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In vivo and in vitro effects of Salsola collina on gastrointestinal motility in rats
OBJECTIVE(S): Salsola collina is widely distributed along the Bohai coast and consumed as an edible plant by native residents. We have found surprisingly that S. collina extracts promoted gastrointestinal motility in mice previously. In the present study, effects of S. collina on gastrointestinal mo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229513/ https://www.ncbi.nlm.nih.gov/pubmed/32440326 http://dx.doi.org/10.22038/IJBMS.2019.40613.9605 |
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author | Wang, Shasha Yan, Meixing Guo, Yaoyao Sun, Runzhou Jin, Hong Gong, Yanling |
author_facet | Wang, Shasha Yan, Meixing Guo, Yaoyao Sun, Runzhou Jin, Hong Gong, Yanling |
author_sort | Wang, Shasha |
collection | PubMed |
description | OBJECTIVE(S): Salsola collina is widely distributed along the Bohai coast and consumed as an edible plant by native residents. We have found surprisingly that S. collina extracts promoted gastrointestinal motility in mice previously. In the present study, effects of S. collina on gastrointestinal motility in rats and its underlying mechanism were explored. MATERIALS AND METHODS: In vivo, different fraction extracts from S. collina were prepared and the effects on gastric emptying and small intestinal propulsion in normal rats were measured. Plasma ghrelin (GRL), motilin (MTL), gastrin (GAS) and vasoactive intestinal peptide (VIP) and expressions of GRL receptor (GHSR), MTL receptor (MTLR), VIP receptor 2 (VIPR2) in the duodenum were also detected. In vitro, gastric antrum strips were prepared and activities of different extracts on gastric smooth muscle contractions were evaluated. RESULTS: Results showed that the ethyl acetate extract (EAE) was the most effective fraction to promote gastric emptying and intestinal propulsion, showing a dose-dependent manner. EAE increased plasma GRL and GAS, elevated GHSR expression and restrained VIPR2 expression in the duodenum. In vitro, EAE promoted contraction of normal gastric antrum strips as well as relaxed strips induced by atropine. CONCLUSION: These data indicate that EAE has a significant prokinetic activity via a mechanism that mainly involves in modulating plasma GRL and GAS, expressions of GSHR and VIPR2 in the duodenum and activating M-cholinergic receptor. Our study provides a pharmacological basis for the use of S. collina extract in treating gastrointestinal motility disorders. |
format | Online Article Text |
id | pubmed-7229513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-72295132020-05-21 In vivo and in vitro effects of Salsola collina on gastrointestinal motility in rats Wang, Shasha Yan, Meixing Guo, Yaoyao Sun, Runzhou Jin, Hong Gong, Yanling Iran J Basic Med Sci Original Article OBJECTIVE(S): Salsola collina is widely distributed along the Bohai coast and consumed as an edible plant by native residents. We have found surprisingly that S. collina extracts promoted gastrointestinal motility in mice previously. In the present study, effects of S. collina on gastrointestinal motility in rats and its underlying mechanism were explored. MATERIALS AND METHODS: In vivo, different fraction extracts from S. collina were prepared and the effects on gastric emptying and small intestinal propulsion in normal rats were measured. Plasma ghrelin (GRL), motilin (MTL), gastrin (GAS) and vasoactive intestinal peptide (VIP) and expressions of GRL receptor (GHSR), MTL receptor (MTLR), VIP receptor 2 (VIPR2) in the duodenum were also detected. In vitro, gastric antrum strips were prepared and activities of different extracts on gastric smooth muscle contractions were evaluated. RESULTS: Results showed that the ethyl acetate extract (EAE) was the most effective fraction to promote gastric emptying and intestinal propulsion, showing a dose-dependent manner. EAE increased plasma GRL and GAS, elevated GHSR expression and restrained VIPR2 expression in the duodenum. In vitro, EAE promoted contraction of normal gastric antrum strips as well as relaxed strips induced by atropine. CONCLUSION: These data indicate that EAE has a significant prokinetic activity via a mechanism that mainly involves in modulating plasma GRL and GAS, expressions of GSHR and VIPR2 in the duodenum and activating M-cholinergic receptor. Our study provides a pharmacological basis for the use of S. collina extract in treating gastrointestinal motility disorders. Mashhad University of Medical Sciences 2020-03 /pmc/articles/PMC7229513/ /pubmed/32440326 http://dx.doi.org/10.22038/IJBMS.2019.40613.9605 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Wang, Shasha Yan, Meixing Guo, Yaoyao Sun, Runzhou Jin, Hong Gong, Yanling In vivo and in vitro effects of Salsola collina on gastrointestinal motility in rats |
title |
In vivo and in vitro effects of Salsola collina on gastrointestinal motility in rats |
title_full |
In vivo and in vitro effects of Salsola collina on gastrointestinal motility in rats |
title_fullStr |
In vivo and in vitro effects of Salsola collina on gastrointestinal motility in rats |
title_full_unstemmed |
In vivo and in vitro effects of Salsola collina on gastrointestinal motility in rats |
title_short |
In vivo and in vitro effects of Salsola collina on gastrointestinal motility in rats |
title_sort | in vivo and in vitro effects of salsola collina on gastrointestinal motility in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229513/ https://www.ncbi.nlm.nih.gov/pubmed/32440326 http://dx.doi.org/10.22038/IJBMS.2019.40613.9605 |
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