Quantitative analysis of transcription factor binding and expression using calling cards reporter arrays

We report a tool, Calling Cards Reporter Arrays (CCRA), that measures transcription factor (TF) binding and the consequences on gene expression for hundreds of synthetic promoters in yeast. Using Cbf1p and MAX, we demonstrate that the CCRA method is able to detect small changes in binding free energ...

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Detalles Bibliográficos
Autores principales: Liu, Jiayue, Shively, Christian A, Mitra, Robi D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229839/
https://www.ncbi.nlm.nih.gov/pubmed/32133534
http://dx.doi.org/10.1093/nar/gkaa141

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229839/
https://www.ncbi.nlm.nih.gov/pubmed/32133534
http://dx.doi.org/10.1093/nar/gkaa141

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