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A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing
The application of human-induced pluripotent stem cells (hiPSCs) to generate vascular smooth muscle cells (hiPSC-VSMCs) in abundance is a promising strategy for vascular regeneration. While hiPSC-VSMCs have already been utilized for tissue-engineered vascular grafts and disease modeling, there is a...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226960/ https://www.ncbi.nlm.nih.gov/pubmed/32295218 http://dx.doi.org/10.3390/cells9040966 |
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author | Dash, Biraja C. Setia, Ocean Gorecka, Jolanta Peyvandi, Hassan Duan, Kaiti Lopes, Lara Nie, James Berthiaume, Francois Dardik, Alan Hsia, Henry C. |
author_facet | Dash, Biraja C. Setia, Ocean Gorecka, Jolanta Peyvandi, Hassan Duan, Kaiti Lopes, Lara Nie, James Berthiaume, Francois Dardik, Alan Hsia, Henry C. |
author_sort | Dash, Biraja C. |
collection | PubMed |
description | The application of human-induced pluripotent stem cells (hiPSCs) to generate vascular smooth muscle cells (hiPSC-VSMCs) in abundance is a promising strategy for vascular regeneration. While hiPSC-VSMCs have already been utilized for tissue-engineered vascular grafts and disease modeling, there is a lack of investigations exploring their therapeutic secretory factors. The objective of this manuscript was to understand how the biophysical property of a collagen-based scaffold dictates changes in the secretory function of hiPSC-VSMCs while developing hiPSC-VSMC-based therapy for durable regenerative wound healing. We investigated the effect of collagen fibrillar density (CFD) on hiPSC-VSMC’s paracrine secretion and cytokines via the construction of varying density of collagen scaffolds. Our study demonstrated that CFD is a key scaffold property that modulates the secretory function of hiPSC-VSMCs. This study lays the foundation for developing collagen-based scaffold materials for the delivery of hiPSC-VSMCs to promote regenerative healing through guiding paracrine signaling pathways. |
format | Online Article Text |
id | pubmed-7226960 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72269602020-05-18 A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing Dash, Biraja C. Setia, Ocean Gorecka, Jolanta Peyvandi, Hassan Duan, Kaiti Lopes, Lara Nie, James Berthiaume, Francois Dardik, Alan Hsia, Henry C. Cells Article The application of human-induced pluripotent stem cells (hiPSCs) to generate vascular smooth muscle cells (hiPSC-VSMCs) in abundance is a promising strategy for vascular regeneration. While hiPSC-VSMCs have already been utilized for tissue-engineered vascular grafts and disease modeling, there is a lack of investigations exploring their therapeutic secretory factors. The objective of this manuscript was to understand how the biophysical property of a collagen-based scaffold dictates changes in the secretory function of hiPSC-VSMCs while developing hiPSC-VSMC-based therapy for durable regenerative wound healing. We investigated the effect of collagen fibrillar density (CFD) on hiPSC-VSMC’s paracrine secretion and cytokines via the construction of varying density of collagen scaffolds. Our study demonstrated that CFD is a key scaffold property that modulates the secretory function of hiPSC-VSMCs. This study lays the foundation for developing collagen-based scaffold materials for the delivery of hiPSC-VSMCs to promote regenerative healing through guiding paracrine signaling pathways. MDPI 2020-04-14 /pmc/articles/PMC7226960/ /pubmed/32295218 http://dx.doi.org/10.3390/cells9040966 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dash, Biraja C. Setia, Ocean Gorecka, Jolanta Peyvandi, Hassan Duan, Kaiti Lopes, Lara Nie, James Berthiaume, Francois Dardik, Alan Hsia, Henry C. A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing |
title | A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing |
title_full | A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing |
title_fullStr | A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing |
title_full_unstemmed | A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing |
title_short | A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing |
title_sort | dense fibrillar collagen scaffold differentially modulates secretory function of ipsc-derived vascular smooth muscle cells to promote wound healing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226960/ https://www.ncbi.nlm.nih.gov/pubmed/32295218 http://dx.doi.org/10.3390/cells9040966 |
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