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Abnormal Reinnervation of Denervated Areas Following Nerve Injury Facilitates Neuropathic Pain

An injury to peripheral nerves leads to skin denervation, which often is followed by increased pain sensitivity of the denervated areas and the development of neuropathic pain. Changes in innervation patterns during the reinnervation process of the denervated skin could contribute to the development...

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Autores principales: Leibovich, Hodaya, Buzaglo, Nahum, Tsuriel, Shlomo, Peretz, Liat, Caspi, Yaki, Katz, Ben, Lev, Shaya, Lichtstein, David, Binshtok, Alexander M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226396/
https://www.ncbi.nlm.nih.gov/pubmed/32325693
http://dx.doi.org/10.3390/cells9041007
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author Leibovich, Hodaya
Buzaglo, Nahum
Tsuriel, Shlomo
Peretz, Liat
Caspi, Yaki
Katz, Ben
Lev, Shaya
Lichtstein, David
Binshtok, Alexander M.
author_facet Leibovich, Hodaya
Buzaglo, Nahum
Tsuriel, Shlomo
Peretz, Liat
Caspi, Yaki
Katz, Ben
Lev, Shaya
Lichtstein, David
Binshtok, Alexander M.
author_sort Leibovich, Hodaya
collection PubMed
description An injury to peripheral nerves leads to skin denervation, which often is followed by increased pain sensitivity of the denervated areas and the development of neuropathic pain. Changes in innervation patterns during the reinnervation process of the denervated skin could contribute to the development of neuropathic pain. Here, we examined the changes in the innervation pattern during reinnervation and correlated them with the symptoms of neuropathic pain. Using a multispectral labeling technique—PainBow, which we developed, we characterized dorsal root ganglion (DRG) neurons innervating distinct areas of the rats’ paw. We then used spared nerve injury, causing partial denervation of the paw, and examined the changes in innervation patterns of the denervated areas during the development of allodynia and hyperalgesia. We found that, differently from normal conditions, during the development of neuropathic pain, these areas were mainly innervated by large, non-nociceptive neurons. Moreover, we found that the development of neuropathic pain is correlated with an overall decrease in the number of DRG neurons innervating these areas. Importantly, treatment with ouabain facilitated reinnervation and alleviated neuropathic pain. Our results suggest that local changes in peripheral innervation following denervation contribute to neuropathic pain development. The reversal of these changes decreases neuropathic pain.
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spelling pubmed-72263962020-05-18 Abnormal Reinnervation of Denervated Areas Following Nerve Injury Facilitates Neuropathic Pain Leibovich, Hodaya Buzaglo, Nahum Tsuriel, Shlomo Peretz, Liat Caspi, Yaki Katz, Ben Lev, Shaya Lichtstein, David Binshtok, Alexander M. Cells Article An injury to peripheral nerves leads to skin denervation, which often is followed by increased pain sensitivity of the denervated areas and the development of neuropathic pain. Changes in innervation patterns during the reinnervation process of the denervated skin could contribute to the development of neuropathic pain. Here, we examined the changes in the innervation pattern during reinnervation and correlated them with the symptoms of neuropathic pain. Using a multispectral labeling technique—PainBow, which we developed, we characterized dorsal root ganglion (DRG) neurons innervating distinct areas of the rats’ paw. We then used spared nerve injury, causing partial denervation of the paw, and examined the changes in innervation patterns of the denervated areas during the development of allodynia and hyperalgesia. We found that, differently from normal conditions, during the development of neuropathic pain, these areas were mainly innervated by large, non-nociceptive neurons. Moreover, we found that the development of neuropathic pain is correlated with an overall decrease in the number of DRG neurons innervating these areas. Importantly, treatment with ouabain facilitated reinnervation and alleviated neuropathic pain. Our results suggest that local changes in peripheral innervation following denervation contribute to neuropathic pain development. The reversal of these changes decreases neuropathic pain. MDPI 2020-04-18 /pmc/articles/PMC7226396/ /pubmed/32325693 http://dx.doi.org/10.3390/cells9041007 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Leibovich, Hodaya
Buzaglo, Nahum
Tsuriel, Shlomo
Peretz, Liat
Caspi, Yaki
Katz, Ben
Lev, Shaya
Lichtstein, David
Binshtok, Alexander M.
Abnormal Reinnervation of Denervated Areas Following Nerve Injury Facilitates Neuropathic Pain
title Abnormal Reinnervation of Denervated Areas Following Nerve Injury Facilitates Neuropathic Pain
title_full Abnormal Reinnervation of Denervated Areas Following Nerve Injury Facilitates Neuropathic Pain
title_fullStr Abnormal Reinnervation of Denervated Areas Following Nerve Injury Facilitates Neuropathic Pain
title_full_unstemmed Abnormal Reinnervation of Denervated Areas Following Nerve Injury Facilitates Neuropathic Pain
title_short Abnormal Reinnervation of Denervated Areas Following Nerve Injury Facilitates Neuropathic Pain
title_sort abnormal reinnervation of denervated areas following nerve injury facilitates neuropathic pain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226396/
https://www.ncbi.nlm.nih.gov/pubmed/32325693
http://dx.doi.org/10.3390/cells9041007
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