Cargando…

Transplantation of Endothelial Progenitor Cells in Obese Diabetic Rats Following Myocardial Infarction: Role of Thymosin Beta-4

Endothelial progenitor cells (EPCs) are bone-marrow derived cells that are critical in the maintenance of endothelial wall integrity and protection of ischemic myocardium through the formation of new blood vessels (vasculogenesis) or proliferation of pre-existing vasculature (angiogenesis). Diabetes...

Descripción completa

Detalles Bibliográficos
Autores principales: Poh, Kian Keong, Lee, Poay Sian Sabrina, Djohan, Andie Hartanto, Galupo, Mary Joyce, Songco, Geronica Gorospe, Yeo, Tiong Cheng, Tan, Huay Cheem, Richards, Arthur Mark, Ye, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226991/
https://www.ncbi.nlm.nih.gov/pubmed/32290541
http://dx.doi.org/10.3390/cells9040949
_version_ 1783534406810468352
author Poh, Kian Keong
Lee, Poay Sian Sabrina
Djohan, Andie Hartanto
Galupo, Mary Joyce
Songco, Geronica Gorospe
Yeo, Tiong Cheng
Tan, Huay Cheem
Richards, Arthur Mark
Ye, Lei
author_facet Poh, Kian Keong
Lee, Poay Sian Sabrina
Djohan, Andie Hartanto
Galupo, Mary Joyce
Songco, Geronica Gorospe
Yeo, Tiong Cheng
Tan, Huay Cheem
Richards, Arthur Mark
Ye, Lei
author_sort Poh, Kian Keong
collection PubMed
description Endothelial progenitor cells (EPCs) are bone-marrow derived cells that are critical in the maintenance of endothelial wall integrity and protection of ischemic myocardium through the formation of new blood vessels (vasculogenesis) or proliferation of pre-existing vasculature (angiogenesis). Diabetes mellitus (DM) and the metabolic syndrome are commonly associated with ischemic heart disease through its pathological effects on the endothelium and consequent endothelial dysfunction. Thymosin-β4 (Tβ4) which expressed in the embryonic heart is critical in epicardial and coronary artery formation. In this study, we explored the effects of Tβ4 treatment on diabetic EPCs in vitro and intramyocardial injection of Tβ4-treated and non-Tβ4 treated EPCs following acute myocardial infarction (MI) of diabetic rats in vivo. It was found that 10 ng/mL Tβ4 increased migration, tubule formation, and angiogenic factor secretion of diabetic EPCs in vitro. In vivo, although implantation of Tβ4 treated diabetic EPCs significantly increased capillary density and attracted more c-Kit positive progenitor cells into the infarcted hearts as compared with implantation of non-Tβ4 treated diabetic EPCs, the significantly improved left ventricular ejection fraction was only found in the rats which received non-Tβ4 treated EPCs. The data suggests that a low dose Tβ4 increases diabetic EPC migration, tubule formation, and angiogenic factor secretion. However, it did not improve the effects of EPCs on left ventricular pump function in diabetic rats with MI.
format Online
Article
Text
id pubmed-7226991
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72269912020-05-18 Transplantation of Endothelial Progenitor Cells in Obese Diabetic Rats Following Myocardial Infarction: Role of Thymosin Beta-4 Poh, Kian Keong Lee, Poay Sian Sabrina Djohan, Andie Hartanto Galupo, Mary Joyce Songco, Geronica Gorospe Yeo, Tiong Cheng Tan, Huay Cheem Richards, Arthur Mark Ye, Lei Cells Article Endothelial progenitor cells (EPCs) are bone-marrow derived cells that are critical in the maintenance of endothelial wall integrity and protection of ischemic myocardium through the formation of new blood vessels (vasculogenesis) or proliferation of pre-existing vasculature (angiogenesis). Diabetes mellitus (DM) and the metabolic syndrome are commonly associated with ischemic heart disease through its pathological effects on the endothelium and consequent