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Evaluation of Pharmacological and Phytochemical Profiles of Piptadeniastrum africanum (Hook.f.) Brenan Stem Bark Extracts

The stem bark (SB) of Piptadeniastrum africanum (PA) has been extensively used in African traditional medicinal systems. However, there is a dearth of scientific information regarding its possible activity in the management of type II diabetes, Alzheimer’s disease, and skin hyperpigmentation disorde...

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Autores principales: Sinan, Kouadio Ibrahime, Chiavaroli, Annalisa, Orlando, Giustino, Bene, Kouadio, Zengin, Gokhan, Cziáky, Zoltán, Jekő, József, Fawzi Mahomoodally, Mohamad, Picot-Allain, Marie Carene Nancy, Menghini, Luigi, Recinella, Lucia, Brunetti, Luigi, Leone, Sheila, Ciferri, Maria Chiara, Di Simone, Simonetta, Ferrante, Claudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226170/
https://www.ncbi.nlm.nih.gov/pubmed/32231150
http://dx.doi.org/10.3390/biom10040516
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author Sinan, Kouadio Ibrahime
Chiavaroli, Annalisa
Orlando, Giustino
Bene, Kouadio
Zengin, Gokhan
Cziáky, Zoltán
Jekő, József
Fawzi Mahomoodally, Mohamad
Picot-Allain, Marie Carene Nancy
Menghini, Luigi
Recinella, Lucia
Brunetti, Luigi
Leone, Sheila
Ciferri, Maria Chiara
Di Simone, Simonetta
Ferrante, Claudio
author_facet Sinan, Kouadio Ibrahime
Chiavaroli, Annalisa
Orlando, Giustino
Bene, Kouadio
Zengin, Gokhan
Cziáky, Zoltán
Jekő, József
Fawzi Mahomoodally, Mohamad
Picot-Allain, Marie Carene Nancy
Menghini, Luigi
Recinella, Lucia
Brunetti, Luigi
Leone, Sheila
Ciferri, Maria Chiara
Di Simone, Simonetta
Ferrante, Claudio
author_sort Sinan, Kouadio Ibrahime
collection PubMed
description The stem bark (SB) of Piptadeniastrum africanum (PA) has been extensively used in African traditional medicinal systems. However, there is a dearth of scientific information regarding its possible activity in the management of type II diabetes, Alzheimer’s disease, and skin hyperpigmentation disorders. This study therefore attempted to elucidate the in vitro inhibitory action of ethyl acetate, methanol, and water extracts of P. africanum stem bark (PA-SB) on α-amylase, α-glucosidase, acetylcholinesterase, butyrylcholinesterase, and tyrosinase. Cell viability, catecholamine, and 3-hydroxykynurenine levels of hypothalamic HypoE22 cells exposed to PA-SB extracts were also investigated. The phytochemical profiles of the extracts were determined by high performance liquid chromatography (HPLC) and antioxidant properties were investigated. Saponin (867.42 mg quillaja equivalent/g) and tannin (33.81 mg catechin equivalent/g) contents were higher in the methanol extract. Multiple dihydroxy-trimethoxy(iso)flavone isomers, loliolide, eriodictyol, naringenin, luteolin, chrysoeriol, apigenin, and liquiritigenin, were characterized from PA-SB extracts using HPLC. The methanol extract of PA-SB showed highest inhibitory activity against acetylcholinesterase (4.88 mg galantamine equivalent (GALAE)/g extract), butyrylcholinesterase (5.37 mg GALAE/g extract), and tyrosinase (154.86 mg kojic acid equivalent/g extract) while α-glucosidase was effectively inhibited by the ethyl acetate extract (15.22 mmol acarbose equivalent/g extract). The methanol extract of PA-SB also showed potent antioxidant properties (493.87, 818.12, 953.07, and 732.19 mg Trolox equivalent/g extract, for 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP) assays, respectively). PA-SB extracts exhibited antioxidant activity and promising inhibition against key enzymes related to type II diabetes, Alzheimer’s disease, and skin hyperpigmentation disorders. Additionally, all extracts were able to contrast hydrogen peroxide-induced oxidative stress, in HypoE22 cells, thus restoring basal catecholamine and 3-hydroxykinurenine levels, whereas only methanol and water extracts stimulated basal dopamine release. Overall, data from the present study contribute to the biological assessment of P. africanum that appears to be a promising source of natural compounds with protective and neuromodulatory effects.
