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The Role of VEGFA, COX2, HUR and CUGBP2 in Predicting the Response to Neoadjuvant Therapy in Rectal Cancer Patients

Background and objectives: The effectiveness of neoadjuvant therapy, which is commonly used for stage II-III rectal cancer (RC) treatment, is limited. Genes associated with the pathogenesis of RC could determine response to this treatment. Therefore, the aim of this study was to investigate the pote...

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Autores principales: Pauzas, Henrikas, Gyvyte, Ugne, Latkauskas, Tadas, Kairevice, Laura, Lizdenis, Paulius, Svagzdys, Saulius, Birgiolaite, Erika, Kuliaviene, Irma, Kupcinskas, Juozas, Tamelis, Algimantas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230171/
https://www.ncbi.nlm.nih.gov/pubmed/32331433
http://dx.doi.org/10.3390/medicina56040192
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author Pauzas, Henrikas
Gyvyte, Ugne
Latkauskas, Tadas
Kairevice, Laura
Lizdenis, Paulius
Svagzdys, Saulius
Birgiolaite, Erika
Kuliaviene, Irma
Kupcinskas, Juozas
Tamelis, Algimantas
author_facet Pauzas, Henrikas
Gyvyte, Ugne
Latkauskas, Tadas
Kairevice, Laura
Lizdenis, Paulius
Svagzdys, Saulius
Birgiolaite, Erika
Kuliaviene, Irma
Kupcinskas, Juozas
Tamelis, Algimantas
author_sort Pauzas, Henrikas
collection PubMed
description Background and objectives: The effectiveness of neoadjuvant therapy, which is commonly used for stage II-III rectal cancer (RC) treatment, is limited. Genes associated with the pathogenesis of RC could determine response to this treatment. Therefore, the aim of this study was to investigate the potential predictive value of VEGFA, COX2, HUR and CUGBP2 genes and the associations between post-treatment changes in gene expression and the efficacy of neoadjuvant therapy. Materials and Methods: Biopsies from RC and healthy rectal tissue of 28 RC patients were collected before neoadjuvant therapy and 6-8 weeks after neoadjuvant therapy. The expression levels of VEGFA, COX2, HUR, CUGBP2 genes were evaluated using a quantitative real-time polymerase chain reaction. Results: The results reveal a significantly higher expression of VEGFA, COX2 and HUR mRNA in RC tissue compared to healthy rectal tissue (p < 0.05), and elevated VEGFA gene expression in pre-treatment tissues was associated with a better response to neoadjuvant therapy based on T-stage downstaging (p < 0.05). The expression of VEGFA, HUR and CUGBP2 genes significantly decreased after neoadjuvant therapy (p < 0.05). Responders to treatment demonstrated a significantly stronger decrease of VEGFA and COX2 expression after neoadjuvant therapy than non-responders (p < 0.05). Conclusions: The findings of this study suggest that the pre-treatment VEGFA gene expression might have predictive value for the response to neoadjuvant therapy, while the post-treatment decrease in VEGFA and COX2 gene expression could indicate the effectiveness of neoadjuvant therapy in RC patients.
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spelling pubmed-72301712020-05-28 The Role of VEGFA, COX2, HUR and CUGBP2 in Predicting the Response to Neoadjuvant Therapy in Rectal Cancer Patients Pauzas, Henrikas Gyvyte, Ugne Latkauskas, Tadas Kairevice, Laura Lizdenis, Paulius Svagzdys, Saulius Birgiolaite, Erika Kuliaviene, Irma Kupcinskas, Juozas Tamelis, Algimantas Medicina (Kaunas) Article Background and objectives: The effectiveness of neoadjuvant therapy, which is commonly used for stage II-III rectal cancer (RC) treatment, is limited. Genes associated with the pathogenesis of RC could determine response to this treatment. Therefore, the aim of this study was to investigate the potential predictive value of VEGFA, COX2, HUR and CUGBP2 genes and the associations between post-treatment changes in gene expression and the efficacy of neoadjuvant therapy. Materials and Methods: Biopsies from RC and healthy rectal tissue of 28 RC patients were collected before neoadjuvant therapy and 6-8 weeks after neoadjuvant therapy. The expression levels of VEGFA, COX2, HUR, CUGBP2 genes were evaluated using a quantitative real-time polymerase chain reaction. Results: The results reveal a significantly higher expression of VEGFA, COX2 and HUR mRNA in RC tissue compared to healthy rectal tissue (p < 0.05), and elevated VEGFA gene expression in pre-treatment tissues was associated with a better response to neoadjuvant therapy based on T-stage downstaging (p < 0.05). The expression of VEGFA, HUR and CUGBP2 genes significantly decreased after neoadjuvant therapy (p < 0.05). Responders to treatment demonstrated a significantly stronger decrease of VEGFA and COX2 expression after neoadjuvant therapy than non-responders (p < 0.05). Conclusions: The findings of this study suggest that the pre-treatment VEGFA gene expression might have predictive value for the response to neoadjuvant therapy, while the post-treatment decrease in VEGFA and COX2 gene expression could indicate the effectiveness of neoadjuvant therapy in RC patients. MDPI 2020-04-22 /pmc/articles/PMC7230171/ /pubmed/32331433 http://dx.doi.org/10.3390/medicina56040192 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pauzas, Henrikas
Gyvyte, Ugne
Latkauskas, Tadas
Kairevice, Laura
Lizdenis, Paulius
Svagzdys, Saulius
Birgiolaite, Erika
Kuliaviene, Irma
Kupcinskas, Juozas
Tamelis, Algimantas
The Role of VEGFA, COX2, HUR and CUGBP2 in Predicting the Response to Neoadjuvant Therapy in Rectal Cancer Patients
title The Role of VEGFA, COX2, HUR and CUGBP2 in Predicting the Response to Neoadjuvant Therapy in Rectal Cancer Patients
title_full The Role of VEGFA, COX2, HUR and CUGBP2 in Predicting the Response to Neoadjuvant Therapy in Rectal Cancer Patients
title_fullStr The Role of VEGFA, COX2, HUR and CUGBP2 in Predicting the Response to Neoadjuvant Therapy in Rectal Cancer Patients
title_full_unstemmed The Role of VEGFA, COX2, HUR and CUGBP2 in Predicting the Response to Neoadjuvant Therapy in Rectal Cancer Patients
title_short The Role of VEGFA, COX2, HUR and CUGBP2 in Predicting the Response to Neoadjuvant Therapy in Rectal Cancer Patients
title_sort role of vegfa, cox2, hur and cugbp2 in predicting the response to neoadjuvant therapy in rectal cancer patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230171/
https://www.ncbi.nlm.nih.gov/pubmed/32331433
http://dx.doi.org/10.3390/medicina56040192
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