Cargando…
Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG)
BACKGROUND: The mammalian intestinal tract harbors diverse and dynamic microbial communities that play pivotal roles in host health, metabolism, immunity, and development. Average daily gain (ADG) is an important growth trait in meat rabbit industry. The effects of gut microbiota on ADG in meat rabb...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227296/ https://www.ncbi.nlm.nih.gov/pubmed/32410629 http://dx.doi.org/10.1186/s12866-020-01797-5 |
_version_ | 1783534474549526528 |
---|---|
author | Fang, Shaoming Chen, Xuan Pan, Jiahua Chen, Qiaohui Zhou, Liwen Wang, Chongchong Xiao, Tianfang Gan, Qian Fu |
author_facet | Fang, Shaoming Chen, Xuan Pan, Jiahua Chen, Qiaohui Zhou, Liwen Wang, Chongchong Xiao, Tianfang Gan, Qian Fu |
author_sort | Fang, Shaoming |
collection | PubMed |
description | BACKGROUND: The mammalian intestinal tract harbors diverse and dynamic microbial communities that play pivotal roles in host health, metabolism, immunity, and development. Average daily gain (ADG) is an important growth trait in meat rabbit industry. The effects of gut microbiota on ADG in meat rabbits are still unknown. RESULTS: In this study, we investigated the dynamic distribution of gut microbiota in commercial Ira rabbits from weaning to finishing and uncover the relationship between the microbiota and average daily gain (ADG) via 16S rRNA gene sequencing. The results indicated that the richness and diversity of gut microbiota significantly increased with age. Gut microbial structure was less variable among finishing rabbits than among weaning rabbits. The relative abundances of the dominant phyla Firmicutes, Bacteroidetes, Verrucomicrobia and Cyanobacteria, and the 15 predominant genera significantly varied with age. Metagenomic prediction analysis showed that both KOs and KEGG pathways related to the metabolism of monosaccharides and vitamins were enriched in the weaning rabbits, while those related to the metabolism of amino acids and polysaccharides were more abundant in the finishing rabbits. We identified 34 OTUs, 125 KOs, and 25 KEGG pathways that were significantly associated with ADG. OTUs annotation suggested that butyrate producing bacteria belong to the family Ruminococcaceae and Bacteroidales_S24-7_group were positively associated with ADG. Conversely, Eubacterium_coprostanoligenes_group, Christensenellaceae_R-7_group, and opportunistic pathogens were negatively associated with ADG. Both KOs and KEGG pathways correlated with the metabolism of vitamins, basic amino acids, and short chain fatty acids (SCFAs) showed positive correlations with ADG, while those correlated with aromatic amino acids metabolism and immune response exhibited negative correlations with ADG. In addition, our results suggested that 10.42% of the variation in weaning weight could be explained by the gut microbiome. CONCLUSIONS: Our findings give a glimpse into the dynamic shifts in gut microbiota of meat rabbits and provide a theoretical basis for gut microbiota modulation to improve ADG in the meat rabbit industry. |
format | Online Article Text |
id | pubmed-7227296 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-72272962020-05-27 Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG) Fang, Shaoming Chen, Xuan Pan, Jiahua Chen, Qiaohui Zhou, Liwen Wang, Chongchong Xiao, Tianfang Gan, Qian Fu BMC Microbiol Research Article BACKGROUND: The mammalian intestinal tract harbors diverse and dynamic microbial communities that play pivotal roles in host health, metabolism, immunity, and development. Average daily gain (ADG) is an important growth trait in meat rabbit industry. The effects of gut microbiota on ADG in meat rabbits are still unknown. RESULTS: In this study, we investigated the dynamic distribution of gut microbiota in commercial Ira rabbits from weaning to finishing and uncover the relationship between the microbiota and average daily gain (ADG) via 16S rRNA gene sequencing. The results indicated that the richness and diversity of gut microbiota significantly increased with age. Gut microbial structure was less variable among finishing rabbits than among weaning rabbits. The relative abundances of the dominant phyla Firmicutes, Bacteroidetes, Verrucomicrobia and Cyanobacteria, and the 15 predominant genera significantly varied with age. Metagenomic prediction analysis showed that both KOs and KEGG pathways related to the metabolism of monosaccharides and vitamins were enriched in the weaning rabbits, while those related to the metabolism of amino acids and polysaccharides were more abundant in the finishing rabbits. We identified 34 OTUs, 125 KOs, and 25 KEGG pathways that were significantly associated with ADG. OTUs annotation suggested that butyrate producing bacteria belong to the family Ruminococcaceae and Bacteroidales_S24-7_group were positively associated with ADG. Conversely, Eubacterium_coprostanoligenes_group, Christensenellaceae_R-7_group, and opportunistic pathogens were negatively associated with ADG. Both KOs and KEGG pathways correlated with the metabolism of vitamins, basic amino acids, and short chain fatty acids (SCFAs) showed positive correlations with ADG, while those correlated with aromatic amino acids metabolism and immune response exhibited negative correlations with ADG. In addition, our results suggested that 10.42% of the variation in weaning weight could be explained by the gut microbiome. CONCLUSIONS: Our findings give a glimpse into the dynamic shifts in gut microbiota of meat rabbits and provide a theoretical basis for gut microbiota modulation to improve ADG in the meat rabbit industry. BioMed Central 2020-05-14 /pmc/articles/PMC7227296/ /pubmed/32410629 http://dx.doi.org/10.1186/s12866-020-01797-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Fang, Shaoming Chen, Xuan Pan, Jiahua Chen, Qiaohui Zhou, Liwen Wang, Chongchong Xiao, Tianfang Gan, Qian Fu Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG) |
title | Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG) |
title_full | Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG) |
title_fullStr | Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG) |
title_full_unstemmed | Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG) |
title_short | Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG) |
title_sort | dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (adg) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227296/ https://www.ncbi.nlm.nih.gov/pubmed/32410629 http://dx.doi.org/10.1186/s12866-020-01797-5 |
work_keys_str_mv | AT fangshaoming dynamicdistributionofgutmicrobiotainmeatrabbitsatdifferentgrowthstagesandrelationshipwithaveragedailygainadg AT chenxuan dynamicdistributionofgutmicrobiotainmeatrabbitsatdifferentgrowthstagesandrelationshipwithaveragedailygainadg AT panjiahua dynamicdistributionofgutmicrobiotainmeatrabbitsatdifferentgrowthstagesandrelationshipwithaveragedailygainadg AT chenqiaohui dynamicdistributionofgutmicrobiotainmeatrabbitsatdifferentgrowthstagesandrelationshipwithaveragedailygainadg AT zhouliwen dynamicdistributionofgutmicrobiotainmeatrabbitsatdifferentgrowthstagesandrelationshipwithaveragedailygainadg AT wangchongchong dynamicdistributionofgutmicrobiotainmeatrabbitsatdifferentgrowthstagesandrelationshipwithaveragedailygainadg AT xiaotianfang dynamicdistributionofgutmicrobiotainmeatrabbitsatdifferentgrowthstagesandrelationshipwithaveragedailygainadg AT ganqianfu dynamicdistributionofgutmicrobiotainmeatrabbitsatdifferentgrowthstagesandrelationshipwithaveragedailygainadg |