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The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study

Recent evidence shows that rooibos compounds, aspalathin and phenylpyruvic acid-2-O-β-d-glucoside (PPAG), can independently protect cardiomyocytes from hyperglycemia-related reactive oxygen species (ROS). While aspalathin shows more potency by enhancing intracellular antioxidant defenses, PPAG acts...

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Autores principales: Dludla, Phiwayinkosi V., Muller, Christo J. F., Louw, Johan, Mazibuko-Mbeje, Sithandiwe E., Tiano, Luca, Silvestri, Sonia, Orlando, Patrick, Marcheggiani, Fabio, Cirilli, Ilenia, Chellan, Nireshni, Ghoor, Samira, Nkambule, Bongani B., Essop, M. Faadiel, Huisamen, Barbara, Johnson, Rabia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231041/
https://www.ncbi.nlm.nih.gov/pubmed/32325968
http://dx.doi.org/10.3390/nu12041151
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author Dludla, Phiwayinkosi V.
Muller, Christo J. F.
Louw, Johan
Mazibuko-Mbeje, Sithandiwe E.
Tiano, Luca
Silvestri, Sonia
Orlando, Patrick
Marcheggiani, Fabio
Cirilli, Ilenia
Chellan, Nireshni
Ghoor, Samira
Nkambule, Bongani B.
Essop, M. Faadiel
Huisamen, Barbara
Johnson, Rabia
author_facet Dludla, Phiwayinkosi V.
Muller, Christo J. F.
Louw, Johan
Mazibuko-Mbeje, Sithandiwe E.
Tiano, Luca
Silvestri, Sonia
Orlando, Patrick
Marcheggiani, Fabio
Cirilli, Ilenia
Chellan, Nireshni
Ghoor, Samira
Nkambule, Bongani B.
Essop, M. Faadiel
Huisamen, Barbara
Johnson, Rabia
author_sort Dludla, Phiwayinkosi V.
collection PubMed
description Recent evidence shows that rooibos compounds, aspalathin and phenylpyruvic acid-2-O-β-d-glucoside (PPAG), can independently protect cardiomyocytes from hyperglycemia-related reactive oxygen species (ROS). While aspalathin shows more potency by enhancing intracellular antioxidant defenses, PPAG acts more as an anti-apoptotic agent. Thus, to further understand the protective capabilities of these compounds against hyperglycemia-induced cardiac damage, their combinatory effect was investigated and compared to metformin. An in vitro model of H9c2 cardiomyocytes exposed to chronic glucose concentrations was employed to study the impact of such compounds on hyperglycemia-induced damage. Here, high glucose exposure impaired myocardial substrate utilization by abnormally enhancing free fatty acid oxidation while concomitantly suppressing glucose oxidation. This was paralleled by altered expression of genes involved in energy metabolism including acetyl-CoA carboxylase (ACC), 5′ AMP-activated protein kinase (AMPK), and peroxisome proliferator-activated receptor-alpha (PPARα). The combination treatment improved myocardial substrate metabolism, maintained mitochondrial membrane potential, and attenuated various markers for oxidative stress including nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and glutathione content. It also showed a much-improved effect by ameliorating DNA damage when compared to metformin. The current study demonstrates that rooibos compounds offer unique cardioprotective properties against hyperglycemia-induced and potentially against diabetes-induced cardiac damage. These data also support further exploration of rooibos compounds to better assess the cardioprotective effects of different bioactive compound combinations.
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spelling pubmed-72310412020-05-22 The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study Dludla, Phiwayinkosi V. Muller, Christo J. F. Louw, Johan Mazibuko-Mbeje, Sithandiwe E. Tiano, Luca Silvestri, Sonia Orlando, Patrick Marcheggiani, Fabio Cirilli, Ilenia Chellan, Nireshni Ghoor, Samira Nkambule, Bongani B. Essop, M. Faadiel Huisamen, Barbara Johnson, Rabia Nutrients Article Recent evidence shows that rooibos compounds, aspalathin and phenylpyruvic acid-2-O-β-d-glucoside (PPAG), can independently protect cardiomyocytes from hyperglycemia-related reactive oxygen species (ROS). While aspalathin shows more potency by enhancing intracellular antioxidant defenses, PPAG acts more as an anti-apoptotic agent. Thus, to further understand the protective capabilities of these compounds against hyperglycemia-induced cardiac damage, their combinatory effect was investigated and compared to metformin. An in vitro model of H9c2 cardiomyocytes exposed to chronic glucose concentrations was employed to study the impact of such compounds on hyperglycemia-induced damage. Here, high glucose exposure impaired myocardial substrate utilization by abnormally enhancing free fatty acid oxidation while concomitantly suppressing glucose oxidation. This was paralleled by altered expression of genes involved in energy metabolism including acetyl-CoA carboxylase (ACC), 5′ AMP-activated protein kinase (AMPK), and peroxisome proliferator-activated receptor-alpha (PPARα). The combination treatment improved myocardial substrate metabolism, maintained mitochondrial membrane potential, and attenuated various markers for oxidative stress including nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and glutathione content. It also showed a much-improved effect by ameliorating DNA damage when compared to metformin. The current study demonstrates that rooibos compounds offer unique cardioprotective properties against hyperglycemia-induced and potentially against diabetes-induced cardiac damage. These data also support further exploration of rooibos compounds to better assess the cardioprotective effects of different bioactive compound combinations. MDPI 2020-04-20 /pmc/articles/PMC7231041/ /pubmed/32325968 http://dx.doi.org/10.3390/nu12041151 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dludla, Phiwayinkosi V.
Muller, Christo J. F.
Louw, Johan
Mazibuko-Mbeje, Sithandiwe E.
Tiano, Luca
Silvestri, Sonia
Orlando, Patrick
Marcheggiani, Fabio
Cirilli, Ilenia
Chellan, Nireshni
Ghoor, Samira
Nkambule, Bongani B.
Essop, M. Faadiel
Huisamen, Barbara
Johnson, Rabia
The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study
title The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study
title_full The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study
title_fullStr The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study
title_full_unstemmed The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study
title_short The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study
title_sort combination effect of aspalathin and phenylpyruvic acid-2-o-β-d-glucoside from rooibos against hyperglycemia-induced cardiac damage: an in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231041/
https://www.ncbi.nlm.nih.gov/pubmed/32325968
http://dx.doi.org/10.3390/nu12041151
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