A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos

The sea urchin embryo provides a valuable system to analyse the molecular mechanisms orchestrating cell cycle progression and mitosis in a developmental context. However, although it is known that the regulation of histone activity by post-translational modification plays an important role during ce...

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Autores principales: Feizbakhsh, Omid, Pontheaux, Florian, Glippa, Virginie, Morales, Julia, Ruchaud, Sandrine, Cormier, Patrick, Roch, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226724/
https://www.ncbi.nlm.nih.gov/pubmed/32272587
http://dx.doi.org/10.3390/cells9040898
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author Feizbakhsh, Omid
Pontheaux, Florian
Glippa, Virginie
Morales, Julia
Ruchaud, Sandrine
Cormier, Patrick
Roch, Fernando
author_facet Feizbakhsh, Omid
Pontheaux, Florian
Glippa, Virginie
Morales, Julia
Ruchaud, Sandrine
Cormier, Patrick
Roch, Fernando
author_sort Feizbakhsh, Omid
collection PubMed
description The sea urchin embryo provides a valuable system to analyse the molecular mechanisms orchestrating cell cycle progression and mitosis in a developmental context. However, although it is known that the regulation of histone activity by post-translational modification plays an important role during cell division, the dynamics and the impact of these modifications have not been characterised in detail in a developing embryo. Using different immuno-detection techniques, we show that the levels of Histone 3 phosphorylation at Threonine 3 oscillate in synchrony with mitosis in Sphaerechinus granularis early embryos. We present, in addition, the results of a pharmacological study aimed at analysing the role of this key histone post-translational modification during sea urchin early development.
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spelling pubmed-72267242020-05-18 A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos Feizbakhsh, Omid Pontheaux, Florian Glippa, Virginie Morales, Julia Ruchaud, Sandrine Cormier, Patrick Roch, Fernando Cells Article The sea urchin embryo provides a valuable system to analyse the molecular mechanisms orchestrating cell cycle progression and mitosis in a developmental context. However, although it is known that the regulation of histone activity by post-translational modification plays an important role during cell division, the dynamics and the impact of these modifications have not been characterised in detail in a developing embryo. Using different immuno-detection techniques, we show that the levels of Histone 3 phosphorylation at Threonine 3 oscillate in synchrony with mitosis in Sphaerechinus granularis early embryos. We present, in addition, the results of a pharmacological study aimed at analysing the role of this key histone post-translational modification during sea urchin early development. MDPI 2020-04-07 /pmc/articles/PMC7226724/ /pubmed/32272587 http://dx.doi.org/10.3390/cells9040898 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Feizbakhsh, Omid
Pontheaux, Florian
Glippa, Virginie
Morales, Julia
Ruchaud, Sandrine
Cormier, Patrick
Roch, Fernando
A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos
title A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos
title_full A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos
title_fullStr A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos
title_full_unstemmed A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos
title_short A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos
title_sort peak of h3t3 phosphorylation occurs in synchrony with mitosis in sea urchin early embryos
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226724/
https://www.ncbi.nlm.nih.gov/pubmed/32272587
http://dx.doi.org/10.3390/cells9040898
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