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Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells
Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key can...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226778/ https://www.ncbi.nlm.nih.gov/pubmed/32276503 http://dx.doi.org/10.3390/cells9040914 |
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author | De Kock, Joery Rodrigues, Robim Marcelino Branson, Steven Verhoye, Lieven Colemonts-Vroninks, Haaike Rombaut, Matthias Boeckmans, Joost Neuckermans, Jessie Lequeue, Sien Buyl, Karolien Merimi, Makram Agha, Douaa Moussa De Boe, Veerle Lagneaux, Laurence Meuleman, Philip Vanhaecke, Tamara Najar, Mehdi |
author_facet | De Kock, Joery Rodrigues, Robim Marcelino Branson, Steven Verhoye, Lieven Colemonts-Vroninks, Haaike Rombaut, Matthias Boeckmans, Joost Neuckermans, Jessie Lequeue, Sien Buyl, Karolien Merimi, Makram Agha, Douaa Moussa De Boe, Veerle Lagneaux, Laurence Meuleman, Philip Vanhaecke, Tamara Najar, Mehdi |
author_sort | De Kock, Joery |
collection | PubMed |
description | Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies. |
format | Online Article Text |
id | pubmed-7226778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72267782020-05-18 Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells De Kock, Joery Rodrigues, Robim Marcelino Branson, Steven Verhoye, Lieven Colemonts-Vroninks, Haaike Rombaut, Matthias Boeckmans, Joost Neuckermans, Jessie Lequeue, Sien Buyl, Karolien Merimi, Makram Agha, Douaa Moussa De Boe, Veerle Lagneaux, Laurence Meuleman, Philip Vanhaecke, Tamara Najar, Mehdi Cells Article Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies. MDPI 2020-04-08 /pmc/articles/PMC7226778/ /pubmed/32276503 http://dx.doi.org/10.3390/cells9040914 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article De Kock, Joery Rodrigues, Robim Marcelino Branson, Steven Verhoye, Lieven Colemonts-Vroninks, Haaike Rombaut, Matthias Boeckmans, Joost Neuckermans, Jessie Lequeue, Sien Buyl, Karolien Merimi, Makram Agha, Douaa Moussa De Boe, Veerle Lagneaux, Laurence Meuleman, Philip Vanhaecke, Tamara Najar, Mehdi Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells |
title | Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells |
title_full | Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells |
title_fullStr | Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells |
title_full_unstemmed | Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells |
title_short | Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells |
title_sort | inflammation alters the secretome and immunomodulatory properties of human skin-derived precursor cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226778/ https://www.ncbi.nlm.nih.gov/pubmed/32276503 http://dx.doi.org/10.3390/cells9040914 |
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