barros, Luiz Daniel
Barros, Viviane Melo Coelho
de Alcântara, Adrianne Mota
Andrade, Müller Ribeiro
Garcia, João Luis
Mota, Rinaldo Aparecido
Porto, Wagnner José Nascimento
author_facet dos Santos Silva, Ana Clécia
de Barros, Luiz Daniel
Barros, Viviane Melo Coelho
de Alcântara, Adrianne Mota
Andrade, Müller Ribeiro
Garcia, João Luis
Mota, Rinaldo Aparecido
Porto, Wagnner José Nascimento
author_sort dos Santos Silva, Ana Clécia
collection PubMed
description PURPOSE: The aim of the present study was to detect and genotype T. gondii in free-range chickens destined to human consumption in Alagoas state, Brazil. METHODS: Two hundred blood samples were collected from free-range chickens and submitted to indirect immunofluorescence antibody test (IFAT). Brain tissue from 14 animals randomly selected were subjected to mouse bioassay. Positive samples in mouse bioassay were submitted to PCR and genotyped by PCR–RFLP. RESULTS: Out of two hundred blood samples from chickens, 72 (36%) samples were considered positive by IFAT. Two T. gondii strains were isolated, both being characterized as atypical and classified as #146 and a new genotype, named #279 in ToxoDB database. CONCLUSIONS: Our results showed a sero-occurrence of T. gondii in free-range chickens intended for humans, and the genetic diversity of the parasite in Brazil, with a new genotype described.
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spelling pubmed-72237982020-05-15 Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil dos Santos Silva, Ana Clécia de Barros, Luiz Daniel Barros, Viviane Melo Coelho de Alcântara, Adrianne Mota Andrade, Müller Ribeiro Garcia, João Luis Mota, Rinaldo Aparecido Porto, Wagnner José Nascimento Acta Parasitol Short Communication PURPOSE: The aim of the present study was to detect and genotype T. gondii in free-range chickens destined to human consumption in Alagoas state, Brazil. METHODS: Two hundred blood samples were collected from free-range chickens and submitted to indirect immunofluorescence antibody test (IFAT). Brain tissue from 14 animals randomly selected were subjected to mouse bioassay. Positive samples in mouse bioassay were submitted to PCR and genotyped by PCR–RFLP. RESULTS: Out of two hundred blood samples from chickens, 72 (36%) samples were considered positive by IFAT. Two T. gondii strains were isolated, both being characterized as atypical and classified as #146 and a new genotype, named #279 in ToxoDB database. CONCLUSIONS: Our results showed a sero-occurrence of T. gondii in free-range chickens intended for humans, and the genetic diversity of the parasite in Brazil, with a new genotype described. Springer International Publishing 2020-03-05 2020 /pmc/articles/PMC7223798/ /pubmed/32141021 http://dx.doi.org/10.2478/s11686-020-00194-2 Text en © Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Short Communication
dos Santos Silva, Ana Clécia
de Barros, Luiz Daniel
Barros, Viviane Melo Coelho
de Alcântara, Adrianne Mota
Andrade, Müller Ribeiro
Garcia, João Luis
Mota, Rinaldo Aparecido
Porto, Wagnner José Nascimento
Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil
title Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil
title_full Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil
title_fullStr Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil
title_full_unstemmed Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil
title_short Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil
title_sort occurrence of atypical and new genotypes of toxoplasma gondii in free-range chickens intended for human consumption in brazil
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223798/
https://www.ncbi.nlm.nih.gov/pubmed/32141021
http://dx.doi.org/10.2478/s11686-020-00194-2
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