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Sickle Cell Disease: Metabolomic Profiles of Vaso-Occlusive Crisis in Plasma and Erythrocytes

The metabolomic profile of vaso-occlusive crisis, compared to the basal state of sickle cell disease, has never been reported to our knowledge. Using a standardized targeted metabolomic approach, performed on plasma and erythrocyte fractions, we compared these two states of the disease in the same g...

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Autores principales: Dembélé, Klétigui Casimir, Veyrat-Durebex, Charlotte, Guindo, Aldiouma, Chupin, Stéphanie, Tessier, Lydie, Goïta, Yaya, Baraïka, Mohamed Ag, Diallo, Moussa, Touré, Boubacari Ali, Homedan, Chadi, Mirebeau-Prunier, Delphine, Simard, Gilles, Diallo, Dapa, Cissé, Bakary Mamadou, Reynier, Pascal, Chao de la Barca, Juan Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230294/
https://www.ncbi.nlm.nih.gov/pubmed/32290473
http://dx.doi.org/10.3390/jcm9041092
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author Dembélé, Klétigui Casimir
Veyrat-Durebex, Charlotte
Guindo, Aldiouma
Chupin, Stéphanie
Tessier, Lydie
Goïta, Yaya
Baraïka, Mohamed Ag
Diallo, Moussa
Touré, Boubacari Ali
Homedan, Chadi
Mirebeau-Prunier, Delphine
Simard, Gilles
Diallo, Dapa
Cissé, Bakary Mamadou
Reynier, Pascal
Chao de la Barca, Juan Manuel
author_facet Dembélé, Klétigui Casimir
Veyrat-Durebex, Charlotte
Guindo, Aldiouma
Chupin, Stéphanie
Tessier, Lydie
Goïta, Yaya
Baraïka, Mohamed Ag
Diallo, Moussa
Touré, Boubacari Ali
Homedan, Chadi
Mirebeau-Prunier, Delphine
Simard, Gilles
Diallo, Dapa
Cissé, Bakary Mamadou
Reynier, Pascal
Chao de la Barca, Juan Manuel
author_sort Dembélé, Klétigui Casimir
collection PubMed
description The metabolomic profile of vaso-occlusive crisis, compared to the basal state of sickle cell disease, has never been reported to our knowledge. Using a standardized targeted metabolomic approach, performed on plasma and erythrocyte fractions, we compared these two states of the disease in the same group of 40 patients. Among the 188 metabolites analyzed, 153 were accurately measured in plasma and 143 in red blood cells. Supervised paired partial least squares discriminant analysis (pPLS-DA) showed good predictive performance for test sets with median area under the receiver operating characteristic (AUROC) curves of 99% and mean p-values of 0.0005 and 0.0002 in plasma and erythrocytes, respectively. A total of 63 metabolites allowed discrimination between the two groups in the plasma, whereas 61 allowed discrimination in the erythrocytes. Overall, this signature points to altered arginine and nitric oxide metabolism, pain pathophysiology, hypoxia and energetic crisis, and membrane remodeling of red blood cells. It also revealed the alteration of metabolite concentrations that had not been previously associated with sickle cell disease. Our results demonstrate that the vaso-occlusive crisis has a specific metabolomic signature, distinct from that observed at steady state, which may be potentially helpful for finding predictive biomarkers for this acute life-threatening episode.
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spelling pubmed-72302942020-05-22 Sickle Cell Disease: Metabolomic Profiles of Vaso-Occlusive Crisis in Plasma and Erythrocytes Dembélé, Klétigui Casimir Veyrat-Durebex, Charlotte Guindo, Aldiouma Chupin, Stéphanie Tessier, Lydie Goïta, Yaya Baraïka, Mohamed Ag Diallo, Moussa Touré, Boubacari Ali Homedan, Chadi Mirebeau-Prunier, Delphine Simard, Gilles Diallo, Dapa Cissé, Bakary Mamadou Reynier, Pascal Chao de la Barca, Juan Manuel J Clin Med Article The metabolomic profile of vaso-occlusive crisis, compared to the basal state of sickle cell disease, has never been reported to our knowledge. Using a standardized targeted metabolomic approach, performed on plasma and erythrocyte fractions, we compared these two states of the disease in the same group of 40 patients. Among the 188 metabolites analyzed, 153 were accurately measured in plasma and 143 in red blood cells. Supervised paired partial least squares discriminant analysis (pPLS-DA) showed good predictive performance for test sets with median area under the receiver operating characteristic (AUROC) curves of 99% and mean p-values of 0.0005 and 0.0002 in plasma and erythrocytes, respectively. A total of 63 metabolites allowed discrimination between the two groups in the plasma, whereas 61 allowed discrimination in the erythrocytes. Overall, this signature points to altered arginine and nitric oxide metabolism, pain pathophysiology, hypoxia and energetic crisis, and membrane remodeling of red blood cells. It also revealed the alteration of metabolite concentrations that had not been previously associated with sickle cell disease. Our results demonstrate that the vaso-occlusive crisis has a specific metabolomic signature, distinct from that observed at steady state, which may be potentially helpful for finding predictive biomarkers for this acute life-threatening episode. MDPI 2020-04-11 /pmc/articles/PMC7230294/ /pubmed/32290473 http://dx.doi.org/10.3390/jcm9041092 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dembélé, Klétigui Casimir
Veyrat-Durebex, Charlotte
Guindo, Aldiouma
Chupin, Stéphanie
Tessier, Lydie
Goïta, Yaya
Baraïka, Mohamed Ag
Diallo, Moussa
Touré, Boubacari Ali
Homedan, Chadi
Mirebeau-Prunier, Delphine
Simard, Gilles
Diallo, Dapa
Cissé, Bakary Mamadou
Reynier, Pascal
Chao de la Barca, Juan Manuel
Sickle Cell Disease: Metabolomic Profiles of Vaso-Occlusive Crisis in Plasma and Erythrocytes
title Sickle Cell Disease: Metabolomic Profiles of Vaso-Occlusive Crisis in Plasma and Erythrocytes
title_full Sickle Cell Disease: Metabolomic Profiles of Vaso-Occlusive Crisis in Plasma and Erythrocytes
title_fullStr Sickle Cell Disease: Metabolomic Profiles of Vaso-Occlusive Crisis in Plasma and Erythrocytes
title_full_unstemmed Sickle Cell Disease: Metabolomic Profiles of Vaso-Occlusive Crisis in Plasma and Erythrocytes
title_short Sickle Cell Disease: Metabolomic Profiles of Vaso-Occlusive Crisis in Plasma and Erythrocytes
title_sort sickle cell disease: metabolomic profiles of vaso-occlusive crisis in plasma and erythrocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230294/
https://www.ncbi.nlm.nih.gov/pubmed/32290473
http://dx.doi.org/10.3390/jcm9041092
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