Cargando…

Alteration of Protein Binding Affinities by Aqueous Two-Phase Systems Revealed by Pressure Perturbation

Interactions between proteins and ligands, which are fundamental to many biochemical processes essential to life, are mostly studied at dilute buffer conditions. The effects of the highly crowded nature of biological cells and the effects of liquid-liquid phase separation inducing biomolecular dropl...

Descripción completa

Detalles Bibliográficos
Autores principales:	Oliva, Rosario, Banerjee, Sudeshna, Cinar, Hasan, Ehrt, Christiane, Winter, Roland
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228918/ https://www.ncbi.nlm.nih.gov/pubmed/32415277 http://dx.doi.org/10.1038/s41598-020-65053-6

Ejemplares similares

The effects of cosolutes and crowding on the kinetics of protein condensate formation based on liquid–liquid phase separation: a pressure-jump relaxation study
por: Cinar, Hasan, et al.
Publicado: (2020)

Unraveling the binding characteristics of small ligands to telomeric DNA by pressure modulation
por: Oliva, Rosario, et al.
Publicado: (2021)

Pressure Sensitivity of SynGAP/PSD‐95 Condensates as a Model for Postsynaptic Densities and Its Biophysical and Neurological Ramifications
por: Cinar, Hasan, et al.
Publicado: (2020)

Suppression of Liquid‐Liquid Phase Separation and Aggregation of Antibodies by Modest Pressure Application
por: Fetahaj, Zamira, et al.
Publicado: (2022)

The measurement of binding affinities by NMR chemical shift perturbation
por: Hobbs, Billy, et al.
Publicado: (2022)

The Effects of Temperature and Pressure on Protein-Ligand Binding in the Presence of Mars-Relevant Salts †
por: Jahmidi-Azizi, Nisrine, et al.
Publicado: (2021)

Ions in the Deep Subsurface of Earth, Mars, and Icy Moons: Their Effects in Combination with Temperature and Pressure on tRNA–Ligand Binding
por: Jahmidi-Azizi, Nisrine, et al.
Publicado: (2021)

Predicting Binding Affinities for GPCR Ligands Using Free-Energy Perturbation
por: Lenselink, Eelke B., et al.
Publicado: (2016)

Alteration of the Conformational Dynamics of a DNA Hairpin by α‐Synuclein in the Presence of Aqueous Two‐Phase Systems
por: Mukherjee, Sanjib K., et al.
Publicado: (2020)

Macrocyclic squaramides: anion receptors with high sulfate binding affinity and selectivity in aqueous media
por: Qin, Lei, et al.
Publicado: (2016)

The N-terminal domain of the prion protein is required and sufficient for liquid–liquid phase separation: A crucial role of the Aβ-binding domain
por: Kamps, Janine, et al.
Publicado: (2021)

Change in Allosteric Network Affects Binding Affinities of PDZ Domains: Analysis through Perturbation Response Scanning
por: Gerek, Z. Nevin, et al.
Publicado: (2011)

A benchmark driven guide to binding site comparison: An exhaustive evaluation using tailor-made data sets (ProSPECCTs)
por: Ehrt, Christiane, et al.
Publicado: (2018)

Mutations in maltose-binding protein that alter affinity and solubility properties
por: Walker, Iris H., et al.
Publicado: (2010)

Perturbation of Cytochrome c Maturation Reveals Adaptability of the Respiratory Chain in Mycobacterium tuberculosis
por: Small, Jennifer L., et al.
Publicado: (2013)

Free Energy Perturbation Calculations of Mutation Effects on SARS-CoV-2 RBD::ACE2 Binding Affinity
por: Sergeeva, Alina P., et al.
Publicado: (2023)

Surfactant-free Colloidal Particles with Specific Binding Affinity
por: van der Wel, Casper, et al.
Publicado: (2017)

Photoionization of the aqueous phase: clusters, droplets and liquid jets
por: Signorell, Ruth, et al.
Publicado: (2022)

Binding Affinity and Driving Forces for the Interaction of Calixarene-Based Micellar Aggregates With Model Antibiotics in Neutral Aqueous Solution
por: Migliore, Rossella, et al.
Publicado: (2021)

Binding affinities of the farnesoid X receptor in the D3R Grand Challenge 2 estimated by free-energy perturbation and docking
por: Olsson, Martin A., et al.
Publicado: (2017)

Influence of length and flexibility of spacers on the binding affinity of divalent ligands
por: Liese, Susanne, et al.
Publicado: (2015)

Binding Properties of RNA Quadruplex of SARS-CoV-2 to Berberine Compared to Telomeric DNA Quadruplex
por: Oliva, Rosario, et al.
Publicado: (2022)

Structural basis for the dissociation of α-synuclein fibrils triggered by pressure perturbation of the hydrophobic core
por: de Oliveira, Guilherme A. P., et al.
Publicado: (2016)

FoldAffinity: binding affinities from nDSF experiments
por: Niebling, Stephan, et al.
Publicado: (2021)

Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase Systems
por: da Silva, Nuno R., et al.
Publicado: (2021)

Density-dependent cooperative non-specific binding in solid-phase SELEX affinity selection
por: Ozer, Abdullah, et al.
Publicado: (2013)

High-affinity RNA binding by a hyperthermophilic single-stranded DNA-binding protein
por: Morten, Michael J., et al.
Publicado: (2017)

Pharmacological perturbation of the phase-separating protein SMNDC1
por: Enders, Lennart, et al.
Publicado: (2023)

Inverse tuning of metal binding affinity and protein stability by altering charged coordination residues in designed calcium binding proteins
por: Maniccia, Anna Wilkins, et al.
Publicado: (2009)

Methods for Improving Aptamer Binding Affinity
por: Hasegawa, Hijiri, et al.
Publicado: (2016)

How to measure and evaluate binding affinities
por: Jarmoskaite, Inga, et al.
Publicado: (2020)

Intracellular drug binding affinities by NMR
por: Madl, Tobias
Publicado: (2021)

Calcium Binds to Transthyretin with Low Affinity
por: Cantarutti, Cristina, et al.
Publicado: (2022)

On the Rapid Calculation of Binding Affinities for Antigen and Antibody Design and Affinity Maturation Simulations
por: Conti, Simone, et al.
Publicado: (2022)

Low-affinity CTCF binding drives transcriptional regulation whereas high-affinity binding encompasses architectural functions
por: Marina-Zárate, Ester, et al.
Publicado: (2023)

Problems in perturbation
por: Nayfeh, Ali Hasan
Publicado: (1985)

Perturbation methods
por: Nayfeh, Ali Hasan
Publicado: (1973)

The effect of anisotropic stress and non-adiabatic pressure perturbations on the evolution of the comoving curvature perturbation
por: Naruko, Atsushi, et al.
Publicado: (2018)

Perturbation methods
por: Nayfeh, Ali Hasan, 1933-
Publicado: (2004)

Perturbation methods.
por: Nayfeh, Ali Hasan, 1933-
Publicado: (2000)

Cannot write session to /tmp/vufind_sessions/sess_dub66oa439to366f0vipontc5a