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2D Spatially-Resolved Depth-Section Microfluidic Flow Velocimetry Using Dual Beam OCT
A dual beam optical coherence tomography (OCT) instrument has been developed for flow measurement that offers advantages over microscope derived imaging techniques. It requires only a single optical access port, allows simultaneous imaging of the microfluidic channel, does not require fluorescent se...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230295/ https://www.ncbi.nlm.nih.gov/pubmed/32230993 http://dx.doi.org/10.3390/mi11040351 |
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author | Hallam, Jonathan M. Rigas, Evangelos Charrett, Thomas O. H. Tatam, Ralph P. |
author_facet | Hallam, Jonathan M. Rigas, Evangelos Charrett, Thomas O. H. Tatam, Ralph P. |
author_sort | Hallam, Jonathan M. |
collection | PubMed |
description | A dual beam optical coherence tomography (OCT) instrument has been developed for flow measurement that offers advantages over microscope derived imaging techniques. It requires only a single optical access port, allows simultaneous imaging of the microfluidic channel, does not require fluorescent seed particles, and can provide a millimetre-deep depth-section velocity profile (as opposed to horizontal-section). The dual beam instrument performs rapid re-sampling of particle positions, allowing measurement of faster flows. In this paper, we develop the methods and processes necessary to make 2D quantitative measurements of the flow-velocity using dual beam OCT and present exemplar results in a microfluidic chip. A 2D reference measurement of the Poiseuille flow in a microfluidic channel is presented over a spanwise depth range of 700 μm and streamwise length of 1600 μm with a spatial resolution of 10 μm, at velocities up to 50 mm/s. A measurement of a more complex flow field is also demonstrated in a sloped microfluidic section. |
format | Online Article Text |
id | pubmed-7230295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72302952020-05-22 2D Spatially-Resolved Depth-Section Microfluidic Flow Velocimetry Using Dual Beam OCT Hallam, Jonathan M. Rigas, Evangelos Charrett, Thomas O. H. Tatam, Ralph P. Micromachines (Basel) Article A dual beam optical coherence tomography (OCT) instrument has been developed for flow measurement that offers advantages over microscope derived imaging techniques. It requires only a single optical access port, allows simultaneous imaging of the microfluidic channel, does not require fluorescent seed particles, and can provide a millimetre-deep depth-section velocity profile (as opposed to horizontal-section). The dual beam instrument performs rapid re-sampling of particle positions, allowing measurement of faster flows. In this paper, we develop the methods and processes necessary to make 2D quantitative measurements of the flow-velocity using dual beam OCT and present exemplar results in a microfluidic chip. A 2D reference measurement of the Poiseuille flow in a microfluidic channel is presented over a spanwise depth range of 700 μm and streamwise length of 1600 μm with a spatial resolution of 10 μm, at velocities up to 50 mm/s. A measurement of a more complex flow field is also demonstrated in a sloped microfluidic section. MDPI 2020-03-27 /pmc/articles/PMC7230295/ /pubmed/32230993 http://dx.doi.org/10.3390/mi11040351 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hallam, Jonathan M. Rigas, Evangelos Charrett, Thomas O. H. Tatam, Ralph P. 2D Spatially-Resolved Depth-Section Microfluidic Flow Velocimetry Using Dual Beam OCT |
title | 2D Spatially-Resolved Depth-Section Microfluidic Flow Velocimetry Using Dual Beam OCT |
title_full | 2D Spatially-Resolved Depth-Section Microfluidic Flow Velocimetry Using Dual Beam OCT |
title_fullStr | 2D Spatially-Resolved Depth-Section Microfluidic Flow Velocimetry Using Dual Beam OCT |
title_full_unstemmed | 2D Spatially-Resolved Depth-Section Microfluidic Flow Velocimetry Using Dual Beam OCT |
title_short | 2D Spatially-Resolved Depth-Section Microfluidic Flow Velocimetry Using Dual Beam OCT |
title_sort | 2d spatially-resolved depth-section microfluidic flow velocimetry using dual beam oct |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230295/ https://www.ncbi.nlm.nih.gov/pubmed/32230993 http://dx.doi.org/10.3390/mi11040351 |
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