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PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage

In recent years, CRISPR-associated (Cas) nucleases have revolutionized the genome editing field. Being guided by an RNA to cleave double-stranded (ds) DNA targets near a short sequence termed a protospacer adjacent motif (PAM), Cas9 and Cas12 offer unprecedented flexibility, however, more compact ve...

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Detalles Bibliográficos
Autores principales:	Karvelis, Tautvydas, Bigelyte, Greta, Young, Joshua K, Hou, Zhenglin, Zedaveinyte, Rimante, Budre, Karolina, Paulraj, Sushmitha, Djukanovic, Vesna, Gasior, Stephen, Silanskas, Arunas, Venclovas, Česlovas, Siksnys, Virginijus
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229846/ https://www.ncbi.nlm.nih.gov/pubmed/32246713 http://dx.doi.org/10.1093/nar/gkaa208

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