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Expansion of Rare and Harmful Lineages is Associated with Established Rheumatoid Arthritis

Objectives: To characterize the gut microbiota profile in rheumatoid arthritis (RA) patients and investigate its association with certain characteristics of RA. Patients and methods: A nested case–control cohort of 40 patients with RA and 40 sex-age matched controls was studied. Subjects with diabet...

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Autores principales: Mena-Vázquez, Natalia, Ruiz-Limón, Patricia, Moreno-Indias, Isabel, Manrique-Arija, Sara, Tinahones, Francisco J., Fernández-Nebro, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230781/
https://www.ncbi.nlm.nih.gov/pubmed/32272752
http://dx.doi.org/10.3390/jcm9041044
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author Mena-Vázquez, Natalia
Ruiz-Limón, Patricia
Moreno-Indias, Isabel
Manrique-Arija, Sara
Tinahones, Francisco J.
Fernández-Nebro, Antonio
author_facet Mena-Vázquez, Natalia
Ruiz-Limón, Patricia
Moreno-Indias, Isabel
Manrique-Arija, Sara
Tinahones, Francisco J.
Fernández-Nebro, Antonio
author_sort Mena-Vázquez, Natalia
collection PubMed
description Objectives: To characterize the gut microbiota profile in rheumatoid arthritis (RA) patients and investigate its association with certain characteristics of RA. Patients and methods: A nested case–control cohort of 40 patients with RA and 40 sex-age matched controls was studied. Subjects with diabetes, with any other inflammatory disease, practicing extreme diets, taking antibiotics, probiotics or under any new treatment for at least three months prior to sampling were excluded. The microbiota composition was determined by 16S rRNA pyrosequencing and bioinformatics analysis by Quantitative Insights Into Microbial Ecology (QIIME). Other variables included clinical-laboratory variables and average Disease Activity Score 28 points during the follow-up period. Multiple linear regression models were constructed to investigate the possible risk factors for the microbiota. Results: β-diversity data showed that patients tend to differ from healthy subjects according to their microbiota (p = 0.07). The analysis showed an increase in Collinsella aerofaciens, Sedimentibacter and Enterococcus genera in patients compared to controls, as well as a decrease in Dorea formicigenerans. Likewise, an increase in the activity of arginine deiminase was observed, which was found in approximately 90% of the RA genes of the genus Collinsela. The sequence number of Collinsella aerofaciens was independently associated with age (B (95%CI), −0.347 (−21.6, −2.1)), high ACPA (0.323 (27.4–390.0)) and smoking (0.300 (8.8–256.4)) in RA patients. In addition, we observed decreases in Sarcina, 02d06 and Porphyromonas bacterial lineages. Conclusion: Patients with RA present dysbiosis, resulting from an abundance of certain bacterial lineages and a decrease in others. These alterations could influence the maintenance of autoimmunity to this disease.
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spelling pubmed-72307812020-05-22 Expansion of Rare and Harmful Lineages is Associated with Established Rheumatoid Arthritis Mena-Vázquez, Natalia Ruiz-Limón, Patricia Moreno-Indias, Isabel Manrique-Arija, Sara Tinahones, Francisco J. Fernández-Nebro, Antonio J Clin Med Article Objectives: To characterize the gut microbiota profile in rheumatoid arthritis (RA) patients and investigate its association with certain characteristics of RA. Patients and methods: A nested case–control cohort of 40 patients with RA and 40 sex-age matched controls was studied. Subjects with diabetes, with any other inflammatory disease, practicing extreme diets, taking antibiotics, probiotics or under any new treatment for at least three months prior to sampling were excluded. The microbiota composition was determined by 16S rRNA pyrosequencing and bioinformatics analysis by Quantitative Insights Into Microbial Ecology (QIIME). Other variables included clinical-laboratory variables and average Disease Activity Score 28 points during the follow-up period. Multiple linear regression models were constructed to investigate the possible risk factors for the microbiota. Results: β-diversity data showed that patients tend to differ from healthy subjects according to their microbiota (p = 0.07). The analysis showed an increase in Collinsella aerofaciens, Sedimentibacter and Enterococcus genera in patients compared to controls, as well as a decrease in Dorea formicigenerans. Likewise, an increase in the activity of arginine deiminase was observed, which was found in approximately 90% of the RA genes of the genus Collinsela. The sequence number of Collinsella aerofaciens was independently associated with age (B (95%CI), −0.347 (−21.6, −2.1)), high ACPA (0.323 (27.4–390.0)) and smoking (0.300 (8.8–256.4)) in RA patients. In addition, we observed decreases in Sarcina, 02d06 and Porphyromonas bacterial lineages. Conclusion: Patients with RA present dysbiosis, resulting from an abundance of certain bacterial lineages and a decrease in others. These alterations could influence the maintenance of autoimmunity to this disease. MDPI 2020-04-07 /pmc/articles/PMC7230781/ /pubmed/32272752 http://dx.doi.org/10.3390/jcm9041044 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mena-Vázquez, Natalia
Ruiz-Limón, Patricia
Moreno-Indias, Isabel
Manrique-Arija, Sara
Tinahones, Francisco J.
Fernández-Nebro, Antonio
Expansion of Rare and Harmful Lineages is Associated with Established Rheumatoid Arthritis
title Expansion of Rare and Harmful Lineages is Associated with Established Rheumatoid Arthritis
title_full Expansion of Rare and Harmful Lineages is Associated with Established Rheumatoid Arthritis
title_fullStr Expansion of Rare and Harmful Lineages is Associated with Established Rheumatoid Arthritis
title_full_unstemmed Expansion of Rare and Harmful Lineages is Associated with Established Rheumatoid Arthritis
title_short Expansion of Rare and Harmful Lineages is Associated with Established Rheumatoid Arthritis
title_sort expansion of rare and harmful lineages is associated with established rheumatoid arthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230781/
https://www.ncbi.nlm.nih.gov/pubmed/32272752
http://dx.doi.org/10.3390/jcm9041044
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