CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5

Gene repression using the endonucleolytically deactivated dCas9 protein and sgRNAs (CRISPR interference or CRISPRi) is a useful approach to study gene functions. Here, we established CRISPRi in Paenibacillus sonchi genomovar Riograndensis SBR5, a plant growth promoting bacterium. CRISPRi system with...

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Detalles Bibliográficos
Autores principales: Brito, Luciana F., Schultenkämper, Kerstin, Passaglia, Luciane M. P., Wendisch, Volker F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Methods and Protocols
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229006/
https://www.ncbi.nlm.nih.gov/pubmed/32274563
http://dx.doi.org/10.1007/s00253-020-10571-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229006/
https://www.ncbi.nlm.nih.gov/pubmed/32274563
http://dx.doi.org/10.1007/s00253-020-10571-6

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