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The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli
A direct link between DNA replication regulation and central carbon metabolism (CCM) has been previously demonstrated in Bacillus subtilis and Escherichia coli, as effects of certain mutations in genes coding for replication proteins could be specifically suppressed by particular mutations in genes...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231150/ https://www.ncbi.nlm.nih.gov/pubmed/32325866 http://dx.doi.org/10.3390/genes11040447 |
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author | Krause, Klaudyna Maciąg-Dorszyńska, Monika Wosinski, Anna Gaffke, Lidia Morcinek-Orłowska, Joanna Rintz, Estera Bielańska, Patrycja Szalewska-Pałasz, Agnieszka Muskhelishvili, Georgi Węgrzyn, Grzegorz |
author_facet | Krause, Klaudyna Maciąg-Dorszyńska, Monika Wosinski, Anna Gaffke, Lidia Morcinek-Orłowska, Joanna Rintz, Estera Bielańska, Patrycja Szalewska-Pałasz, Agnieszka Muskhelishvili, Georgi Węgrzyn, Grzegorz |
author_sort | Krause, Klaudyna |
collection | PubMed |
description | A direct link between DNA replication regulation and central carbon metabolism (CCM) has been previously demonstrated in Bacillus subtilis and Escherichia coli, as effects of certain mutations in genes coding for replication proteins could be specifically suppressed by particular mutations in genes encoding CCM enzymes. However, specific molecular mechanism(s) of this link remained unknown. In this report, we demonstrate that various CCM metabolites can suppress the effects of mutations in different replication genes of E. coli on bacterial growth, cell morphology, and nucleoid localization. This provides evidence that the CCM-replication link is mediated by metabolites rather than direct protein-protein interactions. On the other hand, action of metabolites on DNA replication appears indirect rather than based on direct influence on the replication machinery, as rate of DNA synthesis could not be corrected by metabolites in short-term experiments. This corroborates the recent discovery that in B. subtilis, there are multiple links connecting CCM to DNA replication initiation and elongation. Therefore, one may suggest that although different in detail, the molecular mechanisms of CCM-dependent regulation of DNA replication are similar in E. coli and B. subtilis, making this regulation an important and common constituent of the control of cell physiology in bacteria. |
format | Online Article Text |
id | pubmed-7231150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72311502020-05-22 The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli Krause, Klaudyna Maciąg-Dorszyńska, Monika Wosinski, Anna Gaffke, Lidia Morcinek-Orłowska, Joanna Rintz, Estera Bielańska, Patrycja Szalewska-Pałasz, Agnieszka Muskhelishvili, Georgi Węgrzyn, Grzegorz Genes (Basel) Article A direct link between DNA replication regulation and central carbon metabolism (CCM) has been previously demonstrated in Bacillus subtilis and Escherichia coli, as effects of certain mutations in genes coding for replication proteins could be specifically suppressed by particular mutations in genes encoding CCM enzymes. However, specific molecular mechanism(s) of this link remained unknown. In this report, we demonstrate that various CCM metabolites can suppress the effects of mutations in different replication genes of E. coli on bacterial growth, cell morphology, and nucleoid localization. This provides evidence that the CCM-replication link is mediated by metabolites rather than direct protein-protein interactions. On the other hand, action of metabolites on DNA replication appears indirect rather than based on direct influence on the replication machinery, as rate of DNA synthesis could not be corrected by metabolites in short-term experiments. This corroborates the recent discovery that in B. subtilis, there are multiple links connecting CCM to DNA replication initiation and elongation. Therefore, one may suggest that although different in detail, the molecular mechanisms of CCM-dependent regulation of DNA replication are similar in E. coli and B. subtilis, making this regulation an important and common constituent of the control of cell physiology in bacteria. MDPI 2020-04-19 /pmc/articles/PMC7231150/ /pubmed/32325866 http://dx.doi.org/10.3390/genes11040447 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Krause, Klaudyna Maciąg-Dorszyńska, Monika Wosinski, Anna Gaffke, Lidia Morcinek-Orłowska, Joanna Rintz, Estera Bielańska, Patrycja Szalewska-Pałasz, Agnieszka Muskhelishvili, Georgi Węgrzyn, Grzegorz The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli |
title | The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli |
title_full | The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli |
title_fullStr | The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli |
title_full_unstemmed | The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli |
title_short | The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli |
title_sort | role of metabolites in the link between dna replication and central carbon metabolism in escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231150/ https://www.ncbi.nlm.nih.gov/pubmed/32325866 http://dx.doi.org/10.3390/genes11040447 |
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