Cargando…
Indole-3-Carbinol Inhibits Citrobacter rodentium Infection through Multiple Pathways Including Reduction of Bacterial Adhesion and Enhancement of Cytotoxic T Cell Activity
Intestinal inflammation is associated with an increased risk of developing colorectal cancer and may result from dysregulated responses to commensal bacteria or exposure to bacterial pathogens. Dietary modulation of intestinal inflammation may protect against development of colon cancer. However, th...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230886/ https://www.ncbi.nlm.nih.gov/pubmed/32230738 http://dx.doi.org/10.3390/nu12040917 |
_version_ | 1783535061392424960 |
---|---|
author | Wu, Yanbei He, Qiang Yu, Liangli Pham, Quynhchi Cheung, Lumei Kim, Young S. Wang, Thomas T. Y. Smith, Allen D. |
author_facet | Wu, Yanbei He, Qiang Yu, Liangli Pham, Quynhchi Cheung, Lumei Kim, Young S. Wang, Thomas T. Y. Smith, Allen D. |
author_sort | Wu, Yanbei |
collection | PubMed |
description | Intestinal inflammation is associated with an increased risk of developing colorectal cancer and may result from dysregulated responses to commensal bacteria or exposure to bacterial pathogens. Dietary modulation of intestinal inflammation may protect against development of colon cancer. However, the precise diet-derived components and underlying mechanisms remain elusive. Citrobacter rodentium (Cr) induces acute intestinal inflammation and has been used to study the role of inflammation in the susceptibility to colon cancer. Here we examine the effects of indole-3-carbinol (I3C), a dietary compound with anticarcinogenic properties, on intestinal immune and inflammatory responses to Cr infection and adhesion to colonic cells in vitro. C57BL/6J mice were fed a diet with/without 1 μmol/g I3C and infected with Cr. Compared to infected mice fed with a control diet, consumption of a 1 μmol I3C/g diet significantly reduced fecal excretion of Cr, Cr colonization of the colon, and reduced colon crypt hyperplasia. Furthermore, expression of Cr-induced inflammatory markers such as IL-17A, IL-6, and IL1β were attenuated in infected mice fed with the I3C diet, compared to mice fed a control diet. The expression of cytotoxic T cell markers CD8 and FasL mRNA were increased in I3C-fed infected mice. In-vitro, I3C inhibited Cr growth and adhesion to Caco-2 cells. I3C alleviates Cr-induced murine colitis through multiple mechanisms including inhibition of Cr growth and adhesion to colonic cells in vitro and enhancement of cytotoxic T cell activity. |
format | Online Article Text |
id | pubmed-7230886 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72308862020-05-22 Indole-3-Carbinol Inhibits Citrobacter rodentium Infection through Multiple Pathways Including Reduction of Bacterial Adhesion and Enhancement of Cytotoxic T Cell Activity Wu, Yanbei He, Qiang Yu, Liangli Pham, Quynhchi Cheung, Lumei Kim, Young S. Wang, Thomas T. Y. Smith, Allen D. Nutrients Article Intestinal inflammation is associated with an increased risk of developing colorectal cancer and may result from dysregulated responses to commensal bacteria or exposure to bacterial pathogens. Dietary modulation of intestinal inflammation may protect against development of colon cancer. However, the precise diet-derived components and underlying mechanisms remain elusive. Citrobacter rodentium (Cr) induces acute intestinal inflammation and has been used to study the role of inflammation in the susceptibility to colon cancer. Here we examine the effects of indole-3-carbinol (I3C), a dietary compound with anticarcinogenic properties, on intestinal immune and inflammatory responses to Cr infection and adhesion to colonic cells in vitro. C57BL/6J mice were fed a diet with/without 1 μmol/g I3C and infected