Fluorescence strategies for mapping cell membrane dynamics and structures

Fluorescence spectroscopy has been a cornerstone of research in membrane dynamics and organization. Technological advances in fluorescence spectroscopy went hand in hand with discovery of various physicochemical properties of membranes at nanometric spatial and microsecond timescales. In this perspe...

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Detalles Bibliográficos
Autores principales: Sankaran, Jagadish, Wohland, Thorsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2020
Materias:
Perspectives
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228782/
https://www.ncbi.nlm.nih.gov/pubmed/32478279
http://dx.doi.org/10.1063/1.5143945
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author Sankaran, Jagadish
Wohland, Thorsten
author_facet Sankaran, Jagadish
Wohland, Thorsten
author_sort Sankaran, Jagadish
collection PubMed
description Fluorescence spectroscopy has been a cornerstone of research in membrane dynamics and organization. Technological advances in fluorescence spectroscopy went hand in hand with discovery of various physicochemical properties of membranes at nanometric spatial and microsecond timescales. In this perspective, we discuss the various challenges associated with quantification of physicochemical properties of membranes and how various modes of fluorescence spectroscopy have overcome these challenges to shed light on the structure and organization of membranes. Finally, we discuss newer measurement strategies and data analysis tools to investigate the structure, dynamics, and organization of membranes.
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spelling pubmed-72287822020-05-29 Fluorescence strategies for mapping cell membrane dynamics and structures Sankaran, Jagadish Wohland, Thorsten APL Bioeng Perspectives Fluorescence spectroscopy has been a cornerstone of research in membrane dynamics and organization. Technological advances in fluorescence spectroscopy went hand in hand with discovery of various physicochemical properties of membranes at nanometric spatial and microsecond timescales. In this perspective, we discuss the various challenges associated with quantification of physicochemical properties of membranes and how various modes of fluorescence spectroscopy have overcome these challenges to shed light on the structure and organization of membranes. Finally, we discuss newer measurement strategies and data analysis tools to investigate the structure, dynamics, and organization of membranes. AIP Publishing LLC 2020-05-12 /pmc/articles/PMC7228782/ /pubmed/32478279 http://dx.doi.org/10.1063/1.5143945 Text en © Author(s). 2473-2877/2020/4(2)/020901/15 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Perspectives
Sankaran, Jagadish
Wohland, Thorsten
Fluorescence strategies for mapping cell membrane dynamics and structures
title Fluorescence strategies for mapping cell membrane dynamics and structures
title_full Fluorescence strategies for mapping cell membrane dynamics and structures
title_fullStr Fluorescence strategies for mapping cell membrane dynamics and structures
title_full_unstemmed Fluorescence strategies for mapping cell membrane dynamics and structures
title_short Fluorescence strategies for mapping cell membrane dynamics and structures
title_sort fluorescence strategies for mapping cell membrane dynamics and structures
topic Perspectives
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228782/
https://www.ncbi.nlm.nih.gov/pubmed/32478279
http://dx.doi.org/10.1063/1.5143945
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AT wohlandthorsten fluorescencestrategiesformappingcellmembranedynamicsandstructures

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