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Cuminaldehyde potentiates the antimicrobial actions of ciprofloxacin against Staphylococcus aureus and Escherichia coli
Escherichia coli and Staphylococcus aureus are important agents of urinary tract infections that can often evolve to severe infections. The rise of antibiotic-resistant strains has driven the search for novel therapies to replace the use or act as adjuvants of antibiotics. In this context, plant-der...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7224478/ https://www.ncbi.nlm.nih.gov/pubmed/32407399 http://dx.doi.org/10.1371/journal.pone.0232987 |
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author | Monteiro-Neto, Valério de Souza, Cláudio D. Gonzaga, Laoane F. da Silveira, Bruna C. Sousa, Nágila C. F. Pontes, Jaqueline P. Santos, Deivid M. Martins, Wanessa C. Pessoa, Jorge F. V. Carvalho Júnior, Alexsander R. Almeida, Viviane S. S. de Oliveira, Natália M. T. de Araújo, Thayla S. Maria-Ferreira, Daniele Mendes, Saulo J. F. Ferro, Thiago A. F. Fernandes, Elizabeth S. |
author_facet | Monteiro-Neto, Valério de Souza, Cláudio D. Gonzaga, Laoane F. da Silveira, Bruna C. Sousa, Nágila C. F. Pontes, Jaqueline P. Santos, Deivid M. Martins, Wanessa C. Pessoa, Jorge F. V. Carvalho Júnior, Alexsander R. Almeida, Viviane S. S. de Oliveira, Natália M. T. de Araújo, Thayla S. Maria-Ferreira, Daniele Mendes, Saulo J. F. Ferro, Thiago A. F. Fernandes, Elizabeth S. |
author_sort | Monteiro-Neto, Valério |
collection | PubMed |
description | Escherichia coli and Staphylococcus aureus are important agents of urinary tract infections that can often evolve to severe infections. The rise of antibiotic-resistant strains has driven the search for novel therapies to replace the use or act as adjuvants of antibiotics. In this context, plant-derived compounds have been widely investigated. Cuminaldehyde is suggested as the major antimicrobial compound of the cumin seed essential oil. However, this effect is not fully understood. Herein, we investigated the in silico and in vitro activities of cuminaldehyde, as well as its ability to potentiate ciprofloxacin effects against S. aureus and E. coli. In silico analyses were performed by using different computational tools. The PASS online and SwissADME programmes were used for the prediction of biological activities and oral bioavailability of cuminaldehyde. For analysis of the possible toxic effects and the theoretical pharmacokinetic parameters of the compound, the Osiris, SwissADME and PROTOX programmes were used. Estimations of cuminaldehyde gastrointestinal absorption, blood brain barrier permeability and skin permeation by using SwissADME; and drug likeness and score by using Osiris, were also evaluated The in vitro antimicrobial effects of cuminaldehyde were determined by using microdilution, biofilm formation and time-kill assays. In silico analysis indicated that cuminaldehyde may act as an antimicrobial and as a membrane permeability enhancer. It was suggested to be highly absorbable by the gastrointestinal tract and likely to cross the blood brain barrier. Also, irritative and harmful effects were predicted for cuminaldehyde if swallowed at its LD50. Good oral bioavailability and drug score were also found for this compound. Cuminaldehyde presented antimicrobial and anti-biofilm effects against S. aureus and E. coli.. When co-incubated with ciprofloxacin, it enhanced the antibiotic antimicrobial and anti-biofilm actions. We suggest that cuminaldehyde may be useful as an adjuvant therapy to ciprofloxacin in S. aureus and E. coli-induced infections. |
format | Online Article Text |
