Development of Screen-Printed Electrode Biosensor for Rapid Determination of Triglyceride Content in Coconut Milk

The screen-printed electrode biosensor was developed for triglyceride determination in coconut milk. The biosensor was developed by adding lipase, glycerol-3-phosphate (GPO), and glycerol kinase (GK), which is immobilized to a gelatin solution. The concentration of triglyceride is found to be linear...

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Detalles Bibliográficos
Autores principales: Manoj, D., Auddy, Ishita, Nimbkar, Shubham, Chittibabu, S., Shanmugasundaram, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229538/
https://www.ncbi.nlm.nih.gov/pubmed/32455128
http://dx.doi.org/10.1155/2020/1696201
Descripción
Sumario:The screen-printed electrode biosensor was developed for triglyceride determination in coconut milk. The biosensor was developed by adding lipase, glycerol-3-phosphate (GPO), and glycerol kinase (GK), which is immobilized to a gelatin solution. The concentration of triglyceride is found to be linear to the current produced. The developed screen-printed electrode biosensor showed the optimum response for pH 7.0, 45 mg amount of gelatin, 2.5% glutaraldehyde concentration solution. The developed biosensor was able to find triolein concentrations 0.1 to 1.5 mM. The correlation obtained between these two methods was 93% which was found to be good.

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