endothelial dysfunction. Thymosin-β4 (Tβ4) which expressed in the embryonic heart is critical in epicardial and coronary artery formation. In this study, we explored the effects of Tβ4 treatment on diabetic EPCs in vitro and intramyocardial injection of Tβ4-treated and non-Tβ4 treated EPCs following acute myocardial infarction (MI) of diabetic rats in vivo. It was found that 10 ng/mL Tβ4 increased migration, tubule formation, and angiogenic factor secretion of diabetic EPCs in vitro. In vivo, although implantation of Tβ4 treated diabetic EPCs significantly increased capillary density and attracted more c-Kit positive progenitor cells into the infarcted hearts as compared with implantation of non-Tβ4 treated diabetic EPCs, the significantly improved left ventricular ejection fraction was only found in the rats which received non-Tβ4 treated EPCs. The data suggests that a low dose Tβ4 increases diabetic EPC migration, tubule formation, and angiogenic factor secretion. However, it did not improve the effects of EPCs on left ventricular pump function in diabetic rats with MI. MDPI 2020-04-12 /pmc/articles/PMC7226991/ /pubmed/32290541 http://dx.doi.org/10.3390/cells9040949 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Poh, Kian Keong
Lee, Poay Sian Sabrina
Djohan, Andie Hartanto
Galupo, Mary Joyce
Songco, Geronica Gorospe
Yeo, Tiong Cheng
Tan, Huay Cheem
Richards, Arthur Mark
Ye, Lei
Transplantation of Endothelial Progenitor Cells in Obese Diabetic Rats Following Myocardial Infarction: Role of Thymosin Beta-4
title Transplantation of Endothelial Progenitor Cells in Obese Diabetic Rats Following Myocardial Infarction: Role of Thymosin Beta-4
title_full Transplantation of Endothelial Progenitor Cells in Obese Diabetic Rats Following Myocardial Infarction: Role of Thymosin Beta-4
title_fullStr Transplantation of Endothelial Progenitor Cells in Obese Diabetic Rats Following Myocardial Infarction: Role of Thymosin Beta-4
title_full_unstemmed Transplantation of Endothelial Progenitor Cells in Obese Diabetic Rats Following Myocardial Infarction: Role of Thymosin Beta-4
title_short Transplantation of Endothelial Progenitor Cells in Obese Diabetic Rats Following Myocardial Infarction: Role of Thymosin Beta-4
title_sort transplantation of endothelial progenitor cells in obese diabetic rats following myocardial infarction: role of thymosin beta-4
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226991/
https://www.ncbi.nlm.nih.gov/pubmed/32290541
http://dx.doi.org/10.3390/cells9040949
work_keys_str_mv AT pohkiankeong transplantationofendothelialprogenitorcellsinobesediabeticratsfollowingmyocardialinfarctionroleofthymosinbeta4
AT leepoaysiansabrina transplantationofendothelialprogenitorcellsinobesediabeticratsfollowingmyocardialinfarctionroleofthymosinbeta4
AT djohanandiehartanto transplantationofendothelialprogenitorcellsinobesediabeticratsfollowingmyocardialinfarctionroleofthymosinbeta4
AT galupomaryjoyce transplantationofendothelialprogenitorcellsinobesediabeticratsfollowingmyocardialinfarctionroleofthymosinbeta4
AT songcogeronicagorospe transplantationofendothelialprogenitorcellsinobesediabeticratsfollowingmyocardialinfarctionroleofthymosinbeta4
AT yeotiongcheng transplantationofendothelialprogenitorcellsinobesediabeticratsfollowingmyocardialinfarctionroleofthymosinbeta4
AT tanhuaycheem transplantationofendothelialprogenitorcellsinobesediabeticratsfollowingmyocardialinfarctionroleofthymosinbeta4
AT richardsarthurmark transplantationofendothelialprogenitorcellsinobesediabeticratsfollowingmyocardialinfarctionroleofthymosinbeta4
AT yelei transplantationofendothelialprogenitorcellsinobesediabeticratsfollowingmyocardialinfarctionroleofthymosinbeta4