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Arabidopsis Plants Sense Non-self Peptides to Promote Resistance Against Plectosphaerella cucumerina

Peptides are important regulators that participate in the modulation of almost every physiological event in plants, including defense. Recently, many of these peptides have been described as defense elicitors, termed phytocytokines, that are released upon pest or pathogen attack, triggering an ampli...

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Autores principales: Pastor-Fernández, Julia, Gamir, Jordi, Pastor, Victoria, Sanchez-Bel, Paloma, Sanmartín, Neus, Cerezo, Miguel, Flors, Víctor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225342/
https://www.ncbi.nlm.nih.gov/pubmed/32536929
http://dx.doi.org/10.3389/fpls.2020.00529
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author Pastor-Fernández, Julia
Gamir, Jordi
Pastor, Victoria
Sanchez-Bel, Paloma
Sanmartín, Neus
Cerezo, Miguel
Flors, Víctor
author_facet Pastor-Fernández, Julia
Gamir, Jordi
Pastor, Victoria
Sanchez-Bel, Paloma
Sanmartín, Neus
Cerezo, Miguel
Flors, Víctor
author_sort Pastor-Fernández, Julia
collection PubMed
description Peptides are important regulators that participate in the modulation of almost every physiological event in plants, including defense. Recently, many of these peptides have been described as defense elicitors, termed phytocytokines, that are released upon pest or pathogen attack, triggering an amplification of plant defenses. However, little is known about peptides sensing and inducing resistance activities in heterologous plants. In the present study, exogenous peptides from solanaceous species, Systemins and HypSys, are sensed and induce resistance to the necrotrophic fungus Plectosphaerella cucumerina in the taxonomically distant species Arabidopsis thaliana. Surprisingly, other peptides from closer taxonomic clades have very little or no effect on plant protection. In vitro bioassays showed that the studied peptides do not have direct antifungal activities, suggesting that they protect the plant through the promotion of the plant immune system. Interestingly, tomato Systemin was able to induce resistance at very low concentrations (0.1 and 1 nM) and displays a maximum threshold being ineffective above at higher concentrations. Here, we show evidence of the possible involvement of the JA-signaling pathway in the Systemin-Induced Resistance (Sys-IR) in Arabidopsis. Additionally, Systemin treated plants display enhanced BAK1 and BIK1 gene expression following infection as well as increased production of ROS after PAMP treatment suggesting that Systemin sensitizes Arabidopsis perception to pathogens and PAMPs.
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spelling pubmed-72253422020-06-12 Arabidopsis Plants Sense Non-self Peptides to Promote Resistance Against Plectosphaerella cucumerina Pastor-Fernández, Julia Gamir, Jordi Pastor, Victoria Sanchez-Bel, Paloma Sanmartín, Neus Cerezo, Miguel Flors, Víctor Front Plant Sci Plant Science Peptides are important regulators that participate in the modulation of almost every physiological event in plants, including defense. Recently, many of these peptides have been described as defense elicitors, termed phytocytokines, that are released upon pest or pathogen attack, triggering an amplification of plant defenses. However, little is known about peptides sensing and inducing resistance activities in heterologous plants. In the present study, exogenous peptides from solanaceous species, Systemins and HypSys, are sensed and induce resistance to the necrotrophic fungus Plectosphaerella cucumerina in the taxonomically distant species Arabidopsis thaliana. Surprisingly, other peptides from closer taxonomic clades have very little or no effect on plant protection. In vitro bioassays showed that the studied peptides do not have direct antifungal activities, suggesting that they protect the plant through the promotion of the plant immune system. Interestingly, tomato Systemin was able to induce resistance at very low concentrations (0.1 and 1 nM) and displays a maximum threshold being ineffective above at higher concentrations. Here, we show evidence of the possible involvement of the JA-signaling pathway in the Systemin-Induced Resistance (Sys-IR) in Arabidopsis. Additionally, Systemin treated plants display enhanced BAK1 and BIK1 gene expression following infection as well as increased production of ROS after PAMP treatment suggesting that Systemin sensitizes Arabidopsis perception to pathogens and PAMPs. Frontiers Media S.A. 2020-05-08 /pmc/articles/PMC7225342/ /pubmed/32536929 http://dx.doi.org/10.3389/fpls.2020.00529 Text en Copyright © 2020 Pastor-Fernández, Gamir, Pastor, Sanchez-Bel, Sanmartín, Cerezo and Flors. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Pastor-Fernández, Julia
Gamir, Jordi
Pastor, Victoria
Sanchez-Bel, Paloma
Sanmartín, Neus
Cerezo, Miguel
Flors, Víctor
Arabidopsis Plants Sense Non-self Peptides to Promote Resistance Against Plectosphaerella cucumerina
title Arabidopsis Plants Sense Non-self Peptides to Promote Resistance Against Plectosphaerella cucumerina
title_full Arabidopsis Plants Sense Non-self Peptides to Promote Resistance Against Plectosphaerella cucumerina
title_fullStr Arabidopsis Plants Sense Non-self Peptides to Promote Resistance Against Plectosphaerella cucumerina
title_full_unstemmed Arabidopsis Plants Sense Non-self Peptides to Promote Resistance Against Plectosphaerella cucumerina
title_short Arabidopsis Plants Sense Non-self Peptides to Promote Resistance Against Plectosphaerella cucumerina
title_sort arabidopsis plants sense non-self peptides to promote resistance against plectosphaerella cucumerina
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225342/
https://www.ncbi.nlm.nih.gov/pubmed/32536929
http://dx.doi.org/10.3389/fpls.2020.00529
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