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Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells

Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key can...

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Autores principales: De Kock, Joery, Rodrigues, Robim Marcelino, Branson, Steven, Verhoye, Lieven, Colemonts-Vroninks, Haaike, Rombaut, Matthias, Boeckmans, Joost, Neuckermans, Jessie, Lequeue, Sien, Buyl, Karolien, Merimi, Makram, Agha, Douaa Moussa, De Boe, Veerle, Lagneaux, Laurence, Meuleman, Philip, Vanhaecke, Tamara, Najar, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226778/
https://www.ncbi.nlm.nih.gov/pubmed/32276503
http://dx.doi.org/10.3390/cells9040914
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author De Kock, Joery
Rodrigues, Robim Marcelino
Branson, Steven
Verhoye, Lieven
Colemonts-Vroninks, Haaike
Rombaut, Matthias
Boeckmans, Joost
Neuckermans, Jessie
Lequeue, Sien
Buyl, Karolien
Merimi, Makram
Agha, Douaa Moussa
De Boe, Veerle
Lagneaux, Laurence
Meuleman, Philip
Vanhaecke, Tamara
Najar, Mehdi
author_facet De Kock, Joery
Rodrigues, Robim Marcelino
Branson, Steven
Verhoye, Lieven
Colemonts-Vroninks, Haaike
Rombaut, Matthias
Boeckmans, Joost
Neuckermans, Jessie
Lequeue, Sien
Buyl, Karolien
Merimi, Makram
Agha, Douaa Moussa
De Boe, Veerle
Lagneaux, Laurence
Meuleman, Philip
Vanhaecke, Tamara
Najar, Mehdi
author_sort De Kock, Joery
collection PubMed
description Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies.
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spelling pubmed-72267782020-05-18 Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells De Kock, Joery Rodrigues, Robim Marcelino Branson, Steven Verhoye, Lieven Colemonts-Vroninks, Haaike Rombaut, Matthias Boeckmans, Joost Neuckermans, Jessie Lequeue, Sien Buyl, Karolien Merimi, Makram Agha, Douaa Moussa De Boe, Veerle Lagneaux, Laurence Meuleman, Philip Vanhaecke, Tamara Najar, Mehdi Cells Article Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies. MDPI 2020-04-08 /pmc/articles/PMC7226778/ /pubmed/32276503 http://dx.doi.org/10.3390/cells9040914 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
De Kock, Joery
Rodrigues, Robim Marcelino
Branson, Steven
Verhoye, Lieven
Colemonts-Vroninks, Haaike
Rombaut, Matthias
Boeckmans, Joost
Neuckermans, Jessie
Lequeue, Sien
Buyl, Karolien
Merimi, Makram
Agha, Douaa Moussa
De Boe, Veerle
Lagneaux, Laurence
Meuleman, Philip
Vanhaecke, Tamara
Najar, Mehdi
Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells
title Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells
title_full Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells
title_fullStr Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells
title_full_unstemmed Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells
title_short Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells
title_sort inflammation alters the secretome and immunomodulatory properties of human skin-derived precursor cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226778/
https://www.ncbi.nlm.nih.gov/pubmed/32276503
http://dx.doi.org/10.3390/cells9040914
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