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CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice

There are over 200 genes that are predicted to be solely expressed in the oocyte and ovary, and thousands more that have expression patterns in the female reproductive tract. Unfortunately, many of their physiological functions, such as their roles in oogenesis or fertilization, have yet to be eluci...

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Autores principales: Abbasi, Ferheen, Kodani, Mayo, Emori, Chihiro, Kiyozumi, Daiji, Mori, Masashi, Fujihara, Yoshitaka, Ikawa, Masahito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226750/
https://www.ncbi.nlm.nih.gov/pubmed/32231122
http://dx.doi.org/10.3390/cells9040821
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author Abbasi, Ferheen
Kodani, Mayo
Emori, Chihiro
Kiyozumi, Daiji
Mori, Masashi
Fujihara, Yoshitaka
Ikawa, Masahito
author_facet Abbasi, Ferheen
Kodani, Mayo
Emori, Chihiro
Kiyozumi, Daiji
Mori, Masashi
Fujihara, Yoshitaka
Ikawa, Masahito
author_sort Abbasi, Ferheen
collection PubMed
description There are over 200 genes that are predicted to be solely expressed in the oocyte and ovary, and thousands more that have expression patterns in the female reproductive tract. Unfortunately, many of their physiological functions, such as their roles in oogenesis or fertilization, have yet to be elucidated. Previous knockout (KO) mice studies have proven that many of the genes that were once thought to be essential for fertility are dispensable in vivo. Therefore, it is extremely important to confirm the roles of all genes before spending immense time studying them in vitro. To do this, our laboratory analyzes the functions of ovary and oocyte-enriched genes in vivo through generating CRISPR/Cas9 KO mice and examining their fertility. In this study, we have knocked out three Oosp family genes (Oosp1, Oosp2, and Oosp3) that have expression patterns linked to the female reproductive system and found that the triple KO (TKO) mutant mice generated exhibited decreased prolificacy but were not infertile; thus, these genes may potentially be dispensable for fertility. We also generated Cd160 and Egfl6 KO mice and found these genes are individually dispensable for female fertility. KO mice with no phenotypic data are seldom published, but we believe that this information must be shared to prevent unnecessary experimentation by other laboratories.
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spelling pubmed-72267502020-05-18 CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice Abbasi, Ferheen Kodani, Mayo Emori, Chihiro Kiyozumi, Daiji Mori, Masashi Fujihara, Yoshitaka Ikawa, Masahito Cells Article There are over 200 genes that are predicted to be solely expressed in the oocyte and ovary, and thousands more that have expression patterns in the female reproductive tract. Unfortunately, many of their physiological functions, such as their roles in oogenesis or fertilization, have yet to be elucidated. Previous knockout (KO) mice studies have proven that many of the genes that were once thought to be essential for fertility are dispensable in vivo. Therefore, it is extremely important to confirm the roles of all genes before spending immense time studying them in vitro. To do this, our laboratory analyzes the functions of ovary and oocyte-enriched genes in vivo through generating CRISPR/Cas9 KO mice and examining their fertility. In this study, we have knocked out three Oosp family genes (Oosp1, Oosp2, and Oosp3) that have expression patterns linked to the female reproductive system and found that the triple KO (TKO) mutant mice generated exhibited decreased prolificacy but were not infertile; thus, these genes may potentially be dispensable for fertility. We also generated Cd160 and Egfl6 KO mice and found these genes are individually dispensable for female fertility. KO mice with no phenotypic data are seldom published, but we believe that this information must be shared to prevent unnecessary experimentation by other laboratories. MDPI 2020-03-28 /pmc/articles/PMC7226750/ /pubmed/32231122 http://dx.doi.org/10.3390/cells9040821 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abbasi, Ferheen
Kodani, Mayo
Emori, Chihiro
Kiyozumi, Daiji
Mori, Masashi
Fujihara, Yoshitaka
Ikawa, Masahito
CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice
title CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice
title_full CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice
title_fullStr CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice
title_full_unstemmed CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice
title_short CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice
title_sort crispr/cas9-mediated genome editing reveals oosp family genes are dispensable for female fertility in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226750/
https://www.ncbi.nlm.nih.gov/pubmed/32231122
http://dx.doi.org/10.3390/cells9040821
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