rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts

Toxoplasma gondii oocyst wall protein 1 (TgOWP1) integrates a family of seven proteins, consensually assumed as specific antigens of Toxoplasma gondii oocyst stage, located in the outer layer of the oocyst wall. Herein, we notice the expression of a recombinant antigen, rTgOWP1-f, derived from a fra...

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Autores principales: Sousa, Susana, Almeida, André, Delgado, Lurdes, Conceição, Antónia, Marques, Cláudia, da Costa, José Manuel Correia, Castro, António
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7224171/
https://www.ncbi.nlm.nih.gov/pubmed/32409659
http://dx.doi.org/10.1038/s41598-020-64590-4
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author Sousa, Susana
Almeida, André
Delgado, Lurdes
Conceição, Antónia
Marques, Cláudia
da Costa, José Manuel Correia
Castro, António
author_facet Sousa, Susana
Almeida, André
Delgado, Lurdes
Conceição, Antónia
Marques, Cláudia
da Costa, José Manuel Correia
Castro, António
author_sort Sousa, Susana
collection PubMed
description Toxoplasma gondii oocyst wall protein 1 (TgOWP1) integrates a family of seven proteins, consensually assumed as specific antigens of Toxoplasma gondii oocyst stage, located in the outer layer of the oocyst wall. Herein, we notice the expression of a recombinant antigen, rTgOWP1-f, derived from a fragment selected on basis of its structural homology with Plasmodium MSP1–19. Rabbit polyclonal antibodies anti-rTgOWP1-f evidence ability for specific identification of environmental T. gondii oocysts. We assume, rTgOWP1-f, as a possible biomarker of oocysts. In addition, we present findings supporting this vision, including the development of an immunodetection method for T. gondii oocysts identification.
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spelling pubmed-72241712020-05-20 rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts Sousa, Susana Almeida, André Delgado, Lurdes Conceição, Antónia Marques, Cláudia da Costa, José Manuel Correia Castro, António Sci Rep Article Toxoplasma gondii oocyst wall protein 1 (TgOWP1) integrates a family of seven proteins, consensually assumed as specific antigens of Toxoplasma gondii oocyst stage, located in the outer layer of the oocyst wall. Herein, we notice the expression of a recombinant antigen, rTgOWP1-f, derived from a fragment selected on basis of its structural homology with Plasmodium MSP1–19. Rabbit polyclonal antibodies anti-rTgOWP1-f evidence ability for specific identification of environmental T. gondii oocysts. We assume, rTgOWP1-f, as a possible biomarker of oocysts. In addition, we present findings supporting this vision, including the development of an immunodetection method for T. gondii oocysts identification. Nature Publishing Group UK 2020-05-14 /pmc/articles/PMC7224171/ /pubmed/32409659 http://dx.doi.org/10.1038/s41598-020-64590-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sousa, Susana
Almeida, André
Delgado, Lurdes
Conceição, Antónia
Marques, Cláudia
da Costa, José Manuel Correia
Castro, António
rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
title rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
title_full rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
title_fullStr rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
title_full_unstemmed rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
title_short rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
title_sort rtgowp1-f, a specific biomarker for toxoplasma gondii oocysts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7224171/
https://www.ncbi.nlm.nih.gov/pubmed/32409659
http://dx.doi.org/10.1038/s41598-020-64590-4
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