Efficient targeted integration directed by short homology in zebrafish and mammalian cells

Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Here, we describe a set of resources to streamline reporter gene knock-ins in zebrafish and demonstrate the broader utility of the method in mammalian cells. Our approach uses sh...

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Detalles Bibliográficos
Autores principales: Wierson, Wesley A, Welker, Jordan M, Almeida, Maira P, Mann, Carla M, Webster, Dennis A, Torrie, Melanie E, Weiss, Trevor J, Kambakam, Sekhar, Vollbrecht, Macy K, Lan, Merrina, McKeighan, Kenna C, Levey, Jacklyn, Ming, Zhitao, Wehmeier, Alec, Mikelson, Christopher S, Haltom, Jeffrey A, Kwan, Kristen M, Chien, Chi-Bin, Balciunas, Darius, Ekker, Stephen C, Clark, Karl J, Webber, Beau R, Moriarity, Branden S, Solin, Stacy L, Carlson, Daniel F, Dobbs, Drena L, McGrail, Maura, Essner, Jeffrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228771/
https://www.ncbi.nlm.nih.gov/pubmed/32412410
http://dx.doi.org/10.7554/eLife.53968

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