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Plasticity of nuclear and cytoplasmic stress responses of RNA-binding proteins
Cellular stress causes multifaceted reactions to trigger adaptive responses to environmental cues at all levels of the gene expression pathway. RNA-binding proteins (RBP) are key contributors to stress-induced regulation of RNA fate and function. Here, we uncover the plasticity of the RNA interactom...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229827/ https://www.ncbi.nlm.nih.gov/pubmed/32313943 http://dx.doi.org/10.1093/nar/gkaa256 |
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author | Backlund, Michael Stein, Frank Rettel, Mandy Schwarzl, Thomas Perez-Perri, Joel I Brosig, Annika Zhou, Yang Neu-Yilik, Gabriele Hentze, Matthias W Kulozik, Andreas E |
author_facet | Backlund, Michael Stein, Frank Rettel, Mandy Schwarzl, Thomas Perez-Perri, Joel I Brosig, Annika Zhou, Yang Neu-Yilik, Gabriele Hentze, Matthias W Kulozik, Andreas E |
author_sort | Backlund, Michael |
collection | PubMed |
description | Cellular stress causes multifaceted reactions to trigger adaptive responses to environmental cues at all levels of the gene expression pathway. RNA-binding proteins (RBP) are key contributors to stress-induced regulation of RNA fate and function. Here, we uncover the plasticity of the RNA interactome in stressed cells, differentiating between responses in the nucleus and in the cytoplasm. We applied enhanced RNA interactome capture (eRIC) analysis preceded by nucleo-cytoplasmic fractionation following arsenite-induced oxidative stress. The data reveal unexpectedly compartmentalized RNA interactomes and their responses to stress, including differential responses of RBPs in the nucleus versus the cytoplasm, which would have been missed by whole cell analyses. |
format | Online Article Text |
id | pubmed-7229827 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-72298272020-05-21 Plasticity of nuclear and cytoplasmic stress responses of RNA-binding proteins Backlund, Michael Stein, Frank Rettel, Mandy Schwarzl, Thomas Perez-Perri, Joel I Brosig, Annika Zhou, Yang Neu-Yilik, Gabriele Hentze, Matthias W Kulozik, Andreas E Nucleic Acids Res Data Resources and Analyses Cellular stress causes multifaceted reactions to trigger adaptive responses to environmental cues at all levels of the gene expression pathway. RNA-binding proteins (RBP) are key contributors to stress-induced regulation of RNA fate and function. Here, we uncover the plasticity of the RNA interactome in stressed cells, differentiating between responses in the nucleus and in the cytoplasm. We applied enhanced RNA interactome capture (eRIC) analysis preceded by nucleo-cytoplasmic fractionation following arsenite-induced oxidative stress. The data reveal unexpectedly compartmentalized RNA interactomes and their responses to stress, including differential responses of RBPs in the nucleus versus the cytoplasm, which would have been missed by whole cell analyses. Oxford University Press 2020-05-21 2020-04-20 /pmc/articles/PMC7229827/ /pubmed/32313943 http://dx.doi.org/10.1093/nar/gkaa256 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Data Resources and Analyses Backlund, Michael Stein, Frank Rettel, Mandy Schwarzl, Thomas Perez-Perri, Joel I Brosig, Annika Zhou, Yang Neu-Yilik, Gabriele Hentze, Matthias W Kulozik, Andreas E Plasticity of nuclear and cytoplasmic stress responses of RNA-binding proteins |
title | Plasticity of nuclear and cytoplasmic stress responses of RNA-binding proteins |
title_full | Plasticity of nuclear and cytoplasmic stress responses of RNA-binding proteins |
title_fullStr | Plasticity of nuclear and cytoplasmic stress responses of RNA-binding proteins |
title_full_unstemmed | Plasticity of nuclear and cytoplasmic stress responses of RNA-binding proteins |
title_short | Plasticity of nuclear and cytoplasmic stress responses of RNA-binding proteins |
title_sort | plasticity of nuclear and cytoplasmic stress responses of rna-binding proteins |
topic | Data Resources and Analyses |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229827/ https://www.ncbi.nlm.nih.gov/pubmed/32313943 http://dx.doi.org/10.1093/nar/gkaa256 |
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