with Cr. Compared to infected mice fed with a control diet, consumption of a 1 μmol I3C/g diet significantly reduced fecal excretion of Cr, Cr colonization of the colon, and reduced colon crypt hyperplasia. Furthermore, expression of Cr-induced inflammatory markers such as IL-17A, IL-6, and IL1β were attenuated in infected mice fed with the I3C diet, compared to mice fed a control diet. The expression of cytotoxic T cell markers CD8 and FasL mRNA were increased in I3C-fed infected mice. In-vitro, I3C inhibited Cr growth and adhesion to Caco-2 cells. I3C alleviates Cr-induced murine colitis through multiple mechanisms including inhibition of Cr growth and adhesion to colonic cells in vitro and enhancement of cytotoxic T cell activity. MDPI 2020-03-27 /pmc/articles/PMC7230886/ /pubmed/32230738 http://dx.doi.org/10.3390/nu12040917 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wu, Yanbei He, Qiang Yu, Liangli Pham, Quynhchi Cheung, Lumei Kim, Young S. Wang, Thomas T. Y. Smith, Allen D. Indole-3-Carbinol Inhibits Citrobacter rodentium Infection through Multiple Pathways Including Reduction of Bacterial Adhesion and Enhancement of Cytotoxic T Cell Activity |
title | Indole-3-Carbinol Inhibits Citrobacter
rodentium Infection through Multiple Pathways Including Reduction of Bacterial Adhesion and Enhancement of Cytotoxic T Cell Activity |
title_full | Indole-3-Carbinol Inhibits Citrobacter
rodentium Infection through Multiple Pathways Including Reduction of Bacterial Adhesion and Enhancement of Cytotoxic T Cell Activity |
title_fullStr | Indole-3-Carbinol Inhibits Citrobacter
rodentium Infection through Multiple Pathways Including Reduction of Bacterial Adhesion and Enhancement of Cytotoxic T Cell Activity |
title_full_unstemmed | Indole-3-Carbinol Inhibits Citrobacter
rodentium Infection through Multiple Pathways Including Reduction of Bacterial Adhesion and Enhancement of Cytotoxic T Cell Activity |
title_short | Indole-3-Carbinol Inhibits Citrobacter
rodentium Infection through Multiple Pathways Including Reduction of Bacterial Adhesion and Enhancement of Cytotoxic T Cell Activity |
title_sort | indole-3-carbinol inhibits citrobacter
rodentium infection through multiple pathways including reduction of bacterial adhesion and enhancement of cytotoxic t cell activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230886/ https://www.ncbi.nlm.nih.gov/pubmed/32230738 http://dx.doi.org/10.3390/nu12040917 |
work_keys_str_mv | AT wuyanbei indole3carbinolinhibitscitrobacterrodentiuminfectionthroughmultiplepathwaysincludingreductionofbacterialadhesionandenhancementofcytotoxictcellactivity AT heqiang indole3carbinolinhibitscitrobacterrodentiuminfectionthroughmultiplepathwaysincludingreductionofbacterialadhesionandenhancementofcytotoxictcellactivity AT yuliangli indole3carbinolinhibitscitrobacterrodentiuminfectionthroughmultiplepathwaysincludingreductionofbacterialadhesionandenhancementofcytotoxictcellactivity AT phamquynhchi indole3carbinolinhibitscitrobacterrodentiuminfectionthroughmultiplepathwaysincludingreductionofbacterialadhesionandenhancementofcytotoxictcellactivity AT cheunglumei indole3carbinolinhibitscitrobacterrodentiuminfectionthroughmultiplepathwaysincludingreductionofbacterialadhesionandenhancementofcytotoxictcellactivity AT kimyoungs indole3carbinolinhibitscitrobacterrodentiuminfectionthroughmultiplepathwaysincludingreductionofbacterialadhesionandenhancementofcytotoxictcellactivity AT wangthomasty indole3carbinolinhibitscitrobacterrodentiuminfectionthroughmultiplepathwaysincludingreductionofbacterialadhesionandenhancementofcytotoxictcellactivity AT smithallend indole3carbinolinhibitscitrobacterrodentiuminfectionthroughmultiplepathwaysincludingreductionofbacterialadhesionandenhancementofcytotoxictcellactivity |