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Changes in the Th9 cell population and related cytokines in the peripheral blood of infants with recurrent wheezing

INTRODUCTION: T helper type 9 (Th9) cells have been shown to play a key role in initiating allergic reactions and promoting airway inflammation. However, to the best of our knowledge, their role has not been analyzed in infants with recurrent wheezing. MATERIAL AND METHODS: We performed a case-contr...

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Autores principales: LIU, GUANGLIANG, QIAN, LEI, XU, TONGHUI, YU, JIANXIU, LI, MING, CUI, YUBAO
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226556/
https://www.ncbi.nlm.nih.gov/pubmed/32425681
http://dx.doi.org/10.5114/ceji.2020.94683
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author LIU, GUANGLIANG
QIAN, LEI
XU, TONGHUI
YU, JIANXIU
LI, MING
CUI, YUBAO
author_facet LIU, GUANGLIANG
QIAN, LEI
XU, TONGHUI
YU, JIANXIU
LI, MING
CUI, YUBAO
author_sort LIU, GUANGLIANG
collection PubMed
description INTRODUCTION: T helper type 9 (Th9) cells have been shown to play a key role in initiating allergic reactions and promoting airway inflammation. However, to the best of our knowledge, their role has not been analyzed in infants with recurrent wheezing. MATERIAL AND METHODS: We performed a case-control study including 34 infants with recurrent wheezing and the same number of healthy infants as controls; all subjects were aged 1- to 3-years-old. The Th9 cell populations in the peripheral blood of these subjects were analyzed using flow cytometry, along with the assessment of Th9- and Th2-related plasma cytokine levels, including interleukin (IL)-1β, IL-4, IL-5, IL-9, IL-10, IL-13, IL-17A, and IL-33, and transforming growth factor β1 (TGF-β1) using a Luminex 200 immunoassay. RESULTS: Our results indicatedthat infants with recurrent wheezing had higher percentages of Th9 cells (median, 0.69%; range, 0.46-1.08%) as compared to healthy infants (median, 0.25%, range, 0.13-0.36%; p < 0.05). In addition, infants with recurrent wheezing also exhibited higher plasma levels of cytokines IL-4, IL-9, IL-10, IL-33, and TGF-β1. Furthermore, the percentage of Th9 cells was positively correlated with the levels of IL-4 (r = 0.408, p < 0.05) and IL-9 (r = 0.644, p < 0.05) in the peripheral blood of wheezing infants. CONCLUSIONS: Our findings suggest that the percentage of Th9 cells is increased in infants with recurrent wheezing; thus, Th9 cells may play an important role in the pathogenesis of recurrent wheezing.
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spelling pubmed-72265562020-05-18 Changes in the Th9 cell population and related cytokines in the peripheral blood of infants with recurrent wheezing LIU, GUANGLIANG QIAN, LEI XU, TONGHUI YU, JIANXIU LI, MING CUI, YUBAO Cent Eur J Immunol Clinical Immunology INTRODUCTION: T helper type 9 (Th9) cells have been shown to play a key role in initiating allergic reactions and promoting airway inflammation. However, to the best of our knowledge, their role has not been analyzed in infants with recurrent wheezing. MATERIAL AND METHODS: We performed a case-control study including 34 infants with recurrent wheezing and the same number of healthy infants as controls; all subjects were aged 1- to 3-years-old. The Th9 cell populations in the peripheral blood of these subjects were analyzed using flow cytometry, along with the assessment of Th9- and Th2-related plasma cytokine levels, including interleukin (IL)-1β, IL-4, IL-5, IL-9, IL-10, IL-13, IL-17A, and IL-33, and transforming growth factor β1 (TGF-β1) using a Luminex 200 immunoassay. RESULTS: Our results indicatedthat infants with recurrent wheezing had higher percentages of Th9 cells (median, 0.69%; range, 0.46-1.08%) as compared to healthy infants (median, 0.25%, range, 0.13-0.36%; p < 0.05). In addition, infants with recurrent wheezing also exhibited higher plasma levels of cytokines IL-4, IL-9, IL-10, IL-33, and TGF-β1. Furthermore, the percentage of Th9 cells was positively correlated with the levels of IL-4 (r = 0.408, p < 0.05) and IL-9 (r = 0.644, p < 0.05) in the peripheral blood of wheezing infants. CONCLUSIONS: Our findings suggest that the percentage of Th9 cells is increased in infants with recurrent wheezing; thus, Th9 cells may play an important role in the pathogenesis of recurrent wheezing. Termedia Publishing House 2020-04 2020 /pmc/articles/PMC7226556/ /pubmed/32425681 http://dx.doi.org/10.5114/ceji.2020.94683 Text en Copyright © 2020 Termedia http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). License (http://creativecommons.org/licenses/by-nc-sa/4.0/)
spellingShingle Clinical Immunology
LIU, GUANGLIANG
QIAN, LEI
XU, TONGHUI
YU, JIANXIU
LI, MING
CUI, YUBAO
Changes in the Th9 cell population and related cytokines in the peripheral blood of infants with recurrent wheezing
title Changes in the Th9 cell population and related cytokines in the peripheral blood of infants with recurrent wheezing
title_full Changes in the Th9 cell population and related cytokines in the peripheral blood of infants with recurrent wheezing
title_fullStr Changes in the Th9 cell population and related cytokines in the peripheral blood of infants with recurrent wheezing
title_full_unstemmed Changes in the Th9 cell population and related cytokines in the peripheral blood of infants with recurrent wheezing
title_short Changes in the Th9 cell population and related cytokines in the peripheral blood of infants with recurrent wheezing
title_sort changes in the th9 cell population and related cytokines in the peripheral blood of infants with recurrent wheezing
topic Clinical Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226556/
https://www.ncbi.nlm.nih.gov/pubmed/32425681
http://dx.doi.org/10.5114/ceji.2020.94683
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