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A Novel Approach for Quantifying Cancer Cells Showing Hybrid Epithelial/Mesenchymal States in Large Series of Tissue Samples: Towards a New Prognostic Marker

In cancer biology, epithelial-to-mesenchymal transition (EMT) is associated with tumorigenesis, stemness, invasion, metastasis, and resistance to therapy. Evidence of co-expression of epithelial and mesenchymal markers suggests that EMT should be a stepwise process with distinct intermediate states...

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Autores principales: Godin, Louis, Balsat, Cédric, Van Eycke, Yves-Rémi, Allard, Justine, Royer, Claire, Remmelink, Myriam, Pastushenko, Ievgenia, D’Haene, Nicky, Blanpain, Cédric, Salmon, Isabelle, Rorive, Sandrine, Decaestecker, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226581/
https://www.ncbi.nlm.nih.gov/pubmed/32276404
http://dx.doi.org/10.3390/cancers12040906
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author Godin, Louis
Balsat, Cédric
Van Eycke, Yves-Rémi
Allard, Justine
Royer, Claire
Remmelink, Myriam
Pastushenko, Ievgenia
D’Haene, Nicky
Blanpain, Cédric
Salmon, Isabelle
Rorive, Sandrine
Decaestecker, Christine
author_facet Godin, Louis
Balsat, Cédric
Van Eycke, Yves-Rémi
Allard, Justine
Royer, Claire
Remmelink, Myriam
Pastushenko, Ievgenia
D’Haene, Nicky
Blanpain, Cédric
Salmon, Isabelle
Rorive, Sandrine
Decaestecker, Christine
author_sort Godin, Louis
collection PubMed
description In cancer biology, epithelial-to-mesenchymal transition (EMT) is associated with tumorigenesis, stemness, invasion, metastasis, and resistance to therapy. Evidence of co-expression of epithelial and mesenchymal markers suggests that EMT should be a stepwise process with distinct intermediate states rather than a binary switch. In the present study, we propose a morphological approach that enables the detection and quantification of cancer cells with hybrid E/M states, i.e., which combine partially epithelial (E) and partially mesenchymal (M) states. This approach is based on a sequential immunohistochemistry technique performed on the same tissue section, the digitization of whole slides, and image processing. The aim is to extract quantitative indicators able to quantify the presence of hybrid E/M states in large series of human cancer samples and to analyze their relationship with cancer aggressiveness. As a proof of concept, we applied our methodology to a series of about a hundred urothelial carcinomas and demonstrated that the presence of cancer cells with hybrid E/M phenotypes at the time of diagnosis is strongly associated with a poor prognostic value, independently of standard clinicopathological features. Although validation on a larger case series and other cancer types is required, our data support the hybrid E/M score as a promising prognostic biomarker for carcinoma patients.
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spelling pubmed-72265812020-05-18 A Novel Approach for Quantifying Cancer Cells Showing Hybrid Epithelial/Mesenchymal States in Large Series of Tissue Samples: Towards a New Prognostic Marker Godin, Louis Balsat, Cédric Van Eycke, Yves-Rémi Allard, Justine Royer, Claire Remmelink, Myriam Pastushenko, Ievgenia D’Haene, Nicky Blanpain, Cédric Salmon, Isabelle Rorive, Sandrine Decaestecker, Christine Cancers (Basel) Article In cancer biology, epithelial-to-mesenchymal transition (EMT) is associated with tumorigenesis, stemness, invasion, metastasis, and resistance to therapy. Evidence of co-expression of epithelial and mesenchymal markers suggests that EMT should be a stepwise process with distinct intermediate states rather than a binary switch. In the present study, we propose a morphological approach that enables the detection and quantification of cancer cells with hybrid E/M states, i.e., which combine partially epithelial (E) and partially mesenchymal (M) states. This approach is based on a sequential immunohistochemistry technique performed on the same tissue section, the digitization of whole slides, and image processing. The aim is to extract quantitative indicators able to quantify the presence of hybrid E/M states in large series of human cancer samples and to analyze their relationship with cancer aggressiveness. As a proof of concept, we applied our methodology to a series of about a hundred urothelial carcinomas and demonstrated that the presence of cancer cells with hybrid E/M phenotypes at the time of diagnosis is strongly associated with a poor prognostic value, independently of standard clinicopathological features. Although validation on a larger case series and other cancer types is required, our data support the hybrid E/M score as a promising prognostic biomarker for carcinoma patients. MDPI 2020-04-08 /pmc/articles/PMC7226581/ /pubmed/32276404 http://dx.doi.org/10.3390/cancers12040906 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Godin, Louis
Balsat, Cédric
Van Eycke, Yves-Rémi
Allard, Justine
Royer, Claire
Remmelink, Myriam
Pastushenko, Ievgenia
D’Haene, Nicky
Blanpain, Cédric
Salmon, Isabelle
Rorive, Sandrine
Decaestecker, Christine
A Novel Approach for Quantifying Cancer Cells Showing Hybrid Epithelial/Mesenchymal States in Large Series of Tissue Samples: Towards a New Prognostic Marker
title A Novel Approach for Quantifying Cancer Cells Showing Hybrid Epithelial/Mesenchymal States in Large Series of Tissue Samples: Towards a New Prognostic Marker
title_full A Novel Approach for Quantifying Cancer Cells Showing Hybrid Epithelial/Mesenchymal States in Large Series of Tissue Samples: Towards a New Prognostic Marker
title_fullStr A Novel Approach for Quantifying Cancer Cells Showing Hybrid Epithelial/Mesenchymal States in Large Series of Tissue Samples: Towards a New Prognostic Marker
title_full_unstemmed A Novel Approach for Quantifying Cancer Cells Showing Hybrid Epithelial/Mesenchymal States in Large Series of Tissue Samples: Towards a New Prognostic Marker
title_short A Novel Approach for Quantifying Cancer Cells Showing Hybrid Epithelial/Mesenchymal States in Large Series of Tissue Samples: Towards a New Prognostic Marker
title_sort novel approach for quantifying cancer cells showing hybrid epithelial/mesenchymal states in large series of tissue samples: towards a new prognostic marker
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226581/
https://www.ncbi.nlm.nih.gov/pubmed/32276404
http://dx.doi.org/10.3390/cancers12040906
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