Cargando…

Comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in Enterobacterales

OBJECTIVES: Two commercially available lateral flow immunochromatographic assays (ICAs) for detection of the major carbapenemases were prospectively assessed for the detection of carbapenemases in Enterobacterales: RESIST-4 O.K.N.V. (Coris BioConcept) and NG-Test CARBA 5 (NG Biotech). METHODS: These...

Descripción completa

Detalles Bibliográficos
Autores principales: Bogaerts, Pierre, Berger, Anne-Sophie, Evrard, Stéphanie, Huang, Te-Din
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225905/
https://www.ncbi.nlm.nih.gov/pubmed/32083655
http://dx.doi.org/10.1093/jac/dkaa043
_version_ 1783534162588729344
author Bogaerts, Pierre
Berger, Anne-Sophie
Evrard, Stéphanie
Huang, Te-Din
author_facet Bogaerts, Pierre
Berger, Anne-Sophie
Evrard, Stéphanie
Huang, Te-Din
author_sort Bogaerts, Pierre
collection PubMed
description OBJECTIVES: Two commercially available lateral flow immunochromatographic assays (ICAs) for detection of the major carbapenemases were prospectively assessed for the detection of carbapenemases in Enterobacterales: RESIST-4 O.K.N.V. (Coris BioConcept) and NG-Test CARBA 5 (NG Biotech). METHODS: These two assays were performed prospectively on consecutive Enterobacterales suspected of producing a carbapenemase that were referred to the Belgian National Reference Center for Monitoring Antimicrobial Resistance in Gram-Negative Bacteria between March and June 2018. The intensity of the band corresponding to a carbapenemase for each test was compared using ImageJ software. RESULTS: Of the 161 isolates tested, a carbapenemase was detected in 91 (60 OXA-48-like, 15 VIM, 9 KPC, 5 NDM, 1 IMP and 1 IMP + OXA-48); in the remaining 70, no carbapenemases were detected. For both tests, the results were 100% concordant with the results of the PCR-sequencing reference method. Two IMP producers were only detected by NG-Test CARBA 5 as IMP is not targeted by RESIST-4 O.K.N.V. The mean intensity of the OXA-48, VIM and NDM bands displayed by NG-Test CARBA 5 was 3 to 3.7 times higher than for RESIST-4 O.K.N.V., while the KPC band was on average 1.7 times more intense with RESIST-4 O.K.N.V. CONCLUSIONS: RESIST-4 O.K.N.V. and NG-Test CARBA 5 are two efficient assays for identification of the major carbapenemases. NG-Test CARBA 5 offers the advantage of detecting IMP, which remains rare in Western countries.
format Online
Article
Text
id pubmed-7225905
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-72259052020-05-19 Comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in Enterobacterales Bogaerts, Pierre Berger, Anne-Sophie Evrard, Stéphanie Huang, Te-Din J Antimicrob Chemother Original Research OBJECTIVES: Two commercially available lateral flow immunochromatographic assays (ICAs) for detection of the major carbapenemases were prospectively assessed for the detection of carbapenemases in Enterobacterales: RESIST-4 O.K.N.V. (Coris BioConcept) and NG-Test CARBA 5 (NG Biotech). METHODS: These two assays were performed prospectively on consecutive Enterobacterales suspected of producing a carbapenemase that were referred to the Belgian National Reference Center for Monitoring Antimicrobial Resistance in Gram-Negative Bacteria between March and June 2018. The intensity of the band corresponding to a carbapenemase for each test was compared using ImageJ software. RESULTS: Of the 161 isolates tested, a carbapenemase was detected in 91 (60 OXA-48-like, 15 VIM, 9 KPC, 5 NDM, 1 IMP and 1 IMP + OXA-48); in the remaining 70, no carbapenemases were detected. For both tests, the results were 100% concordant with the results of the PCR-sequencing reference method. Two IMP producers were only detected by NG-Test CARBA 5 as IMP is not targeted by RESIST-4 O.K.N.V. The mean intensity of the OXA-48, VIM and NDM bands displayed by NG-Test CARBA 5 was 3 to 3.7 times higher than for RESIST-4 O.K.N.V., while the KPC band was on average 1.7 times more intense with RESIST-4 O.K.N.V. CONCLUSIONS: RESIST-4 O.K.N.V. and NG-Test CARBA 5 are two efficient assays for identification of the major carbapenemases. NG-Test CARBA 5 offers the advantage of detecting IMP, which remains rare in Western countries. Oxford University Press 2020-06 2020-02-21 /pmc/articles/PMC7225905/ /pubmed/32083655 http://dx.doi.org/10.1093/jac/dkaa043 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Original Research
Bogaerts, Pierre
Berger, Anne-Sophie
Evrard, Stéphanie
Huang, Te-Din
Comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in Enterobacterales
title Comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in Enterobacterales
title_full Comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in Enterobacterales
title_fullStr Comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in Enterobacterales
title_full_unstemmed Comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in Enterobacterales
title_short Comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in Enterobacterales
title_sort comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in enterobacterales
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225905/
https://www.ncbi.nlm.nih.gov/pubmed/32083655
http://dx.doi.org/10.1093/jac/dkaa043
work_keys_str_mv AT bogaertspierre comparisonoftwomultipleximmunochromatographicassaysfortherapiddetectionofmajorcarbapenemasesinenterobacterales
AT bergerannesophie comparisonoftwomultipleximmunochromatographicassaysfortherapiddetectionofmajorcarbapenemasesinenterobacterales
AT evrardstephanie comparisonoftwomultipleximmunochromatographicassaysfortherapiddetectionofmajorcarbapenemasesinenterobacterales
AT huangtedin comparisonoftwomultipleximmunochromatographicassaysfortherapiddetectionofmajorcarbapenemasesinenterobacterales