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Alterations of Serum Uric Acid Level and Gut Microbiota After Roux-en-Y Gastric Bypass and Sleeve Gastrectomy in a Hyperuricemic Rat Model

BACKGROUND: The objective of this study was to observe alterations of serum uric acid (SUA) level and gut microbiota after Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG) surgery in a hyperuricemic rat model. METHOD: We performed Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG...

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Autores principales: Lu, Cunlong, Li, Yu, Li, Long, Kong, Ying, Shi, Tuo, Xiao, Hai, Cao, Shougen, Zhu, Houxin, Li, Zequn, Zhou, Yanbing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228899/
https://www.ncbi.nlm.nih.gov/pubmed/32124218
http://dx.doi.org/10.1007/s11695-019-04328-y
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author Lu, Cunlong
Li, Yu
Li, Long
Kong, Ying
Shi, Tuo
Xiao, Hai
Cao, Shougen
Zhu, Houxin
Li, Zequn
Zhou, Yanbing
author_facet Lu, Cunlong
Li, Yu
Li, Long
Kong, Ying
Shi, Tuo
Xiao, Hai
Cao, Shougen
Zhu, Houxin
Li, Zequn
Zhou, Yanbing
author_sort Lu, Cunlong
collection PubMed
description BACKGROUND: The objective of this study was to observe alterations of serum uric acid (SUA) level and gut microbiota after Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG) surgery in a hyperuricemic rat model. METHOD: We performed Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG) surgery in a hyperuricemic rat model. Serum uric acid (UA), xanthine oxidase (XO) activity, IL-6, TNF-α and lipopolysaccharide (LPS) level changes, and 16S rDNA of gut microbiota were analyzed. RESULTS: After the surgery, the RYGB and SG procedures significantly reduced body weight, serum UA, IL-6, TNF-α and LPS levels, and XO activity. In addition, the RYGB and SG procedures altered the diversity and taxonomic composition of the gut microbiota. Compared with Sham group, RYGB and SG procedures were enriched in the abundance of phylum Verrucomicrobia and species Akkermansia muciniphila, while the species Escherichia coli was reduced. DISCUSSION: We here concluded that bariatric surgery-induced weight loss and resolution of inflammatory remarkers as well as changes of gut microbiota may be responsible for the reduced XO activity and SUA level. To have a better understanding of the underlying mechanism of UA metabolism following bariatric surgery, further research is needed.
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spelling pubmed-72288992020-05-18 Alterations of Serum Uric Acid Level and Gut Microbiota After Roux-en-Y Gastric Bypass and Sleeve Gastrectomy in a Hyperuricemic Rat Model Lu, Cunlong Li, Yu Li, Long Kong, Ying Shi, Tuo Xiao, Hai Cao, Shougen Zhu, Houxin Li, Zequn Zhou, Yanbing Obes Surg Original Contributions BACKGROUND: The objective of this study was to observe alterations of serum uric acid (SUA) level and gut microbiota after Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG) surgery in a hyperuricemic rat model. METHOD: We performed Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG) surgery in a hyperuricemic rat model. Serum uric acid (UA), xanthine oxidase (XO) activity, IL-6, TNF-α and lipopolysaccharide (LPS) level changes, and 16S rDNA of gut microbiota were analyzed. RESULTS: After the surgery, the RYGB and SG procedures significantly reduced body weight, serum UA, IL-6, TNF-α and LPS levels, and XO activity. In addition, the RYGB and SG procedures altered the diversity and taxonomic composition of the gut microbiota. Compared with Sham group, RYGB and SG procedures were enriched in the abundance of phylum Verrucomicrobia and species Akkermansia muciniphila, while the species Escherichia coli was reduced. DISCUSSION: We here concluded that bariatric surgery-induced weight loss and resolution of inflammatory remarkers as well as changes of gut microbiota may be responsible for the reduced XO activity and SUA level. To have a better understanding of the underlying mechanism of UA metabolism following bariatric surgery, further research is needed. Springer US 2020-03-02 2020 /pmc/articles/PMC7228899/ /pubmed/32124218 http://dx.doi.org/10.1007/s11695-019-04328-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Contributions
Lu, Cunlong
Li, Yu
Li, Long
Kong, Ying
Shi, Tuo
Xiao, Hai
Cao, Shougen
Zhu, Houxin
Li, Zequn
Zhou, Yanbing
Alterations of Serum Uric Acid Level and Gut Microbiota After Roux-en-Y Gastric Bypass and Sleeve Gastrectomy in a Hyperuricemic Rat Model
title Alterations of Serum Uric Acid Level and Gut Microbiota After Roux-en-Y Gastric Bypass and Sleeve Gastrectomy in a Hyperuricemic Rat Model
title_full Alterations of Serum Uric Acid Level and Gut Microbiota After Roux-en-Y Gastric Bypass and Sleeve Gastrectomy in a Hyperuricemic Rat Model
title_fullStr Alterations of Serum Uric Acid Level and Gut Microbiota After Roux-en-Y Gastric Bypass and Sleeve Gastrectomy in a Hyperuricemic Rat Model
title_full_unstemmed Alterations of Serum Uric Acid Level and Gut Microbiota After Roux-en-Y Gastric Bypass and Sleeve Gastrectomy in a Hyperuricemic Rat Model
title_short Alterations of Serum Uric Acid Level and Gut Microbiota After Roux-en-Y Gastric Bypass and Sleeve Gastrectomy in a Hyperuricemic Rat Model
title_sort alterations of serum uric acid level and gut microbiota after roux-en-y gastric bypass and sleeve gastrectomy in a hyperuricemic rat model
topic Original Contributions
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228899/
https://www.ncbi.nlm.nih.gov/pubmed/32124218
http://dx.doi.org/10.1007/s11695-019-04328-y
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