Development of Screen-Printed Electrode Biosensor for Rapid Determination of Triglyceride Content in Coconut Milk

The screen-printed electrode biosensor was developed for triglyceride determination in coconut milk. The biosensor was developed by adding lipase, glycerol-3-phosphate (GPO), and glycerol kinase (GK), which is immobilized to a gelatin solution. The concentration of triglyceride is found to be linear...

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Autores principales: Manoj, D., Auddy, Ishita, Nimbkar, Shubham, Chittibabu, S., Shanmugasundaram, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229538/
https://www.ncbi.nlm.nih.gov/pubmed/32455128
http://dx.doi.org/10.1155/2020/1696201
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author Manoj, D.
Auddy, Ishita
Nimbkar, Shubham
Chittibabu, S.
Shanmugasundaram, S.
author_facet Manoj, D.
Auddy, Ishita
Nimbkar, Shubham
Chittibabu, S.
Shanmugasundaram, S.
author_sort Manoj, D.
collection PubMed
description The screen-printed electrode biosensor was developed for triglyceride determination in coconut milk. The biosensor was developed by adding lipase, glycerol-3-phosphate (GPO), and glycerol kinase (GK), which is immobilized to a gelatin solution. The concentration of triglyceride is found to be linear to the current produced. The developed screen-printed electrode biosensor showed the optimum response for pH 7.0, 45 mg amount of gelatin, 2.5% glutaraldehyde concentration solution. The developed biosensor was able to find triolein concentrations 0.1 to 1.5 mM. The correlation obtained between these two methods was 93% which was found to be good.
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spelling pubmed-72295382020-05-23 Development of Screen-Printed Electrode Biosensor for Rapid Determination of Triglyceride Content in Coconut Milk Manoj, D. Auddy, Ishita Nimbkar, Shubham Chittibabu, S. Shanmugasundaram, S. Int J Food Sci Research Article The screen-printed electrode biosensor was developed for triglyceride determination in coconut milk. The biosensor was developed by adding lipase, glycerol-3-phosphate (GPO), and glycerol kinase (GK), which is immobilized to a gelatin solution. The concentration of triglyceride is found to be linear to the current produced. The developed screen-printed electrode biosensor showed the optimum response for pH 7.0, 45 mg amount of gelatin, 2.5% glutaraldehyde concentration solution. The developed biosensor was able to find triolein concentrations 0.1 to 1.5 mM. The correlation obtained between these two methods was 93% which was found to be good. Hindawi 2020-05-06 /pmc/articles/PMC7229538/ /pubmed/32455128 http://dx.doi.org/10.1155/2020/1696201 Text en Copyright © 2020 D. Manoj et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Manoj, D.
Auddy, Ishita
Nimbkar, Shubham
Chittibabu, S.
Shanmugasundaram, S.
Development of Screen-Printed Electrode Biosensor for Rapid Determination of Triglyceride Content in Coconut Milk
title Development of Screen-Printed Electrode Biosensor for Rapid Determination of Triglyceride Content in Coconut Milk
title_full Development of Screen-Printed Electrode Biosensor for Rapid Determination of Triglyceride Content in Coconut Milk
title_fullStr Development of Screen-Printed Electrode Biosensor for Rapid Determination of Triglyceride Content in Coconut Milk
title_full_unstemmed Development of Screen-Printed Electrode Biosensor for Rapid Determination of Triglyceride Content in Coconut Milk
title_short Development of Screen-Printed Electrode Biosensor for Rapid Determination of Triglyceride Content in Coconut Milk
title_sort development of screen-printed electrode biosensor for rapid determination of triglyceride content in coconut milk
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229538/
https://www.ncbi.nlm.nih.gov/pubmed/32455128
http://dx.doi.org/10.1155/2020/1696201
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