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Preliminary studies of hair follicle regeneration by injections of epidermal stem cells and dermal papilla cells into nude mice

The ultimate goal of organ regenerative therapy is to reproduce fully functional organs to replace which have been damaged as a result of diseases or injury. Although several studies claimed that using different types of cells in some animal models promote hair follicles regeneration, more researche...

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Autores principales: Zhang, Mingsheng, Ye, Yan, Zhao, Pin, Bai, Liming, Li, Xinping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230069/
https://www.ncbi.nlm.nih.gov/pubmed/32162163
http://dx.doi.org/10.1007/s10561-020-09825-4
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author Zhang, Mingsheng
Ye, Yan
Zhao, Pin
Bai, Liming
Li, Xinping
author_facet Zhang, Mingsheng
Ye, Yan
Zhao, Pin
Bai, Liming
Li, Xinping
author_sort Zhang, Mingsheng
collection PubMed
description The ultimate goal of organ regenerative therapy is to reproduce fully functional organs to replace which have been damaged as a result of diseases or injury. Although several studies claimed that using different types of cells in some animal models promote hair follicles regeneration, more researches can be done to develop a sufficient and efficient protocol to induce hair generation from different animal models. In this study, we investigated the therapeutic potentials for hair follicle formation by injecting a mixture of epidermal stem cells and dermal papilla cells. Those cells were isolated and culture-expanded. Then we randomly allocated 8 nude mice into two groups. The experiment group received an injection of a mixture that containing of epidermal stem cells and dermal papilla cells. The control group received injection of keratinocyte serum-free medium. The hair follicles regeneration was observed and the injection area was harvested for HE staining. 14 day later, the regenerated hair shafts were observed and HE staining indicated that the newly hair follicle formed the correct structures in experiment group. Furthermore, the mixture injection induced a regular and multilayered stratified epidermis and the epidermis contained of hair follicle-likes structures. Our data showed that injection of a mixture of epidermal stem cells and dermal papilla cells could induce hair follicles regeneration and well-ordered epidermis formation. This study emphasized that the rearrangement of the interactions during seed cells and the niches of the seed cells is essential and necessary for tissue-engineered construct success.
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spelling pubmed-72300692020-05-18 Preliminary studies of hair follicle regeneration by injections of epidermal stem cells and dermal papilla cells into nude mice Zhang, Mingsheng Ye, Yan Zhao, Pin Bai, Liming Li, Xinping Cell Tissue Bank Full Length Paper The ultimate goal of organ regenerative therapy is to reproduce fully functional organs to replace which have been damaged as a result of diseases or injury. Although several studies claimed that using different types of cells in some animal models promote hair follicles regeneration, more researches can be done to develop a sufficient and efficient protocol to induce hair generation from different animal models. In this study, we investigated the therapeutic potentials for hair follicle formation by injecting a mixture of epidermal stem cells and dermal papilla cells. Those cells were isolated and culture-expanded. Then we randomly allocated 8 nude mice into two groups. The experiment group received an injection of a mixture that containing of epidermal stem cells and dermal papilla cells. The control group received injection of keratinocyte serum-free medium. The hair follicles regeneration was observed and the injection area was harvested for HE staining. 14 day later, the regenerated hair shafts were observed and HE staining indicated that the newly hair follicle formed the correct structures in experiment group. Furthermore, the mixture injection induced a regular and multilayered stratified epidermis and the epidermis contained of hair follicle-likes structures. Our data showed that injection of a mixture of epidermal stem cells and dermal papilla cells could induce hair follicles regeneration and well-ordered epidermis formation. This study emphasized that the rearrangement of the interactions during seed cells and the niches of the seed cells is essential and necessary for tissue-engineered construct success. Springer Netherlands 2020-03-11 2020 /pmc/articles/PMC7230069/ /pubmed/32162163 http://dx.doi.org/10.1007/s10561-020-09825-4 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Full Length Paper
Zhang, Mingsheng
Ye, Yan
Zhao, Pin
Bai, Liming
Li, Xinping
Preliminary studies of hair follicle regeneration by injections of epidermal stem cells and dermal papilla cells into nude mice
title Preliminary studies of hair follicle regeneration by injections of epidermal stem cells and dermal papilla cells into nude mice
title_full Preliminary studies of hair follicle regeneration by injections of epidermal stem cells and dermal papilla cells into nude mice
title_fullStr Preliminary studies of hair follicle regeneration by injections of epidermal stem cells and dermal papilla cells into nude mice
title_full_unstemmed Preliminary studies of hair follicle regeneration by injections of epidermal stem cells and dermal papilla cells into nude mice
title_short Preliminary studies of hair follicle regeneration by injections of epidermal stem cells and dermal papilla cells into nude mice
title_sort preliminary studies of hair follicle regeneration by injections of epidermal stem cells and dermal papilla cells into nude mice
topic Full Length Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230069/
https://www.ncbi.nlm.nih.gov/pubmed/32162163
http://dx.doi.org/10.1007/s10561-020-09825-4
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