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Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison

This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae). Thirty-five secondary metabolites were characterized in...

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Autores principales: Sobeh, Mansour, Rezq, Samar, Cheurfa, Mohammed, Abdelfattah, Mohamed A.O., Rashied, Rasha M.H., El-Shazly, Assem M., Yasri, Abdelaziz, Wink, Michael, Mahmoud, Mona F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226370/
https://www.ncbi.nlm.nih.gov/pubmed/32294957
http://dx.doi.org/10.3390/biom10040599
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author Sobeh, Mansour
Rezq, Samar
Cheurfa, Mohammed
Abdelfattah, Mohamed A.O.
Rashied, Rasha M.H.
El-Shazly, Assem M.
Yasri, Abdelaziz
Wink, Michael
Mahmoud, Mona F.
author_facet Sobeh, Mansour
Rezq, Samar
Cheurfa, Mohammed
Abdelfattah, Mohamed A.O.
Rashied, Rasha M.H.
El-Shazly, Assem M.
Yasri, Abdelaziz
Wink, Michael
Mahmoud, Mona F.
author_sort Sobeh, Mansour
collection PubMed
description This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae). Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract. Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway. In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts. Results revealed that both studied Thymus species exhibited antioxidant, anti-inflammatory, analgesic, and antipyretic effects. They showed to be a more potent antioxidant than ascorbic acid and more selective against cyclooxygenase (COX-2) than diclofenac and indomethacin. Relatively, the T. fontanesii extract was more potent as COX-2 inhibitor than T. algeriensis. In conclusion, Thymus algeriensis and Thymus fontanesii may be interesting candidates for the treatment of inflammation and oxidative stress-related disorders.
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spelling pubmed-72263702020-05-18 Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison Sobeh, Mansour Rezq, Samar Cheurfa, Mohammed Abdelfattah, Mohamed A.O. Rashied, Rasha M.H. El-Shazly, Assem M. Yasri, Abdelaziz Wink, Michael Mahmoud, Mona F. Biomolecules Article This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae). Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract. Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway. In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts. Results revealed that both studied Thymus species exhibited antioxidant, anti-inflammatory, analgesic, and antipyretic effects. They showed to be a more potent antioxidant than ascorbic acid and more selective against cyclooxygenase (COX-2) than diclofenac and indomethacin. Relatively, the T. fontanesii extract was more potent as COX-2 inhibitor than T. algeriensis. In conclusion, Thymus algeriensis and Thymus fontanesii may be interesting candidates for the treatment of inflammation and oxidative stress-related disorders. MDPI 2020-04-13 /pmc/articles/PMC7226370/ /pubmed/32294957 http://dx.doi.org/10.3390/biom10040599 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sobeh, Mansour
Rezq, Samar
Cheurfa, Mohammed
Abdelfattah, Mohamed A.O.
Rashied, Rasha M.H.
El-Shazly, Assem M.
Yasri, Abdelaziz
Wink, Michael
Mahmoud, Mona F.
Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison
title Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison
title_full Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison
title_fullStr Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison
title_full_unstemmed Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison
title_short Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison
title_sort thymus algeriensis and thymus fontanesii: chemical composition, in vivo antiinflammatory, pain killing and antipyretic activities: a comprehensive comparison
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226370/
https://www.ncbi.nlm.nih.gov/pubmed/32294957
http://dx.doi.org/10.3390/biom10040599
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