Genetic Mapping of a Light-Dependent Lesion Mimic Mutant Reveals the Function of Coproporphyrinogen III Oxidase Homolog in Soybean

Lesion mimic mutants provide ideal genetic materials for elucidating the molecular mechanism of cell death and disease resistance. Here, we isolated a Glycine max lesion mimic mutant 2-1 (Gmlmm2-1), which displayed a light-dependent cell death phenotype. Map-based cloning revealed that GmLMM2 encods...

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Autores principales: Ma, Jingjing, Yang, Suxin, Wang, Dongmei, Tang, Kuanqiang, Feng, Xing Xing, Feng, Xian Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227399/
https://www.ncbi.nlm.nih.gov/pubmed/32457787
http://dx.doi.org/10.3389/fpls.2020.00557
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author Ma, Jingjing
Yang, Suxin
Wang, Dongmei
Tang, Kuanqiang
Feng, Xing Xing
Feng, Xian Zhong
author_facet Ma, Jingjing
Yang, Suxin
Wang, Dongmei
Tang, Kuanqiang
Feng, Xing Xing
Feng, Xian Zhong
author_sort Ma, Jingjing
collection PubMed
description Lesion mimic mutants provide ideal genetic materials for elucidating the molecular mechanism of cell death and disease resistance. Here, we isolated a Glycine max lesion mimic mutant 2-1 (Gmlmm2-1), which displayed a light-dependent cell death phenotype. Map-based cloning revealed that GmLMM2 encods a coproporphyrinogen III oxidase and participates in tetrapyrrole biosynthesis. Knockout of GmLMM2 led to necrotic spots on developing leaves of CRISPR/Cas9 induced mutants. The GmLMM2 defect decreased the chlorophyll content by disrupting tetrapyrrole biosynthesis and enhanced resistance to Phytophthora sojae. These results suggested that GmLMM2 gene played an important role in the biosynthesis of tetrapyrrole and light-dependent defense in soybeans.
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spelling pubmed-72273992020-05-25 Genetic Mapping of a Light-Dependent Lesion Mimic Mutant Reveals the Function of Coproporphyrinogen III Oxidase Homolog in Soybean Ma, Jingjing Yang, Suxin Wang, Dongmei Tang, Kuanqiang Feng, Xing Xing Feng, Xian Zhong Front Plant Sci Plant Science Lesion mimic mutants provide ideal genetic materials for elucidating the molecular mechanism of cell death and disease resistance. Here, we isolated a Glycine max lesion mimic mutant 2-1 (Gmlmm2-1), which displayed a light-dependent cell death phenotype. Map-based cloning revealed that GmLMM2 encods a coproporphyrinogen III oxidase and participates in tetrapyrrole biosynthesis. Knockout of GmLMM2 led to necrotic spots on developing leaves of CRISPR/Cas9 induced mutants. The GmLMM2 defect decreased the chlorophyll content by disrupting tetrapyrrole biosynthesis and enhanced resistance to Phytophthora sojae. These results suggested that GmLMM2 gene played an important role in the biosynthesis of tetrapyrrole and light-dependent defense in soybeans. Frontiers Media S.A. 2020-05-08 /pmc/articles/PMC7227399/ /pubmed/32457787 http://dx.doi.org/10.3389/fpls.2020.00557 Text en Copyright © 2020 Ma, Yang, Wang, Tang, Feng and Feng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ma, Jingjing
Yang, Suxin
Wang, Dongmei
Tang, Kuanqiang
Feng, Xing Xing
Feng, Xian Zhong
Genetic Mapping of a Light-Dependent Lesion Mimic Mutant Reveals the Function of Coproporphyrinogen III Oxidase Homolog in Soybean
title Genetic Mapping of a Light-Dependent Lesion Mimic Mutant Reveals the Function of Coproporphyrinogen III Oxidase Homolog in Soybean
title_full Genetic Mapping of a Light-Dependent Lesion Mimic Mutant Reveals the Function of Coproporphyrinogen III Oxidase Homolog in Soybean
title_fullStr Genetic Mapping of a Light-Dependent Lesion Mimic Mutant Reveals the Function of Coproporphyrinogen III Oxidase Homolog in Soybean
title_full_unstemmed Genetic Mapping of a Light-Dependent Lesion Mimic Mutant Reveals the Function of Coproporphyrinogen III Oxidase Homolog in Soybean
title_short Genetic Mapping of a Light-Dependent Lesion Mimic Mutant Reveals the Function of Coproporphyrinogen III Oxidase Homolog in Soybean
title_sort genetic mapping of a light-dependent lesion mimic mutant reveals the function of coproporphyrinogen iii oxidase homolog in soybean
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227399/
https://www.ncbi.nlm.nih.gov/pubmed/32457787
http://dx.doi.org/10.3389/fpls.2020.00557
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