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Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels
Miniaturized quantitative assays offer multiplexing capability in a microfluidic device for high-throughput applications such as antimicrobial resistance (AMR) studies. The detection of these multiple microchannels in a single microfluidic device becomes crucial for point-of-care (POC) testing and c...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230666/ https://www.ncbi.nlm.nih.gov/pubmed/32260509 http://dx.doi.org/10.3390/mi11040385 |
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author | Hassan, Sammer-ul Zhang, Xunli |
author_facet | Hassan, Sammer-ul Zhang, Xunli |
author_sort | Hassan, Sammer-ul |
collection | PubMed |
description | Miniaturized quantitative assays offer multiplexing capability in a microfluidic device for high-throughput applications such as antimicrobial resistance (AMR) studies. The detection of these multiple microchannels in a single microfluidic device becomes crucial for point-of-care (POC) testing and clinical diagnostics. This paper showcases an optical flow cell for detection of parallel microchannels in a microfluidic chip. The flow cell operates by measuring the light intensity from the microchannels based on Beer-Lambert law in a linearly moving chip. While this platform could be tailored for a wide variety of applications, here we show the design, fabrication and working principle of the device. β-lactamase, an indicator of bacterial resistance to β-lactam antibiotics, especially in milk, is shown as an example. The flow cell has a small footprint and uses low-powered, low-cost components, which makes it ideally suited for use in portable devices that require multiple sample detection in a single chip. |
format | Online Article Text |
id | pubmed-7230666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72306662020-05-22 Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels Hassan, Sammer-ul Zhang, Xunli Micromachines (Basel) Article Miniaturized quantitative assays offer multiplexing capability in a microfluidic device for high-throughput applications such as antimicrobial resistance (AMR) studies. The detection of these multiple microchannels in a single microfluidic device becomes crucial for point-of-care (POC) testing and clinical diagnostics. This paper showcases an optical flow cell for detection of parallel microchannels in a microfluidic chip. The flow cell operates by measuring the light intensity from the microchannels based on Beer-Lambert law in a linearly moving chip. While this platform could be tailored for a wide variety of applications, here we show the design, fabrication and working principle of the device. β-lactamase, an indicator of bacterial resistance to β-lactam antibiotics, especially in milk, is shown as an example. The flow cell has a small footprint and uses low-powered, low-cost components, which makes it ideally suited for use in portable devices that require multiple sample detection in a single chip. MDPI 2020-04-05 /pmc/articles/PMC7230666/ /pubmed/32260509 http://dx.doi.org/10.3390/mi11040385 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hassan, Sammer-ul Zhang, Xunli Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels |
title | Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels |
title_full | Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels |
title_fullStr | Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels |
title_full_unstemmed | Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels |
title_short | Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels |
title_sort | design and fabrication of optical flow cell for multiplex detection of β-lactamase in microchannels |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230666/ https://www.ncbi.nlm.nih.gov/pubmed/32260509 http://dx.doi.org/10.3390/mi11040385 |
work_keys_str_mv | AT hassansammerul designandfabricationofopticalflowcellformultiplexdetectionofblactamaseinmicrochannels AT zhangxunli designandfabricationofopticalflowcellformultiplexdetectionofblactamaseinmicrochannels |