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Evaluation of the proliferative potential of skin keratinocytes and fibroblasts isolated from critical limb ischemia patients

Impaired wound healing in critical limb ischemia (CLI) results from multiple factors that affect many cell types and their behavior. Epidermal keratinocytes and dermal fibroblasts play crucial roles in wound healing. However, it remains unclear whether these cell types irreversibly convert into a no...

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Autores principales: Toki, Fujio, Nanba, Daisuke, Nishimura, Emi K., Matsuzaki, Kyoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society for Regenerative Medicine 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229408/
https://www.ncbi.nlm.nih.gov/pubmed/32435674
http://dx.doi.org/10.1016/j.reth.2020.03.016
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author Toki, Fujio
Nanba, Daisuke
Nishimura, Emi K.
Matsuzaki, Kyoichi
author_facet Toki, Fujio
Nanba, Daisuke
Nishimura, Emi K.
Matsuzaki, Kyoichi
author_sort Toki, Fujio
collection PubMed
description Impaired wound healing in critical limb ischemia (CLI) results from multiple factors that affect many cell types and their behavior. Epidermal keratinocytes and dermal fibroblasts play crucial roles in wound healing. However, it remains unclear whether these cell types irreversibly convert into a non-proliferative phenotype and are involved in impaired wound healing in CLI. Here, we demonstrate that skin keratinocytes and fibroblasts isolated from CLI patients maintain their proliferative potentials. Epidermal keratinocytes and dermal fibroblasts were isolated from the surrounding skin of foot wounds in CLI patients with diabetic nephropathy on hemodialysis, and their growth potentials were evaluated. It was found that keratinocytes from lower limbs and trunk of patients can give rise to proliferative growing colonies and can be serially passaged. Fibroblasts can also form colonies with a proliferative phenotype. These results indicate that skin keratinocytes and fibroblasts maintain their proliferative capacity even in diabetic and ischemic microenvironments and can be reactivated under appropriate conditions. This study provides strong evidence that the improvement of the cellular microenvironments is a promising therapeutic approach for CLI and these cells can also be used for potential sources of skin reconstruction.
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spelling pubmed-72294082020-05-20 Evaluation of the proliferative potential of skin keratinocytes and fibroblasts isolated from critical limb ischemia patients Toki, Fujio Nanba, Daisuke Nishimura, Emi K. Matsuzaki, Kyoichi Regen Ther Original Article Impaired wound healing in critical limb ischemia (CLI) results from multiple factors that affect many cell types and their behavior. Epidermal keratinocytes and dermal fibroblasts play crucial roles in wound healing. However, it remains unclear whether these cell types irreversibly convert into a non-proliferative phenotype and are involved in impaired wound healing in CLI. Here, we demonstrate that skin keratinocytes and fibroblasts isolated from CLI patients maintain their proliferative potentials. Epidermal keratinocytes and dermal fibroblasts were isolated from the surrounding skin of foot wounds in CLI patients with diabetic nephropathy on hemodialysis, and their growth potentials were evaluated. It was found that keratinocytes from lower limbs and trunk of patients can give rise to proliferative growing colonies and can be serially passaged. Fibroblasts can also form colonies with a proliferative phenotype. These results indicate that skin keratinocytes and fibroblasts maintain their proliferative capacity even in diabetic and ischemic microenvironments and can be reactivated under appropriate conditions. This study provides strong evidence that the improvement of the cellular microenvironments is a promising therapeutic approach for CLI and these cells can also be used for potential sources of skin reconstruction. Japanese Society for Regenerative Medicine 2020-05-15 /pmc/articles/PMC7229408/ /pubmed/32435674 http://dx.doi.org/10.1016/j.reth.2020.03.016 Text en © 2020 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Toki, Fujio
Nanba, Daisuke
Nishimura, Emi K.
Matsuzaki, Kyoichi
Evaluation of the proliferative potential of skin keratinocytes and fibroblasts isolated from critical limb ischemia patients
title Evaluation of the proliferative potential of skin keratinocytes and fibroblasts isolated from critical limb ischemia patients
title_full Evaluation of the proliferative potential of skin keratinocytes and fibroblasts isolated from critical limb ischemia patients
title_fullStr Evaluation of the proliferative potential of skin keratinocytes and fibroblasts isolated from critical limb ischemia patients
title_full_unstemmed Evaluation of the proliferative potential of skin keratinocytes and fibroblasts isolated from critical limb ischemia patients
title_short Evaluation of the proliferative potential of skin keratinocytes and fibroblasts isolated from critical limb ischemia patients
title_sort evaluation of the proliferative potential of skin keratinocytes and fibroblasts isolated from critical limb ischemia patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229408/
https://www.ncbi.nlm.nih.gov/pubmed/32435674
http://dx.doi.org/10.1016/j.reth.2020.03.016
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