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Expression Pattern and Biological Significance of the lncRNA ST3GAL6-AS1 in Multiple Myeloma
The biological impact of long non-coding RNAs (lncRNAs) in multiple myeloma (MM) is becoming an important aspect of investigation, which may contribute to the understanding of the complex pathobiology of the disease whilst also providing novel potential therapeutic targets. Herein, we investigated t...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225964/ https://www.ncbi.nlm.nih.gov/pubmed/32218309 http://dx.doi.org/10.3390/cancers12040782 |
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author | Ronchetti, Domenica Todoerti, Katia Vinci, Cristina Favasuli, Vanessa Agnelli, Luca Manzoni, Martina Pelizzoni, Francesca Chiaramonte, Raffaella Platonova, Natalia Giuliani, Nicola Tassone, Pierfrancesco Amodio, Nicola Neri, Antonino Taiana, Elisa |
author_facet | Ronchetti, Domenica Todoerti, Katia Vinci, Cristina Favasuli, Vanessa Agnelli, Luca Manzoni, Martina Pelizzoni, Francesca Chiaramonte, Raffaella Platonova, Natalia Giuliani, Nicola Tassone, Pierfrancesco Amodio, Nicola Neri, Antonino Taiana, Elisa |
author_sort | Ronchetti, Domenica |
collection | PubMed |
description | The biological impact of long non-coding RNAs (lncRNAs) in multiple myeloma (MM) is becoming an important aspect of investigation, which may contribute to the understanding of the complex pathobiology of the disease whilst also providing novel potential therapeutic targets. Herein, we investigated the expression pattern and the biological significance of the lncRNA ST3 beta-galactoside alpha-2,3 sialyltransferase 6 antisense RNA 1 (ST3GAL6-AS1) in MM. We documented a high ST3GAL6-AS1 expression level in MM compared to normal plasma cells (PCs) or other hematological malignancies. Transcriptome analyses of MM PCs from patients included in the CoMMpass database indicated a potential involvement of ST3GAL6-AS1 in MAPK signaling and ubiquitin-mediated proteolysis pathways. ST3GAL6-AS1 silencing by LNA-gapmeR antisense oligonucleotides inhibits cell proliferation and triggers apoptosis in MM cell line. Notably, ST3GAL6-AS1 silencing in vitro displayed the down-regulation of the MAPK pathway and protein ubiquitination. These data suggest that ST3GAL6-AS1 deregulation may play a pathogenetic role in MM by affecting both proliferation pathways and circuits fundamental for PC survival. However, ST3GAL6-AS1 expression levels seem not to be significantly associated with clinical outcome and its targeting appears to exert antagonistic effects with proteasome inhibitors used in MM. These findings strongly urge the need for further studies investigating the relevance of ST3GAL6-AS1 in MM. |
format | Online Article Text |
id | pubmed-7225964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72259642020-05-18 Expression Pattern and Biological Significance of the lncRNA ST3GAL6-AS1 in Multiple Myeloma Ronchetti, Domenica Todoerti, Katia Vinci, Cristina Favasuli, Vanessa Agnelli, Luca Manzoni, Martina Pelizzoni, Francesca Chiaramonte, Raffaella Platonova, Natalia Giuliani, Nicola Tassone, Pierfrancesco Amodio, Nicola Neri, Antonino Taiana, Elisa Cancers (Basel) Article The biological impact of long non-coding RNAs (lncRNAs) in multiple myeloma (MM) is becoming an important aspect of investigation, which may contribute to the understanding of the complex pathobiology of the disease whilst also providing novel potential therapeutic targets. Herein, we investigated the expression pattern and the biological significance of the lncRNA ST3 beta-galactoside alpha-2,3 sialyltransferase 6 antisense RNA 1 (ST3GAL6-AS1) in MM. We documented a high ST3GAL6-AS1 expression level in MM compared to normal plasma cells (PCs) or other hematological malignancies. Transcriptome analyses of MM PCs from patients included in the CoMMpass database indicated a potential involvement of ST3GAL6-AS1 in MAPK signaling and ubiquitin-mediated proteolysis pathways. ST3GAL6-AS1 silencing by LNA-gapmeR antisense oligonucleotides inhibits cell proliferation and triggers apoptosis in MM cell line. Notably, ST3GAL6-AS1 silencing in vitro displayed the down-regulation of the MAPK pathway and protein ubiquitination. These data suggest that ST3GAL6-AS1 deregulation may play a pathogenetic role in MM by affecting both proliferation pathways and circuits fundamental for PC survival. However, ST3GAL6-AS1 expression levels seem not to be significantly associated with clinical outcome and its targeting appears to exert antagonistic effects with proteasome inhibitors used in MM. These findings strongly urge the need for further studies investigating the relevance of ST3GAL6-AS1 in MM. MDPI 2020-03-25 /pmc/articles/PMC7225964/ /pubmed/32218309 http://dx.doi.org/10.3390/cancers12040782 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ronchetti, Domenica Todoerti, Katia Vinci, Cristina Favasuli, Vanessa Agnelli, Luca Manzoni, Martina Pelizzoni, Francesca Chiaramonte, Raffaella Platonova, Natalia Giuliani, Nicola Tassone, Pierfrancesco Amodio, Nicola Neri, Antonino Taiana, Elisa Expression Pattern and Biological Significance of the lncRNA ST3GAL6-AS1 in Multiple Myeloma |
title | Expression Pattern and Biological Significance of the lncRNA ST3GAL6-AS1 in Multiple Myeloma |
title_full | Expression Pattern and Biological Significance of the lncRNA ST3GAL6-AS1 in Multiple Myeloma |
title_fullStr | Expression Pattern and Biological Significance of the lncRNA ST3GAL6-AS1 in Multiple Myeloma |
title_full_unstemmed | Expression Pattern and Biological Significance of the lncRNA ST3GAL6-AS1 in Multiple Myeloma |
title_short | Expression Pattern and Biological Significance of the lncRNA ST3GAL6-AS1 in Multiple Myeloma |
title_sort | expression pattern and biological significance of the lncrna st3gal6-as1 in multiple myeloma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225964/ https://www.ncbi.nlm.nih.gov/pubmed/32218309 http://dx.doi.org/10.3390/cancers12040782 |
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