id | pubmed-7224478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-72244782020-06-01 Cuminaldehyde potentiates the antimicrobial actions of ciprofloxacin against Staphylococcus aureus and Escherichia coli Monteiro-Neto, Valério de Souza, Cláudio D. Gonzaga, Laoane F. da Silveira, Bruna C. Sousa, Nágila C. F. Pontes, Jaqueline P. Santos, Deivid M. Martins, Wanessa C. Pessoa, Jorge F. V. Carvalho Júnior, Alexsander R. Almeida, Viviane S. S. de Oliveira, Natália M. T. de Araújo, Thayla S. Maria-Ferreira, Daniele Mendes, Saulo J. F. Ferro, Thiago A. F. Fernandes, Elizabeth S. PLoS One Research Article Escherichia coli and Staphylococcus aureus are important agents of urinary tract infections that can often evolve to severe infections. The rise of antibiotic-resistant strains has driven the search for novel therapies to replace the use or act as adjuvants of antibiotics. In this context, plant-derived compounds have been widely investigated. Cuminaldehyde is suggested as the major antimicrobial compound of the cumin seed essential oil. However, this effect is not fully understood. Herein, we investigated the in silico and in vitro activities of cuminaldehyde, as well as its ability to potentiate ciprofloxacin effects against S. aureus and E. coli. In silico analyses were performed by using different computational tools. The PASS online and SwissADME programmes were used for the prediction of biological activities and oral bioavailability of cuminaldehyde. For analysis of the possible toxic effects and the theoretical pharmacokinetic parameters of the compound, the Osiris, SwissADME and PROTOX programmes were used. Estimations of cuminaldehyde gastrointestinal absorption, blood brain barrier permeability and skin permeation by using SwissADME; and drug likeness and score by using Osiris, were also evaluated The in vitro antimicrobial effects of cuminaldehyde were determined by using microdilution, biofilm formation and time-kill assays. In silico analysis indicated that cuminaldehyde may act as an antimicrobial and as a membrane permeability enhancer. It was suggested to be highly absorbable by the gastrointestinal tract and likely to cross the blood brain barrier. Also, irritative and harmful effects were predicted for cuminaldehyde if swallowed at its LD50. Good oral bioavailability and drug score were also found for this compound. Cuminaldehyde presented antimicrobial and anti-biofilm effects against S. aureus and E. coli.. When co-incubated with ciprofloxacin, it enhanced the antibiotic antimicrobial and anti-biofilm actions. We suggest that cuminaldehyde may be useful as an adjuvant therapy to ciprofloxacin in S. aureus and E. coli-induced infections. Public Library of Science 2020-05-14 /pmc/articles/PMC7224478/ /pubmed/32407399 http://dx.doi.org/10.1371/journal.pone.0232987 Text en © 2020 Monteiro-Neto et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Monteiro-Neto, Valério de Souza, Cláudio D. Gonzaga, Laoane F. da Silveira, Bruna C. Sousa, Nágila C. F. Pontes, Jaqueline P. Santos, Deivid M. Martins, Wanessa C. Pessoa, Jorge F. V. Carvalho Júnior, Alexsander R. Almeida, Viviane S. S. de Oliveira, Natália M. T. de Araújo, Thayla S. Maria-Ferreira, Daniele Mendes, Saulo J. F. Ferro, Thiago A. F. Fernandes, Elizabeth S. Cuminaldehyde potentiates the antimicrobial actions of ciprofloxacin against Staphylococcus aureus and Escherichia coli |
title | Cuminaldehyde potentiates the antimicrobial actions of ciprofloxacin against Staphylococcus aureus and Escherichia coli |
title_full | Cuminaldehyde potentiates the antimicrobial actions of ciprofloxacin against Staphylococcus aureus and Escherichia coli |
title_fullStr | Cuminaldehyde potentiates the antimicrobial actions of ciprofloxacin against Staphylococcus aureus and Escherichia coli |
title_full_unstemmed | Cuminaldehyde potentiates the antimicrobial actions of ciprofloxacin against Staphylococcus aureus and Escherichia coli |
title_short | Cuminaldehyde potentiates the antimicrobial actions of ciprofloxacin against Staphylococcus aureus and Escherichia coli |
title_sort | cuminaldehyde potentiates the antimicrobial actions of ciprofloxacin against staphylococcus aureus and escherichia coli |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7224478/ https://www.ncbi.nlm.nih.gov/pubmed/32407399 http://dx.doi.org/10.1371/journal.pone.0232987 |
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