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2D Spatially-Resolved Depth-Section Microfluidic Flow Velocimetry Using Dual Beam OCT

A dual beam optical coherence tomography (OCT) instrument has been developed for flow measurement that offers advantages over microscope derived imaging techniques. It requires only a single optical access port, allows simultaneous imaging of the microfluidic channel, does not require fluorescent se...

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Detalles Bibliográficos
Autores principales: Hallam, Jonathan M., Rigas, Evangelos, Charrett, Thomas O. H., Tatam, Ralph P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230295/
https://www.ncbi.nlm.nih.gov/pubmed/32230993
http://dx.doi.org/10.3390/mi11040351
Descripción
Sumario:A dual beam optical coherence tomography (OCT) instrument has been developed for flow measurement that offers advantages over microscope derived imaging techniques. It requires only a single optical access port, allows simultaneous imaging of the microfluidic channel, does not require fluorescent seed particles, and can provide a millimetre-deep depth-section velocity profile (as opposed to horizontal-section). The dual beam instrument performs rapid re-sampling of particle positions, allowing measurement of faster flows. In this paper, we develop the methods and processes necessary to make 2D quantitative measurements of the flow-velocity using dual beam OCT and present exemplar results in a microfluidic chip. A 2D reference measurement of the Poiseuille flow in a microfluidic channel is presented over a spanwise depth range of 700 μm and streamwise length of 1600 μm with a spatial resolution of 10 μm, at velocities up to 50 mm/s. A measurement of a more complex flow field is also demonstrated in a sloped microfluidic section.