Tracking Longitudinal Rotation of Silicon Nanowires for Biointerfaces

[Image: see text] The rolling motion (i.e., longitudinal rotation) of nanomaterials may serve as a proxy to probe microscopic environments. Furthermore, nanoscale rotations in biological systems are common but difficult to measure. Here, we report a new tool that measures rolling motion of a nanowire with a short arm grown at one end. We present a particle detection algorithm with subpixel resolution and image segmentation with principal component analysis that enables precise and automated determination of the nanowire orientation. We show that the nanowires’ rolling dynamics can be significantly affected by their surroundings and demonstrate the probes’ ability to reflect different nanobio interactions. A non-cell-interacting nanowire undergoes rapid subdiffusive rotation, while a cell-interacting nanowire exhibits superdiffusive unidirectional rotation when the cell membrane actively interacts with the nanowire and slow subdiffusive rotation when it is fully encompassed by the cell. Our method can be used to yield insights into various biophysical and assembly processes.