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Comparison of Aneurysm Patency and Mural Inflammation in an Arterial Rabbit Sidewall and Bifurcation Aneurysm Model under Consideration of Different Wall Conditions

Background: Biological processes that lead to aneurysm formation, growth and rupture are insufficiently understood. Vessel wall inflammation and degeneration are suggested to be the driving factors. In this study, we aimed to investigate the natural course of vital (non-decellularized) and decellula...

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Autores principales: Grüter, Basil Erwin, Wanderer, Stefan, Strange, Fabio, Sivanrupan, Sivani, von Gunten, Michael, Widmer, Hans Rudolf, Coluccia, Daniel, Andereggen, Lukas, Fandino, Javier, Marbacher, Serge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226569/
https://www.ncbi.nlm.nih.gov/pubmed/32230757
http://dx.doi.org/10.3390/brainsci10040197
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author Grüter, Basil Erwin
Wanderer, Stefan
Strange, Fabio
Sivanrupan, Sivani
von Gunten, Michael
Widmer, Hans Rudolf
Coluccia, Daniel
Andereggen, Lukas
Fandino, Javier
Marbacher, Serge
author_facet Grüter, Basil Erwin
Wanderer, Stefan
Strange, Fabio
Sivanrupan, Sivani
von Gunten, Michael
Widmer, Hans Rudolf
Coluccia, Daniel
Andereggen, Lukas
Fandino, Javier
Marbacher, Serge
author_sort Grüter, Basil Erwin
collection PubMed
description Background: Biological processes that lead to aneurysm formation, growth and rupture are insufficiently understood. Vessel wall inflammation and degeneration are suggested to be the driving factors. In this study, we aimed to investigate the natural course of vital (non-decellularized) and decellularized aneurysms in a rabbit sidewall and bifurcation model. Methods: Arterial pouches were sutured end-to-side on the carotid artery of New Zealand White rabbits (vital [n = 6] or decellularized [n = 6]), and into an end-to-side common carotid artery bifurcation (vital [n = 6] and decellularized [n = 6]). Patency was confirmed by fluorescence angiography. After 28 days, all animals underwent magnetic resonance and fluorescence angiography followed by aneurysm harvesting for macroscopic and histological evaluation. Results: None of the aneurysms ruptured during follow-up. All sidewall aneurysms thrombosed with histological inferior thrombus organization observed in decellularized compared to vital aneurysms. In the bifurcation model, half of all decellularized aneurysms thrombosed whereas the non-decellularized aneurysms remained patent with relevant increase in size compared to baseline. Conclusions: Poor thrombus organization in decellularized sidewall aneurysms confirmed the important role of mural cells in aneurysm healing after thrombus formation. Several factors such as restriction by neck tissue, small dimensions and hemodynamics may have prevented aneurysm growth despite pronounced inflammation in decellularized aneurysms. In the bifurcation model, rarefication of mural cells did not increase the risk of aneurysm growth but tendency to spontaneous thrombosis.
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spelling pubmed-72265692020-05-18 Comparison of Aneurysm Patency and Mural Inflammation in an Arterial Rabbit Sidewall and Bifurcation Aneurysm Model under Consideration of Different Wall Conditions Grüter, Basil Erwin Wanderer, Stefan Strange, Fabio Sivanrupan, Sivani von Gunten, Michael Widmer, Hans Rudolf Coluccia, Daniel Andereggen, Lukas Fandino, Javier Marbacher, Serge Brain Sci Article Background: Biological processes that lead to aneurysm formation, growth and rupture are insufficiently understood. Vessel wall inflammation and degeneration are suggested to be the driving factors. In this study, we aimed to investigate the natural course of vital (non-decellularized) and decellularized aneurysms in a rabbit sidewall and bifurcation model. Methods: Arterial pouches were sutured end-to-side on the carotid artery of New Zealand White rabbits (vital [n = 6] or decellularized [n = 6]), and into an end-to-side common carotid artery bifurcation (vital [n = 6] and decellularized [n = 6]). Patency was confirmed by fluorescence angiography. After 28 days, all animals underwent magnetic resonance and fluorescence angiography followed by aneurysm harvesting for macroscopic and histological evaluation. Results: None of the aneurysms ruptured during follow-up. All sidewall aneurysms thrombosed with histological inferior thrombus organization observed in decellularized compared to vital aneurysms. In the bifurcation model, half of all decellularized aneurysms thrombosed whereas the non-decellularized aneurysms remained patent with relevant increase in size compared to baseline. Conclusions: Poor thrombus organization in decellularized sidewall aneurysms confirmed the important role of mural cells in aneurysm healing after thrombus formation. Several factors such as restriction by neck tissue, small dimensions and hemodynamics may have prevented aneurysm growth despite pronounced inflammation in decellularized aneurysms. In the bifurcation model, rarefication of mural cells did not increase the risk of aneurysm growth but tendency to spontaneous thrombosis. MDPI 2020-03-27 /pmc/articles/PMC7226569/ /pubmed/32230757 http://dx.doi.org/10.3390/brainsci10040197 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grüter, Basil Erwin
Wanderer, Stefan
Strange, Fabio
Sivanrupan, Sivani
von Gunten, Michael
Widmer, Hans Rudolf
Coluccia, Daniel
Andereggen, Lukas
Fandino, Javier
Marbacher, Serge
Comparison of Aneurysm Patency and Mural Inflammation in an Arterial Rabbit Sidewall and Bifurcation Aneurysm Model under Consideration of Different Wall Conditions
title Comparison of Aneurysm Patency and Mural Inflammation in an Arterial Rabbit Sidewall and Bifurcation Aneurysm Model under Consideration of Different Wall Conditions
title_full Comparison of Aneurysm Patency and Mural Inflammation in an Arterial Rabbit Sidewall and Bifurcation Aneurysm Model under Consideration of Different Wall Conditions
title_fullStr Comparison of Aneurysm Patency and Mural Inflammation in an Arterial Rabbit Sidewall and Bifurcation Aneurysm Model under Consideration of Different Wall Conditions
title_full_unstemmed Comparison of Aneurysm Patency and Mural Inflammation in an Arterial Rabbit Sidewall and Bifurcation Aneurysm Model under Consideration of Different Wall Conditions
title_short Comparison of Aneurysm Patency and Mural Inflammation in an Arterial Rabbit Sidewall and Bifurcation Aneurysm Model under Consideration of Different Wall Conditions
title_sort comparison of aneurysm patency and mural inflammation in an arterial rabbit sidewall and bifurcation aneurysm model under consideration of different wall conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226569/
https://www.ncbi.nlm.nih.gov/pubmed/32230757
http://dx.doi.org/10.3390/brainsci10040197
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