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sMETASeq: Combined Profiling of Microbiota and Host Small RNAs
Understanding microbial communities' roles in human health and disease requires methods that accurately characterize the microbial composition and their activity and effects within human biological samples. We present sMETASeq (small RNA Metagenomics by Sequencing), a novel method that uses seq...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229328/ https://www.ncbi.nlm.nih.gov/pubmed/32422595 http://dx.doi.org/10.1016/j.isci.2020.101131 |
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author | Mjelle, Robin Aass, Kristin Roseth Sjursen, Wenche Hofsli, Eva Sætrom, Pål |
author_facet | Mjelle, Robin Aass, Kristin Roseth Sjursen, Wenche Hofsli, Eva Sætrom, Pål |
author_sort | Mjelle, Robin |
collection | PubMed |
description | Understanding microbial communities' roles in human health and disease requires methods that accurately characterize the microbial composition and their activity and effects within human biological samples. We present sMETASeq (small RNA Metagenomics by Sequencing), a novel method that uses sequencing of small RNAs to jointly measure host small RNA expression and create metagenomic profiles and detect small bacterial RNAs. We evaluated the performance of sMETASeq on a mock bacterial community and demonstrated its use on different human samples, including colon cancer, oral leukoplakia, cervix cancer, and a panel of human biofluids. In all datasets, the detected microbes reflected the biology of the different sample types. |
format | Online Article Text |
id | pubmed-7229328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-72293282020-05-20 sMETASeq: Combined Profiling of Microbiota and Host Small RNAs Mjelle, Robin Aass, Kristin Roseth Sjursen, Wenche Hofsli, Eva Sætrom, Pål iScience Article Understanding microbial communities' roles in human health and disease requires methods that accurately characterize the microbial composition and their activity and effects within human biological samples. We present sMETASeq (small RNA Metagenomics by Sequencing), a novel method that uses sequencing of small RNAs to jointly measure host small RNA expression and create metagenomic profiles and detect small bacterial RNAs. We evaluated the performance of sMETASeq on a mock bacterial community and demonstrated its use on different human samples, including colon cancer, oral leukoplakia, cervix cancer, and a panel of human biofluids. In all datasets, the detected microbes reflected the biology of the different sample types. Elsevier 2020-05-04 /pmc/articles/PMC7229328/ /pubmed/32422595 http://dx.doi.org/10.1016/j.isci.2020.101131 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Mjelle, Robin Aass, Kristin Roseth Sjursen, Wenche Hofsli, Eva Sætrom, Pål sMETASeq: Combined Profiling of Microbiota and Host Small RNAs |
title | sMETASeq: Combined Profiling of Microbiota and Host Small RNAs |
title_full | sMETASeq: Combined Profiling of Microbiota and Host Small RNAs |
title_fullStr | sMETASeq: Combined Profiling of Microbiota and Host Small RNAs |
title_full_unstemmed | sMETASeq: Combined Profiling of Microbiota and Host Small RNAs |
title_short | sMETASeq: Combined Profiling of Microbiota and Host Small RNAs |
title_sort | smetaseq: combined profiling of microbiota and host small rnas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229328/ https://www.ncbi.nlm.nih.gov/pubmed/32422595 http://dx.doi.org/10.1016/j.isci.2020.101131 |
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