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Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence

Background: Recent studies have supported the beneficial effects of a short abstinence period on sperm parameters. The aim of this study was to assess sperm motility, morphology and DNA fragmentation before and after swim-up of a second ejaculate obtained after a short abstinence period in normozoos...

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Autores principales: Manna, Claudio, Barbagallo, Federica, Manzo, Raffaella, Rahman, Ashraf, Francomano, Davide, Calogero, Aldo E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231087/
https://www.ncbi.nlm.nih.gov/pubmed/32260592
http://dx.doi.org/10.3390/jcm9041029
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author Manna, Claudio
Barbagallo, Federica
Manzo, Raffaella
Rahman, Ashraf
Francomano, Davide
Calogero, Aldo E.
author_facet Manna, Claudio
Barbagallo, Federica
Manzo, Raffaella
Rahman, Ashraf
Francomano, Davide
Calogero, Aldo E.
author_sort Manna, Claudio
collection PubMed
description Background: Recent studies have supported the beneficial effects of a short abstinence period on sperm parameters. The aim of this study was to assess sperm motility, morphology and DNA fragmentation before and after swim-up of a second ejaculate obtained after a short abstinence period in normozoospermic men and oligo-astheno-teratozoospermic (OAT) patients. Material and methods: Semen analyses and swim-up preparations of two consecutive semen samples (collected within 1 h) were carried out in 30 normozoospermic and 35 OAT patients enrolled in an assisted reproductive technique (ART) program. Results: Compared to the first ejaculate, the second sample showed a higher percentage of spermatozoa with normal form (p < 0.01) and lower percentage of spermatozoa with DNA fragmentation (p < 0.01) in normozoospermic men, whereas a higher percentage of spermatozoa with progressive motility (p < 0.001) and normal morphology (p < 0.0001) was found in OAT patients. Swim-up separation showed a lower DNA fragmentation rate (p < 0.05) in the second ejaculate in normozoospermic men, whereas the second ejaculate of OAT patents showed an increase in normally-shaped spermatozoa (p < 0.01) and lower percentage of spermatozoa with fragmented DNA (p < 0.001) compared to the first one. Conclusions: Swim-up separation of a second ejaculate collected within 1 h might be suggested for ART procedures, especially in OAT patients.
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spelling pubmed-72310872020-05-22 Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence Manna, Claudio Barbagallo, Federica Manzo, Raffaella Rahman, Ashraf Francomano, Davide Calogero, Aldo E. J Clin Med Article Background: Recent studies have supported the beneficial effects of a short abstinence period on sperm parameters. The aim of this study was to assess sperm motility, morphology and DNA fragmentation before and after swim-up of a second ejaculate obtained after a short abstinence period in normozoospermic men and oligo-astheno-teratozoospermic (OAT) patients. Material and methods: Semen analyses and swim-up preparations of two consecutive semen samples (collected within 1 h) were carried out in 30 normozoospermic and 35 OAT patients enrolled in an assisted reproductive technique (ART) program. Results: Compared to the first ejaculate, the second sample showed a higher percentage of spermatozoa with normal form (p < 0.01) and lower percentage of spermatozoa with DNA fragmentation (p < 0.01) in normozoospermic men, whereas a higher percentage of spermatozoa with progressive motility (p < 0.001) and normal morphology (p < 0.0001) was found in OAT patients. Swim-up separation showed a lower DNA fragmentation rate (p < 0.05) in the second ejaculate in normozoospermic men, whereas the second ejaculate of OAT patents showed an increase in normally-shaped spermatozoa (p < 0.01) and lower percentage of spermatozoa with fragmented DNA (p < 0.001) compared to the first one. Conclusions: Swim-up separation of a second ejaculate collected within 1 h might be suggested for ART procedures, especially in OAT patients. MDPI 2020-04-05 /pmc/articles/PMC7231087/ /pubmed/32260592 http://dx.doi.org/10.3390/jcm9041029 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Manna, Claudio
Barbagallo, Federica
Manzo, Raffaella
Rahman, Ashraf
Francomano, Davide
Calogero, Aldo E.
Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence
title Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence
title_full Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence
title_fullStr Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence
title_full_unstemmed Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence
title_short Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence
title_sort sperm parameters before and after swim-up of a second ejaculate after a short period of abstinence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231087/
https://www.ncbi.nlm.nih.gov/pubmed/32260592
http://dx.doi.org/10.3390/jcm9041029
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