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Polyaniline Based Electrochemical Sensor for the Detection of Dengue Virus Infection

BACKGROUND: Dengue burden is increasing day-by-day globally. A rapid, sensitive, cost-effective early diagnosis kit is the need of the hour. In this study, a label-free electrochemical immunosensor was proposed for dengue virus detection. A modified Polyaniline (PANI) coated Glassy Carbon (GC) elect...

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Autores principales: Dutta, Reshmi, Thangapandi, K, Mondal, Sumantra, Nanda, Amalesh, Bose, Shreyosi, Sanyal, Shairee, Jana, Saikat Kumar, Ghorai, Suvankar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229450/
https://www.ncbi.nlm.nih.gov/pubmed/32431791
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author Dutta, Reshmi
Thangapandi, K
Mondal, Sumantra
Nanda, Amalesh
Bose, Shreyosi
Sanyal, Shairee
Jana, Saikat Kumar
Ghorai, Suvankar
author_facet Dutta, Reshmi
Thangapandi, K
Mondal, Sumantra
Nanda, Amalesh
Bose, Shreyosi
Sanyal, Shairee
Jana, Saikat Kumar
Ghorai, Suvankar
author_sort Dutta, Reshmi
collection PubMed
description BACKGROUND: Dengue burden is increasing day-by-day globally. A rapid, sensitive, cost-effective early diagnosis kit is the need of the hour. In this study, a label-free electrochemical immunosensor was proposed for dengue virus detection. A modified Polyaniline (PANI) coated Glassy Carbon (GC) electrode, immobilized with DENV NS1 antibody was used to detect the circulating DENV NS1 antigen in both spiked and infected sample. METHODS: Cloning, purification and expression of DENV NS1 protein in Escherichia coli (E. coli) was performed and sensor design, PANI modification on GC electrode surface by electrochemical polymerization and immobilization of NS1 antibody on the modified electrode surface was done and finally the analytical performance of the electrochemical immunosensor was done using Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS). RESULTS: CV and EIS were used to study and quantitate the circulating DENV antigen. The calibration curve showed wide linearity, good sensitivity (Slope=13.8% IpR/ml.ng( −1)) and distribution of data with a correlation coefficient (R) of 0.997. A lower Limit of Detection (LOD) was found to be 0.33 ng.ml( −1) which encourages the applicability of the sensor. CONCLUSION: Thus, a PANI based new electrochemical immunosensor has been developed which has the potential to be further modified for the development of cost effective, point of care dengue diagnostic kit.
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spelling pubmed-72294502020-05-19 Polyaniline Based Electrochemical Sensor for the Detection of Dengue Virus Infection Dutta, Reshmi Thangapandi, K Mondal, Sumantra Nanda, Amalesh Bose, Shreyosi Sanyal, Shairee Jana, Saikat Kumar Ghorai, Suvankar Avicenna J Med Biotechnol Original Article BACKGROUND: Dengue burden is increasing day-by-day globally. A rapid, sensitive, cost-effective early diagnosis kit is the need of the hour. In this study, a label-free electrochemical immunosensor was proposed for dengue virus detection. A modified Polyaniline (PANI) coated Glassy Carbon (GC) electrode, immobilized with DENV NS1 antibody was used to detect the circulating DENV NS1 antigen in both spiked and infected sample. METHODS: Cloning, purification and expression of DENV NS1 protein in Escherichia coli (E. coli) was performed and sensor design, PANI modification on GC electrode surface by electrochemical polymerization and immobilization of NS1 antibody on the modified electrode surface was done and finally the analytical performance of the electrochemical immunosensor was done using Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS). RESULTS: CV and EIS were used to study and quantitate the circulating DENV antigen. The calibration curve showed wide linearity, good sensitivity (Slope=13.8% IpR/ml.ng( −1)) and distribution of data with a correlation coefficient (R) of 0.997. A lower Limit of Detection (LOD) was found to be 0.33 ng.ml( −1) which encourages the applicability of the sensor. CONCLUSION: Thus, a PANI based new electrochemical immunosensor has been developed which has the potential to be further modified for the development of cost effective, point of care dengue diagnostic kit. Avicenna Research Institute 2020 /pmc/articles/PMC7229450/ /pubmed/32431791 Text en Copyright© 2020 Avicenna Research Institute http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Dutta, Reshmi
Thangapandi, K
Mondal, Sumantra
Nanda, Amalesh
Bose, Shreyosi
Sanyal, Shairee
Jana, Saikat Kumar
Ghorai, Suvankar
Polyaniline Based Electrochemical Sensor for the Detection of Dengue Virus Infection
title Polyaniline Based Electrochemical Sensor for the Detection of Dengue Virus Infection
title_full Polyaniline Based Electrochemical Sensor for the Detection of Dengue Virus Infection
title_fullStr Polyaniline Based Electrochemical Sensor for the Detection of Dengue Virus Infection
title_full_unstemmed Polyaniline Based Electrochemical Sensor for the Detection of Dengue Virus Infection
title_short Polyaniline Based Electrochemical Sensor for the Detection of Dengue Virus Infection
title_sort polyaniline based electrochemical sensor for the detection of dengue virus infection
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229450/
https://www.ncbi.nlm.nih.gov/pubmed/32431791
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