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The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study
Recent evidence shows that rooibos compounds, aspalathin and phenylpyruvic acid-2-O-β-d-glucoside (PPAG), can independently protect cardiomyocytes from hyperglycemia-related reactive oxygen species (ROS). While aspalathin shows more potency by enhancing intracellular antioxidant defenses, PPAG acts...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231041/ https://www.ncbi.nlm.nih.gov/pubmed/32325968 http://dx.doi.org/10.3390/nu12041151 |
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author | Dludla, Phiwayinkosi V. Muller, Christo J. F. Louw, Johan Mazibuko-Mbeje, Sithandiwe E. Tiano, Luca Silvestri, Sonia Orlando, Patrick Marcheggiani, Fabio Cirilli, Ilenia Chellan, Nireshni Ghoor, Samira Nkambule, Bongani B. Essop, M. Faadiel Huisamen, Barbara Johnson, Rabia |
author_facet | Dludla, Phiwayinkosi V. Muller, Christo J. F. Louw, Johan Mazibuko-Mbeje, Sithandiwe E. Tiano, Luca Silvestri, Sonia Orlando, Patrick Marcheggiani, Fabio Cirilli, Ilenia Chellan, Nireshni Ghoor, Samira Nkambule, Bongani B. Essop, M. Faadiel Huisamen, Barbara Johnson, Rabia |
author_sort | Dludla, Phiwayinkosi V. |
collection | PubMed |
description | Recent evidence shows that rooibos compounds, aspalathin and phenylpyruvic acid-2-O-β-d-glucoside (PPAG), can independently protect cardiomyocytes from hyperglycemia-related reactive oxygen species (ROS). While aspalathin shows more potency by enhancing intracellular antioxidant defenses, PPAG acts more as an anti-apoptotic agent. Thus, to further understand the protective capabilities of these compounds against hyperglycemia-induced cardiac damage, their combinatory effect was investigated and compared to metformin. An in vitro model of H9c2 cardiomyocytes exposed to chronic glucose concentrations was employed to study the impact of such compounds on hyperglycemia-induced damage. Here, high glucose exposure impaired myocardial substrate utilization by abnormally enhancing free fatty acid oxidation while concomitantly suppressing glucose oxidation. This was paralleled by altered expression of genes involved in energy metabolism including acetyl-CoA carboxylase (ACC), 5′ AMP-activated protein kinase (AMPK), and peroxisome proliferator-activated receptor-alpha (PPARα). The combination treatment improved myocardial substrate metabolism, maintained mitochondrial membrane potential, and attenuated various markers for oxidative stress including nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and glutathione content. It also showed a much-improved effect by ameliorating DNA damage when compared to metformin. The current study demonstrates that rooibos compounds offer unique cardioprotective properties against hyperglycemia-induced and potentially against diabetes-induced cardiac damage. These data also support further exploration of rooibos compounds to better assess the cardioprotective effects of different bioactive compound combinations. |
format | Online Article Text |
id | pubmed-7231041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72310412020-05-22 The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study Dludla, Phiwayinkosi V. Muller, Christo J. F. Louw, Johan Mazibuko-Mbeje, Sithandiwe E. Tiano, Luca Silvestri, Sonia Orlando, Patrick Marcheggiani, Fabio Cirilli, Ilenia Chellan, Nireshni Ghoor, Samira Nkambule, Bongani B. Essop, M. Faadiel Huisamen, Barbara Johnson, Rabia Nutrients Article Recent evidence shows that rooibos compounds, aspalathin and phenylpyruvic acid-2-O-β-d-glucoside (PPAG), can independently protect cardiomyocytes from hyperglycemia-related reactive oxygen species (ROS). While aspalathin shows more potency by enhancing intracellular antioxidant defenses, PPAG acts more as an anti-apoptotic agent. Thus, to further understand the protective capabilities of these compounds against hyperglycemia-induced cardiac damage, their combinatory effect was investigated and compared to metformin. An in vitro model of H9c2 cardiomyocytes exposed to chronic glucose concentrations was employed to study the impact of such compounds on hyperglycemia-induced damage. Here, high glucose exposure impaired myocardial substrate utilization by abnormally enhancing free fatty acid oxidation while concomitantly suppressing glucose oxidation. This was paralleled by altered expression of genes involved in energy metabolism including acetyl-CoA carboxylase (ACC), 5′ AMP-activated protein kinase (AMPK), and peroxisome proliferator-activated receptor-alpha (PPARα). The combination treatment improved myocardial substrate metabolism, maintained mitochondrial membrane potential, and attenuated various markers for oxidative stress including nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and glutathione content. It also showed a much-improved effect by ameliorating DNA damage when compared to metformin. The current study demonstrates that rooibos compounds offer unique cardioprotective properties against hyperglycemia-induced and potentially against diabetes-induced cardiac damage. These data also support further exploration of rooibos compounds to better assess the cardioprotective effects of different bioactive compound combinations. MDPI 2020-04-20 /pmc/articles/PMC7231041/ /pubmed/32325968 http://dx.doi.org/10.3390/nu12041151 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dludla, Phiwayinkosi V. Muller, Christo J. F. Louw, Johan Mazibuko-Mbeje, Sithandiwe E. Tiano, Luca Silvestri, Sonia Orlando, Patrick Marcheggiani, Fabio Cirilli, Ilenia Chellan, Nireshni Ghoor, Samira Nkambule, Bongani B. Essop, M. Faadiel Huisamen, Barbara Johnson, Rabia The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study |
title | The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study |
title_full | The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study |
title_fullStr | The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study |
title_full_unstemmed | The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study |
title_short | The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study |
title_sort | combination effect of aspalathin and phenylpyruvic acid-2-o-β-d-glucoside from rooibos against hyperglycemia-induced cardiac damage: an in vitro study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231041/ https://www.ncbi.nlm.nih.gov/pubmed/32325968 http://dx.doi.org/10.3390/nu12041151 |
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