Construction of a Bacillus subtilis and Escherichia coli shuttle vector harboring the fabL gene as a triclosan selection marker

A new plasmid containing a mutated fabL gene from Bacillus subtilis as a triclosan selection marker was developed as a useful B. subtilis/E. coli shuttle vector. The pHT-FabL40 plasmid is stable in both gram-positive and gram-negative hosts with increased plasmid DNA yield in E. coli.

Detalles Bibliográficos
Autores principales: Mordukhova, Elena A., Pan, Jae-Gu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226672/
https://www.ncbi.nlm.nih.gov/pubmed/32426536
http://dx.doi.org/10.1016/j.heliyon.2020.e03891
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author Mordukhova, Elena A.
Pan, Jae-Gu
author_facet Mordukhova, Elena A.
Pan, Jae-Gu
author_sort Mordukhova, Elena A.
collection PubMed
description A new plasmid containing a mutated fabL gene from Bacillus subtilis as a triclosan selection marker was developed as a useful B. subtilis/E. coli shuttle vector. The pHT-FabL40 plasmid is stable in both gram-positive and gram-negative hosts with increased plasmid DNA yield in E. coli.
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spelling pubmed-72266722020-05-18 Construction of a Bacillus subtilis and Escherichia coli shuttle vector harboring the fabL gene as a triclosan selection marker Mordukhova, Elena A. Pan, Jae-Gu Heliyon Article A new plasmid containing a mutated fabL gene from Bacillus subtilis as a triclosan selection marker was developed as a useful B. subtilis/E. coli shuttle vector. The pHT-FabL40 plasmid is stable in both gram-positive and gram-negative hosts with increased plasmid DNA yield in E. coli. Elsevier 2020-05-13 /pmc/articles/PMC7226672/ /pubmed/32426536 http://dx.doi.org/10.1016/j.heliyon.2020.e03891 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mordukhova, Elena A.
Pan, Jae-Gu
Construction of a Bacillus subtilis and Escherichia coli shuttle vector harboring the fabL gene as a triclosan selection marker
title Construction of a Bacillus subtilis and Escherichia coli shuttle vector harboring the fabL gene as a triclosan selection marker
title_full Construction of a Bacillus subtilis and Escherichia coli shuttle vector harboring the fabL gene as a triclosan selection marker
title_fullStr Construction of a Bacillus subtilis and Escherichia coli shuttle vector harboring the fabL gene as a triclosan selection marker
title_full_unstemmed Construction of a Bacillus subtilis and Escherichia coli shuttle vector harboring the fabL gene as a triclosan selection marker
title_short Construction of a Bacillus subtilis and Escherichia coli shuttle vector harboring the fabL gene as a triclosan selection marker
title_sort construction of a bacillus subtilis and escherichia coli shuttle vector harboring the fabl gene as a triclosan selection marker
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226672/
https://www.ncbi.nlm.nih.gov/pubmed/32426536
http://dx.doi.org/10.1016/j.heliyon.2020.e03891
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