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spelling pubmed-72261702020-05-18 Evaluation of Pharmacological and Phytochemical Profiles of Piptadeniastrum africanum (Hook.f.) Brenan Stem Bark Extracts Sinan, Kouadio Ibrahime Chiavaroli, Annalisa Orlando, Giustino Bene, Kouadio Zengin, Gokhan Cziáky, Zoltán Jekő, József Fawzi Mahomoodally, Mohamad Picot-Allain, Marie Carene Nancy Menghini, Luigi Recinella, Lucia Brunetti, Luigi Leone, Sheila Ciferri, Maria Chiara Di Simone, Simonetta Ferrante, Claudio Biomolecules Article The stem bark (SB) of Piptadeniastrum africanum (PA) has been extensively used in African traditional medicinal systems. However, there is a dearth of scientific information regarding its possible activity in the management of type II diabetes, Alzheimer’s disease, and skin hyperpigmentation disorders. This study therefore attempted to elucidate the in vitro inhibitory action of ethyl acetate, methanol, and water extracts of P. africanum stem bark (PA-SB) on α-amylase, α-glucosidase, acetylcholinesterase, butyrylcholinesterase, and tyrosinase. Cell viability, catecholamine, and 3-hydroxykynurenine levels of hypothalamic HypoE22 cells exposed to PA-SB extracts were also investigated. The phytochemical profiles of the extracts were determined by high performance liquid chromatography (HPLC) and antioxidant properties were investigated. Saponin (867.42 mg quillaja equivalent/g) and tannin (33.81 mg catechin equivalent/g) contents were higher in the methanol extract. Multiple dihydroxy-trimethoxy(iso)flavone isomers, loliolide, eriodictyol, naringenin, luteolin, chrysoeriol, apigenin, and liquiritigenin, were characterized from PA-SB extracts using HPLC. The methanol extract of PA-SB showed highest inhibitory activity against acetylcholinesterase (4.88 mg galantamine equivalent (GALAE)/g extract), butyrylcholinesterase (5.37 mg GALAE/g extract), and tyrosinase (154.86 mg kojic acid equivalent/g extract) while α-glucosidase was effectively inhibited by the ethyl acetate extract (15.22 mmol acarbose equivalent/g extract). The methanol extract of PA-SB also showed potent antioxidant properties (493.87, 818.12, 953.07, and 732.19 mg Trolox equivalent/g extract, for 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP) assays, respectively). PA-SB extracts exhibited antioxidant activity and promising inhibition against key enzymes related to type II diabetes, Alzheimer’s disease, and skin hyperpigmentation disorders. Additionally, all extracts were able to contrast hydrogen peroxide-induced oxidative stress, in HypoE22 cells, thus restoring basal catecholamine and 3-hydroxykinurenine levels, whereas only methanol and water extracts stimulated basal dopamine release. Overall, data from the present study contribute to the biological assessment of P. africanum that appears to be a promising source of natural compounds with protective and neuromodulatory effects. MDPI 2020-03-28 /pmc/articles/PMC7226170/ /pubmed/32231150 http://dx.doi.org/10.3390/biom10040516 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sinan, Kouadio Ibrahime
Chiavaroli, Annalisa
Orlando, Giustino
Bene, Kouadio
Zengin, Gokhan
Cziáky, Zoltán
Jekő, József
Fawzi Mahomoodally, Mohamad
Picot-Allain, Marie Carene Nancy
Menghini, Luigi
Recinella, Lucia
Brunetti, Luigi
Leone, Sheila
Ciferri, Maria Chiara
Di Simone, Simonetta
Ferrante, Claudio
Evaluation of Pharmacological and Phytochemical Profiles of Piptadeniastrum africanum (Hook.f.) Brenan Stem Bark Extracts
title Evaluation of Pharmacological and Phytochemical Profiles of Piptadeniastrum africanum (Hook.f.) Brenan Stem Bark Extracts
title_full Evaluation of Pharmacological and Phytochemical Profiles of Piptadeniastrum africanum (Hook.f.) Brenan Stem Bark Extracts
title_fullStr Evaluation of Pharmacological and Phytochemical Profiles of Piptadeniastrum africanum (Hook.f.) Brenan Stem Bark Extracts
title_full_unstemmed Evaluation of Pharmacological and Phytochemical Profiles of Piptadeniastrum africanum (Hook.f.) Brenan Stem Bark Extracts
title_short Evaluation of Pharmacological and Phytochemical Profiles of Piptadeniastrum africanum (Hook.f.) Brenan Stem Bark Extracts
title_sort evaluation of pharmacological and phytochemical profiles of piptadeniastrum africanum (hook.f.) brenan stem bark extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226170/
https://www.ncbi.nlm.nih.gov/pubmed/32231150
http://dx.doi.org/10.3390/biom10040516
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