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Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG)

BACKGROUND: The mammalian intestinal tract harbors diverse and dynamic microbial communities that play pivotal roles in host health, metabolism, immunity, and development. Average daily gain (ADG) is an important growth trait in meat rabbit industry. The effects of gut microbiota on ADG in meat rabb...

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Autores principales: Fang, Shaoming, Chen, Xuan, Pan, Jiahua, Chen, Qiaohui, Zhou, Liwen, Wang, Chongchong, Xiao, Tianfang, Gan, Qian Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227296/
https://www.ncbi.nlm.nih.gov/pubmed/32410629
http://dx.doi.org/10.1186/s12866-020-01797-5
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author Fang, Shaoming
Chen, Xuan
Pan, Jiahua
Chen, Qiaohui
Zhou, Liwen
Wang, Chongchong
Xiao, Tianfang
Gan, Qian Fu
author_facet Fang, Shaoming
Chen, Xuan
Pan, Jiahua
Chen, Qiaohui
Zhou, Liwen
Wang, Chongchong
Xiao, Tianfang
Gan, Qian Fu
author_sort Fang, Shaoming
collection PubMed
description BACKGROUND: The mammalian intestinal tract harbors diverse and dynamic microbial communities that play pivotal roles in host health, metabolism, immunity, and development. Average daily gain (ADG) is an important growth trait in meat rabbit industry. The effects of gut microbiota on ADG in meat rabbits are still unknown. RESULTS: In this study, we investigated the dynamic distribution of gut microbiota in commercial Ira rabbits from weaning to finishing and uncover the relationship between the microbiota and average daily gain (ADG) via 16S rRNA gene sequencing. The results indicated that the richness and diversity of gut microbiota significantly increased with age. Gut microbial structure was less variable among finishing rabbits than among weaning rabbits. The relative abundances of the dominant phyla Firmicutes, Bacteroidetes, Verrucomicrobia and Cyanobacteria, and the 15 predominant genera significantly varied with age. Metagenomic prediction analysis showed that both KOs and KEGG pathways related to the metabolism of monosaccharides and vitamins were enriched in the weaning rabbits, while those related to the metabolism of amino acids and polysaccharides were more abundant in the finishing rabbits. We identified 34 OTUs, 125 KOs, and 25 KEGG pathways that were significantly associated with ADG. OTUs annotation suggested that butyrate producing bacteria belong to the family Ruminococcaceae and Bacteroidales_S24-7_group were positively associated with ADG. Conversely, Eubacterium_coprostanoligenes_group, Christensenellaceae_R-7_group, and opportunistic pathogens were negatively associated with ADG. Both KOs and KEGG pathways correlated with the metabolism of vitamins, basic amino acids, and short chain fatty acids (SCFAs) showed positive correlations with ADG, while those correlated with aromatic amino acids metabolism and immune response exhibited negative correlations with ADG. In addition, our results suggested that 10.42% of the variation in weaning weight could be explained by the gut microbiome. CONCLUSIONS: Our findings give a glimpse into the dynamic shifts in gut microbiota of meat rabbits and provide a theoretical basis for gut microbiota modulation to improve ADG in the meat rabbit industry.
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spelling pubmed-72272962020-05-27 Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG) Fang, Shaoming Chen, Xuan Pan, Jiahua Chen, Qiaohui Zhou, Liwen Wang, Chongchong Xiao, Tianfang Gan, Qian Fu BMC Microbiol Research Article BACKGROUND: The mammalian intestinal tract harbors diverse and dynamic microbial communities that play pivotal roles in host health, metabolism, immunity, and development. Average daily gain (ADG) is an important growth trait in meat rabbit industry. The effects of gut microbiota on ADG in meat rabbits are still unknown. RESULTS: In this study, we investigated the dynamic distribution of gut microbiota in commercial Ira rabbits from weaning to finishing and uncover the relationship between the microbiota and average daily gain (ADG) via 16S rRNA gene sequencing. The results indicated that the richness and diversity of gut microbiota significantly increased with age. Gut microbial structure was less variable among finishing rabbits than among weaning rabbits. The relative abundances of the dominant phyla Firmicutes, Bacteroidetes, Verrucomicrobia and Cyanobacteria, and the 15 predominant genera significantly varied with age. Metagenomic prediction analysis showed that both KOs and KEGG pathways related to the metabolism of monosaccharides and vitamins were enriched in the weaning rabbits, while those related to the metabolism of amino acids and polysaccharides were more abundant in the finishing rabbits. We identified 34 OTUs, 125 KOs, and 25 KEGG pathways that were significantly associated with ADG. OTUs annotation suggested that butyrate producing bacteria belong to the family Ruminococcaceae and Bacteroidales_S24-7_group were positively associated with ADG. Conversely, Eubacterium_coprostanoligenes_group, Christensenellaceae_R-7_group, and opportunistic pathogens were negatively associated with ADG. Both KOs and KEGG pathways correlated with the metabolism of vitamins, basic amino acids, and short chain fatty acids (SCFAs) showed positive correlations with ADG, while those correlated with aromatic amino acids metabolism and immune response exhibited negative correlations with ADG. In addition, our results suggested that 10.42% of the variation in weaning weight could be explained by the gut microbiome. CONCLUSIONS: Our findings give a glimpse into the dynamic shifts in gut microbiota of meat rabbits and provide a theoretical basis for gut microbiota modulation to improve ADG in the meat rabbit industry. BioMed Central 2020-05-14 /pmc/articles/PMC7227296/ /pubmed/32410629 http://dx.doi.org/10.1186/s12866-020-01797-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Fang, Shaoming
Chen, Xuan
Pan, Jiahua
Chen, Qiaohui
Zhou, Liwen
Wang, Chongchong
Xiao, Tianfang
Gan, Qian Fu
Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG)
title Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG)
title_full Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG)
title_fullStr Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG)
title_full_unstemmed Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG)
title_short Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG)
title_sort dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (adg)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227296/
https://www.ncbi.nlm.nih.gov/pubmed/32410629
http://dx.doi.org/10.1186/s12866-020-01797-5
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