Cargando…
Low Levels of Serum Tryptophan Underlie Skeletal Muscle Atrophy
Sarcopenia is a poor prognosis factor in some cancer patients, but little is known about the mechanisms by which malignant tumors cause skeletal muscle atrophy. Tryptophan metabolism mediated by indoleamine 2,3-dioxygenase is one of the most important amino acid changes associated with cancer progre...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230402/ https://www.ncbi.nlm.nih.gov/pubmed/32244785 http://dx.doi.org/10.3390/nu12040978 |
_version_ | 1783534941819109376 |
---|---|
author | Ninomiya, Soranobu Nakamura, Nobuhiko Nakamura, Hiroshi Mizutani, Taku Kaneda, Yuto Yamaguchi, Kimihiro Matsumoto, Takuro Kitagawa, Junichi Kanemura, Nobuhiro Shiraki, Makoto Hara, Takeshi Shimizu, Masahito Tsurumi, Hisashi |
author_facet | Ninomiya, Soranobu Nakamura, Nobuhiko Nakamura, Hiroshi Mizutani, Taku Kaneda, Yuto Yamaguchi, Kimihiro Matsumoto, Takuro Kitagawa, Junichi Kanemura, Nobuhiro Shiraki, Makoto Hara, Takeshi Shimizu, Masahito Tsurumi, Hisashi |
author_sort | Ninomiya, Soranobu |
collection | PubMed |
description | Sarcopenia is a poor prognosis factor in some cancer patients, but little is known about the mechanisms by which malignant tumors cause skeletal muscle atrophy. Tryptophan metabolism mediated by indoleamine 2,3-dioxygenase is one of the most important amino acid changes associated with cancer progression. Herein, we demonstrate the relationship between skeletal muscles and low levels of tryptophan. A positive correlation was observed between the volume of skeletal muscles and serum tryptophan levels in patients with diffuse large B-cell lymphoma. Low levels of tryptophan reduced C2C12 myoblast cell proliferation and differentiation. Fiber diameters in the tibialis anterior of C57BL/6 mice fed a tryptophan-deficient diet were smaller than those in mice fed a standard diet. Metabolomics analysis revealed that tryptophan-deficient diet downregulated glycolysis in the gastrocnemius and upregulated the concentrations of amino acids associated with the tricarboxylic acid cycle. The weights and muscle fiber diameters of mice fed the tryptophan-deficient diet recovered after switching to the standard diet. Our data showed a critical role for tryptophan in regulating skeletal muscle mass. Thus, the tryptophan metabolism pathway may be a promising target for preventing or treating skeletal muscle atrophies. |
format | Online Article Text |
id | pubmed-7230402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72304022020-05-22 Low Levels of Serum Tryptophan Underlie Skeletal Muscle Atrophy Ninomiya, Soranobu Nakamura, Nobuhiko Nakamura, Hiroshi Mizutani, Taku Kaneda, Yuto Yamaguchi, Kimihiro Matsumoto, Takuro Kitagawa, Junichi Kanemura, Nobuhiro Shiraki, Makoto Hara, Takeshi Shimizu, Masahito Tsurumi, Hisashi Nutrients Article Sarcopenia is a poor prognosis factor in some cancer patients, but little is known about the mechanisms by which malignant tumors cause skeletal muscle atrophy. Tryptophan metabolism mediated by indoleamine 2,3-dioxygenase is one of the most important amino acid changes associated with cancer progression. Herein, we demonstrate the relationship between skeletal muscles and low levels of tryptophan. A positive correlation was observed between the volume of skeletal muscles and serum tryptophan levels in patients with diffuse large B-cell lymphoma. Low levels of tryptophan reduced C2C12 myoblast cell proliferation and differentiation. Fiber diameters in the tibialis anterior of C57BL/6 mice fed a tryptophan-deficient diet were smaller than those in mice fed a standard diet. Metabolomics analysis revealed that tryptophan-deficient diet downregulated glycolysis in the gastrocnemius and upregulated the concentrations of amino acids associated with the tricarboxylic acid cycle. The weights and muscle fiber diameters of mice fed the tryptophan-deficient diet recovered after switching to the standard diet. Our data showed a critical role for tryptophan in regulating skeletal muscle mass. Thus, the tryptophan metabolism pathway may be a promising target for preventing or treating skeletal muscle atrophies. MDPI 2020-04-01 /pmc/articles/PMC7230402/ /pubmed/32244785 http://dx.doi.org/10.3390/nu12040978 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ninomiya, Soranobu Nakamura, Nobuhiko Nakamura, Hiroshi Mizutani, Taku Kaneda, Yuto Yamaguchi, Kimihiro Matsumoto, Takuro Kitagawa, Junichi Kanemura, Nobuhiro Shiraki, Makoto Hara, Takeshi Shimizu, Masahito Tsurumi, Hisashi Low Levels of Serum Tryptophan Underlie Skeletal Muscle Atrophy |
title | Low Levels of Serum Tryptophan Underlie Skeletal Muscle Atrophy |
title_full | Low Levels of Serum Tryptophan Underlie Skeletal Muscle Atrophy |
title_fullStr | Low Levels of Serum Tryptophan Underlie Skeletal Muscle Atrophy |
title_full_unstemmed | Low Levels of Serum Tryptophan Underlie Skeletal Muscle Atrophy |
title_short | Low Levels of Serum Tryptophan Underlie Skeletal Muscle Atrophy |
title_sort | low levels of serum tryptophan underlie skeletal muscle atrophy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230402/ https://www.ncbi.nlm.nih.gov/pubmed/32244785 http://dx.doi.org/10.3390/nu12040978 |
work_keys_str_mv | AT ninomiyasoranobu lowlevelsofserumtryptophanunderlieskeletalmuscleatrophy AT nakamuranobuhiko lowlevelsofserumtryptophanunderlieskeletalmuscleatrophy AT nakamurahiroshi lowlevelsofserumtryptophanunderlieskeletalmuscleatrophy AT mizutanitaku lowlevelsofserumtryptophanunderlieskeletalmuscleatrophy AT kanedayuto lowlevelsofserumtryptophanunderlieskeletalmuscleatrophy AT yamaguchikimihiro lowlevelsofserumtryptophanunderlieskeletalmuscleatrophy AT matsumototakuro lowlevelsofserumtryptophanunderlieskeletalmuscleatrophy AT kitagawajunichi lowlevelsofserumtryptophanunderlieskeletalmuscleatrophy AT kanemuranobuhiro lowlevelsofserumtryptophanunderlieskeletalmuscleatrophy AT shirakimakoto lowlevelsofserumtryptophanunderlieskeletalmuscleatrophy AT haratakeshi lowlevelsofserumtryptophanunderlieskeletalmuscleatrophy AT shimizumasahito lowlevelsofserumtryptophanunderlieskeletalmuscleatrophy AT tsurumihisashi lowlevelsofserumtryptophanunderlieskeletalmuscleatrophy |