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Molecular identification and subtyping of Blastocystis sp. in laboratory rats in China
Blastocystis sp. is a ubiquitous protist that has been frequently reported in humans and animals worldwide. A total of 355 fecal samples of experimental rats were collected from four laboratory rearing facilities in China, and Blastocystis sp. was detected by PCR amplification of the partial small s...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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EDP Sciences
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227369/ https://www.ncbi.nlm.nih.gov/pubmed/32410727 http://dx.doi.org/10.1051/parasite/2020035 |
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author | Li, Junqiang Yuan, Yueyue Jiang, Yuxi Wang, Wen Chao, Liqin Sun, Ruiqin Li, Jun Karim, Md Robiul Qi, Meng |
author_facet | Li, Junqiang Yuan, Yueyue Jiang, Yuxi Wang, Wen Chao, Liqin Sun, Ruiqin Li, Jun Karim, Md Robiul Qi, Meng |
author_sort | Li, Junqiang |
collection | PubMed |
description | Blastocystis sp. is a ubiquitous protist that has been frequently reported in humans and animals worldwide. A total of 355 fecal samples of experimental rats were collected from four laboratory rearing facilities in China, and Blastocystis sp. was detected by PCR amplification of the partial small subunit ribosomal (SSU) rRNA gene. Twenty-nine (8.2%, 29/355) samples were positive for Blastocystis sp., with the highest infection rate (20.7%, 24/116) in rats of the Zhengzhou1, followed by that in the Zhengzhou2 (5.0%, 2/40), Shenyang (3.0%, 3/100) and Wuhan (0) rearing facilities. Among the three rat strains, Sprague–Dawley (SD) rats had higher infection rates (11.3%, 17/151) compared to Wistar rats (8.7%, 9/104) and spontaneously hypertensive (SH) rats (3.0%, 3/100). Two Blastocystis sp. subtypes (ST4 and ST7) were identified. ST4 was the predominant subtype detected in 26 samples (89.7%). A phylogenetic analysis demonstrated that the sequences of ST4 and ST7 obtained in this study were clustered with their reference subtypes. To our knowledge, this is the first report of Blastocystis sp. in experimental rats in China. Pathogen infections in laboratory animals need to be monitored due to fecal-oral transmission. |
format | Online Article Text |
id | pubmed-7227369 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-72273692020-05-20 Molecular identification and subtyping of Blastocystis sp. in laboratory rats in China Li, Junqiang Yuan, Yueyue Jiang, Yuxi Wang, Wen Chao, Liqin Sun, Ruiqin Li, Jun Karim, Md Robiul Qi, Meng Parasite Short Note Blastocystis sp. is a ubiquitous protist that has been frequently reported in humans and animals worldwide. A total of 355 fecal samples of experimental rats were collected from four laboratory rearing facilities in China, and Blastocystis sp. was detected by PCR amplification of the partial small subunit ribosomal (SSU) rRNA gene. Twenty-nine (8.2%, 29/355) samples were positive for Blastocystis sp., with the highest infection rate (20.7%, 24/116) in rats of the Zhengzhou1, followed by that in the Zhengzhou2 (5.0%, 2/40), Shenyang (3.0%, 3/100) and Wuhan (0) rearing facilities. Among the three rat strains, Sprague–Dawley (SD) rats had higher infection rates (11.3%, 17/151) compared to Wistar rats (8.7%, 9/104) and spontaneously hypertensive (SH) rats (3.0%, 3/100). Two Blastocystis sp. subtypes (ST4 and ST7) were identified. ST4 was the predominant subtype detected in 26 samples (89.7%). A phylogenetic analysis demonstrated that the sequences of ST4 and ST7 obtained in this study were clustered with their reference subtypes. To our knowledge, this is the first report of Blastocystis sp. in experimental rats in China. Pathogen infections in laboratory animals need to be monitored due to fecal-oral transmission. EDP Sciences 2020-05-15 /pmc/articles/PMC7227369/ /pubmed/32410727 http://dx.doi.org/10.1051/parasite/2020035 Text en © J. Li et al., published by EDP Sciences, 2020 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Note Li, Junqiang Yuan, Yueyue Jiang, Yuxi Wang, Wen Chao, Liqin Sun, Ruiqin Li, Jun Karim, Md Robiul Qi, Meng Molecular identification and subtyping of Blastocystis sp. in laboratory rats in China |
title | Molecular identification and subtyping of Blastocystis sp. in laboratory rats in China |
title_full | Molecular identification and subtyping of Blastocystis sp. in laboratory rats in China |
title_fullStr | Molecular identification and subtyping of Blastocystis sp. in laboratory rats in China |
title_full_unstemmed | Molecular identification and subtyping of Blastocystis sp. in laboratory rats in China |
title_short | Molecular identification and subtyping of Blastocystis sp. in laboratory rats in China |
title_sort | molecular identification and subtyping of blastocystis sp. in laboratory rats in china |
topic | Short Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227369/ https://www.ncbi.nlm.nih.gov/pubmed/32410727 http://dx.doi.org/10.1051/parasite/2020035 |
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