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The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli

A direct link between DNA replication regulation and central carbon metabolism (CCM) has been previously demonstrated in Bacillus subtilis and Escherichia coli, as effects of certain mutations in genes coding for replication proteins could be specifically suppressed by particular mutations in genes...

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Autores principales: Krause, Klaudyna, Maciąg-Dorszyńska, Monika, Wosinski, Anna, Gaffke, Lidia, Morcinek-Orłowska, Joanna, Rintz, Estera, Bielańska, Patrycja, Szalewska-Pałasz, Agnieszka, Muskhelishvili, Georgi, Węgrzyn, Grzegorz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231150/
https://www.ncbi.nlm.nih.gov/pubmed/32325866
http://dx.doi.org/10.3390/genes11040447
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author Krause, Klaudyna
Maciąg-Dorszyńska, Monika
Wosinski, Anna
Gaffke, Lidia
Morcinek-Orłowska, Joanna
Rintz, Estera
Bielańska, Patrycja
Szalewska-Pałasz, Agnieszka
Muskhelishvili, Georgi
Węgrzyn, Grzegorz
author_facet Krause, Klaudyna
Maciąg-Dorszyńska, Monika
Wosinski, Anna
Gaffke, Lidia
Morcinek-Orłowska, Joanna
Rintz, Estera
Bielańska, Patrycja
Szalewska-Pałasz, Agnieszka
Muskhelishvili, Georgi
Węgrzyn, Grzegorz
author_sort Krause, Klaudyna
collection PubMed
description A direct link between DNA replication regulation and central carbon metabolism (CCM) has been previously demonstrated in Bacillus subtilis and Escherichia coli, as effects of certain mutations in genes coding for replication proteins could be specifically suppressed by particular mutations in genes encoding CCM enzymes. However, specific molecular mechanism(s) of this link remained unknown. In this report, we demonstrate that various CCM metabolites can suppress the effects of mutations in different replication genes of E. coli on bacterial growth, cell morphology, and nucleoid localization. This provides evidence that the CCM-replication link is mediated by metabolites rather than direct protein-protein interactions. On the other hand, action of metabolites on DNA replication appears indirect rather than based on direct influence on the replication machinery, as rate of DNA synthesis could not be corrected by metabolites in short-term experiments. This corroborates the recent discovery that in B. subtilis, there are multiple links connecting CCM to DNA replication initiation and elongation. Therefore, one may suggest that although different in detail, the molecular mechanisms of CCM-dependent regulation of DNA replication are similar in E. coli and B. subtilis, making this regulation an important and common constituent of the control of cell physiology in bacteria.
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spelling pubmed-72311502020-05-22 The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli Krause, Klaudyna Maciąg-Dorszyńska, Monika Wosinski, Anna Gaffke, Lidia Morcinek-Orłowska, Joanna Rintz, Estera Bielańska, Patrycja Szalewska-Pałasz, Agnieszka Muskhelishvili, Georgi Węgrzyn, Grzegorz Genes (Basel) Article A direct link between DNA replication regulation and central carbon metabolism (CCM) has been previously demonstrated in Bacillus subtilis and Escherichia coli, as effects of certain mutations in genes coding for replication proteins could be specifically suppressed by particular mutations in genes encoding CCM enzymes. However, specific molecular mechanism(s) of this link remained unknown. In this report, we demonstrate that various CCM metabolites can suppress the effects of mutations in different replication genes of E. coli on bacterial growth, cell morphology, and nucleoid localization. This provides evidence that the CCM-replication link is mediated by metabolites rather than direct protein-protein interactions. On the other hand, action of metabolites on DNA replication appears indirect rather than based on direct influence on the replication machinery, as rate of DNA synthesis could not be corrected by metabolites in short-term experiments. This corroborates the recent discovery that in B. subtilis, there are multiple links connecting CCM to DNA replication initiation and elongation. Therefore, one may suggest that although different in detail, the molecular mechanisms of CCM-dependent regulation of DNA replication are similar in E. coli and B. subtilis, making this regulation an important and common constituent of the control of cell physiology in bacteria. MDPI 2020-04-19 /pmc/articles/PMC7231150/ /pubmed/32325866 http://dx.doi.org/10.3390/genes11040447 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krause, Klaudyna
Maciąg-Dorszyńska, Monika
Wosinski, Anna
Gaffke, Lidia
Morcinek-Orłowska, Joanna
Rintz, Estera
Bielańska, Patrycja
Szalewska-Pałasz, Agnieszka
Muskhelishvili, Georgi
Węgrzyn, Grzegorz
The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli
title The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli
title_full The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli
title_fullStr The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli
title_full_unstemmed The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli
title_short The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli
title_sort role of metabolites in the link between dna replication and central carbon metabolism in escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231150/
https://www.ncbi.nlm.nih.gov/pubmed/32325866
http://dx.doi.org/10.3390/genes11040447
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