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Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats

Fasting is known to cause physiological changes in the endocrine pancreas, including decreased insulin secretion and increased reactive oxygen species (ROS) production. However, there is no consensus about the long-term effects of intermittent fasting (IF), which can involve up to 24 hours of fastin...

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Autores principales: Munhoz, Ana Cláudia, Vilas-Boas, Eloisa Aparecida, Panveloski-Costa, Ana Carolina, Leite, Jaqueline Santos Moreira, Lucena, Camila Ferraz, Riva, Patrícia, Emilio, Henriette, Carpinelli, Angelo R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230500/
https://www.ncbi.nlm.nih.gov/pubmed/32283715
http://dx.doi.org/10.3390/nu12041029
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author Munhoz, Ana Cláudia
Vilas-Boas, Eloisa Aparecida
Panveloski-Costa, Ana Carolina
Leite, Jaqueline Santos Moreira
Lucena, Camila Ferraz
Riva, Patrícia
Emilio, Henriette
Carpinelli, Angelo R.
author_facet Munhoz, Ana Cláudia
Vilas-Boas, Eloisa Aparecida
Panveloski-Costa, Ana Carolina
Leite, Jaqueline Santos Moreira
Lucena, Camila Ferraz
Riva, Patrícia
Emilio, Henriette
Carpinelli, Angelo R.
author_sort Munhoz, Ana Cláudia
collection PubMed
description Fasting is known to cause physiological changes in the endocrine pancreas, including decreased insulin secretion and increased reactive oxygen species (ROS) production. However, there is no consensus about the long-term effects of intermittent fasting (IF), which can involve up to 24 hours of fasting interspersed with normal feeding days. In the present study, we analyzed the effects of alternate-day IF for 12 weeks in a developing and healthy organism. Female 30-day-old Wistar rats were randomly divided into two groups: control, with free access to standard rodent chow; and IF, subjected to 24-hour fasts intercalated with 24-hours of free access to the same chow. Alternate-day IF decreased weight gain and food intake. Surprisingly, IF also elevated plasma insulin concentrations, both at baseline and after glucose administration collected during oGTT. After 12 weeks of dietary intervention, pancreatic islets displayed increased ROS production and apoptosis. Despite their lower body weight, IF animals had increased fat reserves and decreased muscle mass. Taken together, these findings suggest that alternate-day IF promote β -cell dysfunction, especially in developing animals. More long-term research is necessary to define the best IF protocol to reduce side effects.
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spelling pubmed-72305002020-05-22 Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats Munhoz, Ana Cláudia Vilas-Boas, Eloisa Aparecida Panveloski-Costa, Ana Carolina Leite, Jaqueline Santos Moreira Lucena, Camila Ferraz Riva, Patrícia Emilio, Henriette Carpinelli, Angelo R. Nutrients Article Fasting is known to cause physiological changes in the endocrine pancreas, including decreased insulin secretion and increased reactive oxygen species (ROS) production. However, there is no consensus about the long-term effects of intermittent fasting (IF), which can involve up to 24 hours of fasting interspersed with normal feeding days. In the present study, we analyzed the effects of alternate-day IF for 12 weeks in a developing and healthy organism. Female 30-day-old Wistar rats were randomly divided into two groups: control, with free access to standard rodent chow; and IF, subjected to 24-hour fasts intercalated with 24-hours of free access to the same chow. Alternate-day IF decreased weight gain and food intake. Surprisingly, IF also elevated plasma insulin concentrations, both at baseline and after glucose administration collected during oGTT. After 12 weeks of dietary intervention, pancreatic islets displayed increased ROS production and apoptosis. Despite their lower body weight, IF animals had increased fat reserves and decreased muscle mass. Taken together, these findings suggest that alternate-day IF promote β -cell dysfunction, especially in developing animals. More long-term research is necessary to define the best IF protocol to reduce side effects. MDPI 2020-04-09 /pmc/articles/PMC7230500/ /pubmed/32283715 http://dx.doi.org/10.3390/nu12041029 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Munhoz, Ana Cláudia
Vilas-Boas, Eloisa Aparecida
Panveloski-Costa, Ana Carolina
Leite, Jaqueline Santos Moreira
Lucena, Camila Ferraz
Riva, Patrícia
Emilio, Henriette
Carpinelli, Angelo R.
Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats
title Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats
title_full Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats
title_fullStr Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats
title_full_unstemmed Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats
title_short Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats
title_sort intermittent fasting for twelve weeks leads to increases in fat mass and hyperinsulinemia in young female wistar rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230500/
https://www.ncbi.nlm.nih.gov/pubmed/32283715
http://dx.doi.org/10.3390/nu12041029
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