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Serological evidence for human exposure to Bacillus cereus biovar anthracis in the villages around Taï National Park, Côte d’Ivoire
Bacillus cereus biovar anthracis (Bcbva) is an untypical anthrax-causing pathogen responsible for high wildlife mortality in Taï National Park (TNP), Côte d’Ivoire. However, nothing is known about its effect on the rural population living in the region bordering TNP. Contact to bushmeat is a known r...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7224451/ https://www.ncbi.nlm.nih.gov/pubmed/32407387 http://dx.doi.org/10.1371/journal.pntd.0008292 |
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author | Dupke, Susann Schubert, Grit Beudjé, Félicité Barduhn, Anne Pauly, Maude Couacy-Hymann, Emmanuel Grunow, Roland Akoua-Koffi, Chantal Leendertz, Fabian H. Klee, Silke R. |
author_facet | Dupke, Susann Schubert, Grit Beudjé, Félicité Barduhn, Anne Pauly, Maude Couacy-Hymann, Emmanuel Grunow, Roland Akoua-Koffi, Chantal Leendertz, Fabian H. Klee, Silke R. |
author_sort | Dupke, Susann |
collection | PubMed |
description | Bacillus cereus biovar anthracis (Bcbva) is an untypical anthrax-causing pathogen responsible for high wildlife mortality in Taï National Park (TNP), Côte d’Ivoire. However, nothing is known about its effect on the rural population living in the region bordering TNP. Contact to bushmeat is a known risk factor for exposure to a variety of zoonotic pathogens, but no human infections with Bcbva were noted so far. Therefore, we performed a retrospective seroprevalence analysis with sera from 1,386 study volunteers. We used assays which detect antibodies against the protective antigen PA, which is synthesized by both Bcbva and classic B. anthracis, and against the recently described antigen pXO2-60, a 35-kDa protein only produced by Bcbva. We found a high seroprevalence (22.37%) of antibodies against PA, and approximately half of those sera (10.46%) were also positive for the Bcbva-specific antigen pXO2-60. All sera negative for PA were also negative for antibodies against pXO2-60, confirming specificity and suitability of the PA/pXO2-60 combined serological assay. The fact that a large fraction of sera was positive for PA but negative for pXO2-60 can most likely be explained by lower immunogenicity of pXO2-60, but exposure to classic B. anthracis cannot be excluded. As only Bcbva has been detected in the TNP area so far, exposure to Bcbva can be suspected from the presence of antibodies against PA alone. In a questionnaire, most study participants reported contact to bushmeat and livestock carcasses. Unfortunately, risk factor analysis indicated that neither animal contacts, sex, age, nor country of origin were significant predictors of Bcbva seroprevalence. Nevertheless, our study added to an assessment of the distribution of Bcbva and its impact on the human population, and our data can serve to raise awareness of anthrax in the affected regions. |
format | Online Article Text |
id | pubmed-7224451 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-72244512020-06-01 Serological evidence for human exposure to Bacillus cereus biovar anthracis in the villages around Taï National Park, Côte d’Ivoire Dupke, Susann Schubert, Grit Beudjé, Félicité Barduhn, Anne Pauly, Maude Couacy-Hymann, Emmanuel Grunow, Roland Akoua-Koffi, Chantal Leendertz, Fabian H. Klee, Silke R. PLoS Negl Trop Dis Research Article Bacillus cereus biovar anthracis (Bcbva) is an untypical anthrax-causing pathogen responsible for high wildlife mortality in Taï National Park (TNP), Côte d’Ivoire. However, nothing is known about its effect on the rural population living in the region bordering TNP. Contact to bushmeat is a known risk factor for exposure to a variety of zoonotic pathogens, but no human infections with Bcbva were noted so far. Therefore, we performed a retrospective seroprevalence analysis with sera from 1,386 study volunteers. We used assays which detect antibodies against the protective antigen PA, which is synthesized by both Bcbva and classic B. anthracis, and against the recently described antigen pXO2-60, a 35-kDa protein only produced by Bcbva. We found a high seroprevalence (22.37%) of antibodies against PA, and approximately half of those sera (10.46%) were also positive for the Bcbva-specific antigen pXO2-60. All sera negative for PA were also negative for antibodies against pXO2-60, confirming specificity and suitability of the PA/pXO2-60 combined serological assay. The fact that a large fraction of sera was positive for PA but negative for pXO2-60 can most likely be explained by lower immunogenicity of pXO2-60, but exposure to classic B. anthracis cannot be excluded. As only Bcbva has been detected in the TNP area so far, exposure to Bcbva can be suspected from the presence of antibodies against PA alone. In a questionnaire, most study participants reported contact to bushmeat and livestock carcasses. Unfortunately, risk factor analysis indicated that neither animal contacts, sex, age, nor country of origin were significant predictors of Bcbva seroprevalence. Nevertheless, our study added to an assessment of the distribution of Bcbva and its impact on the human population, and our data can serve to raise awareness of anthrax in the affected regions. Public Library of Science 2020-05-14 /pmc/articles/PMC7224451/ /pubmed/32407387 http://dx.doi.org/10.1371/journal.pntd.0008292 Text en © 2020 Dupke et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Dupke, Susann Schubert, Grit Beudjé, Félicité Barduhn, Anne Pauly, Maude Couacy-Hymann, Emmanuel Grunow, Roland Akoua-Koffi, Chantal Leendertz, Fabian H. Klee, Silke R. Serological evidence for human exposure to Bacillus cereus biovar anthracis in the villages around Taï National Park, Côte d’Ivoire |
title | Serological evidence for human exposure to Bacillus cereus biovar anthracis in the villages around Taï National Park, Côte d’Ivoire |
title_full | Serological evidence for human exposure to Bacillus cereus biovar anthracis in the villages around Taï National Park, Côte d’Ivoire |
title_fullStr | Serological evidence for human exposure to Bacillus cereus biovar anthracis in the villages around Taï National Park, Côte d’Ivoire |
title_full_unstemmed | Serological evidence for human exposure to Bacillus cereus biovar anthracis in the villages around Taï National Park, Côte d’Ivoire |
title_short | Serological evidence for human exposure to Bacillus cereus biovar anthracis in the villages around Taï National Park, Côte d’Ivoire |
title_sort | serological evidence for human exposure to bacillus cereus biovar anthracis in the villages around taï national park, côte d’ivoire |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7224451/ https://www.ncbi.nlm.nih.gov/pubmed/32407387 http://dx.doi.org/10.1371/journal.pntd.0008292 |
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